Protocol Handbook for Cancer Biology

1st Edition - February 12, 2021
Imprint: Academic Press
Editors: Gauri Misra, Jyotika Rajawat
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 0 0 0 6 - 5
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 8 9 8 1 7 - 1

Protocol Handbook for Cancer Biology brings together a comprehensive collection of the methods used for cancer assessment, diagnostics, and therapeutics. Various protocols… Read more

Purchase options

BLOOM INTO SPRING SAVINGS

Save up to 25% on books and eBooks!

Shop now

Institutional subscription on ScienceDirect

Request a sales quote

Protocol Handbook for Cancer Biology brings together a comprehensive collection of the methods used for cancer assessment, diagnostics, and therapeutics. Various protocols are discussed along with alternative strategies, including the advantages and limitations of techniques that have been used in labs globally. These protocols are presented by cancer biology experts based on their real-world experience. The protocols in this book will be a valuable resource for cancer researchers and graduate students, who can utilize the techniques described to conduct research more efficiently and successfully.

Cover image
Title page
Table of Contents
Copyright
Dedication
Contributors
Editor biographies
Foreword
Preface
Acknowledgments
Chapter 1. Overview of cancer assessment, diagnostics, and therapeutics
1.1. Introduction
1.2. Salient differences between normal and cancer cell
1.3. Hallmarks of cancer
1.4. Identification of cancer
1.5. Cancer therapeutics
Chapter 2. Cell viability and apoptotic assays to assess drug efficacy
2.1. Trypan blue dye exclusion assay
2.2. Lactate dehydrogenase assay
2.3. Terminal deoxynucleotide transferase-mediated biotin-dUTP nick end labeling assay
2.4. Annexin V staining
2.5. Comet assay (single-cell gel electrophoresis)
2.6. Summary
Chapter 3. Assays to assess the proliferative behavior of cancer cells
3.1. Introduction
3.2. Cell cycle analysis using flow cytometry
3.3. Antibody-based cell proliferation assays
3.4. Metabolism-based cell proliferation assay
3.5. Clonogenic assay
3.6. Summary
Chapter 4. Assays to evaluate tumor angiogenesis
4.1. Introduction
4.2. Endothelial cell proliferation assay
4.3. Cellular motility assays
4.4. Endothelial cell tube formation assay
4.5. Aortic ring sprouting assay
4.6. Chorioallantoic membrane assay
4.7. Subcutaneous matrigel plug assay
4.8. Summary
4.9. Notes
Chapter 5. Assays and protocols for the detection of cancer metastasis
5.1. Introduction
5.2. In vitro assays
5.3. In vivo assays
5.4. Summary
Chapter 6. Isolation and characterization of cancer stem cells
6.1. Introduction
6.2. Isolation and characterization of CSCs using surface markers
6.3. Isolation and characterization of CSCs using nonimmunogenic techniques
6.4. Characterization of CSCs using in vitro and in vivo assays
6.5. Summary
6.6. Appendix
Chapter 7. Histopathology-based cancer detection methods
7.1. Introduction
7.2. Hematoxylin and eosin staining
7.3. Immunohistochemistry
7.4. Fluorescent in situ hybridization
Chapter 8. Molecular imaging in tumor diagnosis and treatment
8.1. Introduction
8.2. Modalities available for molecular tumor imaging
8.3. Summary
Abbreviations
Chapter 9. High throughput cell-based screening methods for cancer drug discovery
9.1. Introduction
9.2. In cell western assay
9.3. 3D multicellular spheroid model
9.4. Microfluidic platform for drug screening
9.5. Summary
Chapter 10. Nanotechnology in cancer therapeutics: cerium oxide nanomaterials as cancer therapeutic agents
10.1. Definition
10.2. Rationale
10.3. Materials, equipment, and reagents
10.4. Protocols
10.5. Precursor techniques
10.6. Safety considerations and standards
10.7. Analysis and statistics
10.8. Pros and cons
10.9. Alternative methods/procedures
10.10. Troubleshooting and optimization
10.11. Summary
Index

Gauri Misra

Gauri Misra, PhD, has a research focus in the field of cancer biology and Malaria directed toward drug discovery, armored with an experience in Structural Biology, Biophysics and Bioinformatics. She has an outstanding academic background and experience of more than 10 years in the field of teaching and research, adorned by various prestigious awards and honors at different stages of her career with a strong desire of transferring her repertoire of knowledge to the upcoming generations. Her PhD thesis was awarded with Eli Lilly Asia Outstanding Thesis Award (first prize) in 2009. She has received “state of the art” International exposure while working as a postdoctoral researcher at CHUL Research Centre, Canada and visiting scientist at ISPC, Weizmann Institute of Science, Rehovot, Israel in 2014 along with few other countries. She serves as a reviewer for various renowned international journals and is often invited for speaking engagements about her research. To date, she has authored 17 research articles, 4 international books, and 6 book chapters. The ongoing research project in her laboratory focuses on evaluating the potential of new lead compounds against breast cancer.

Affiliations and expertise

Scientist Grade-II, National Institute of Biologicals, (Autonomous Institute under Ministry of Health and Family Welfare), Government of India, Noida, Uttar Pradesh, India

Jyotika Rajawat

Jyotika Rajawat, PhD, researches in the field of cancer and drug discovery. She has a rich experience of 8 years in cancer research and teaching. Her postdoctoral research at CHUQ Research Centre, Canada, followed by research projects at National Centre for Cell Science (Pune), CSIR- Central Drug Research Institute (Lucknow), India has established a novel role of PARP1 protein in cancer progression. She has written several research articles, three book chapters, and newspaper articles. She is a member of the Editorial Board, IJBST journal and is a reviewer for various international publishers. She has been the recipient of several awards including DBT Bio-CARe and DST Women Scientist Award (awarded by the Govt. of India). She is also passionate about conducting cancer awareness workshops and literacy camps for the underprivileged especially in the rural areas. Dr. Rajawat being an expert in cancer biology understands the needs of readers and writes with those needs in mind.

Affiliations and expertise

Research Scientist, DST WOS-A project, Lucknow, Uttar Pradesh, India

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health