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Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the mu… Read more
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Immediately download your ebook while waiting for your print delivery. No promo code needed.
Proteomic Profiling and Analytical Chemistry: The Crossroads, Second Edition helps scientists without a strong background in analytical chemistry to understand principles of the multistep proteomic experiment necessary for its successful completion. It also helps researchers who do have an analytical chemistry background to break into the proteomics field. Highlighting points of junction between proteomics and analytical chemistry, this resource links experimental design with analytical measurements, data analysis, and quality control. This targeted point of view will help both biologists and chemists to better understand all components of a complex proteomic study.
The book provides detailed coverage of experimental aspects such as sample preparation, protein extraction and precipitation, gel electrophoresis, microarrays, dynamics of fluorescent dyes, and more. The key feature of this book is a direct link between multistep proteomic strategy and quality control routinely applied in analytical chemistry. This second edition features a new chapter on SWATH-MS, substantial updates to all chapters, including proteomic database search and analytical quantification, expanded discussion of post-hoc statistical tests, and additional content on validation in proteomics.
1. Introduction
2. Biomolecules
3. General Strategies for Proteomic Sample Preparation
4. Protein Extraction and Precipitation
5. Online and Offline Sample Fractionation
6. Immunoaffinity Depletion of Highly Abundant Proteins for Proteomic Sample Preparation
7. Gel Electrophoresis
8. Quantitative Measurements in Proteomics: Mass Spectrometry
9. SWATH-MS: Data Acquisition and Analysis
10. Top-Down Proteomics
11. Proteomic Database Search and Analytical Quantification for Mass Spectrometry
12. Design and Statistical Analysis of Mass-Spectrometry-Based Quantitative Proteomics Data
13. Principles of Analytical Validation
14. Validation in Proteomics and Regulatory Affairs
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