Proteins and Nucleic Acids

1st Edition - January 1, 1973

Editor: Isidore Gersh

Hardback ISBN:

9 7 8 - 0 - 1 2 - 2 8 1 4 0 1 - 3

eBook ISBN:

9 7 8 - 0 - 3 2 3 - 1 5 1 5 0 - 4

Submicroscopic Cytochemistry, Volume I: Proteins and Nucleic Acids presents laboratory findings and theoretical aspects involved or derived from submicroscopic cytochemistry study… Read more

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Submicroscopic Cytochemistry, Volume I: Proteins and Nucleic Acids presents laboratory findings and theoretical aspects involved or derived from submicroscopic cytochemistry study of proteins and nucleic acids. It is a two-volume book, encompassing 14 chapters covering three major topics: the pattern of distribution of nucleic acids at the molecular level in various cells and states of activity and in relation to protein synthesis; the molecular and macromolecular organization of cellular membranes; and the origin and distribution of the major macromolecular aggregates of connective tissue. After a brief introduction to the aspects of submicroscopic cytochemistry, the book discusses morphology, with emphasis on the distribution of cell components and their organization in cells at the macromolecular level. It then presents developed methods to preserve and selectively identify proteins, nucleic acids, lipids, acid mucopolysaccharides, and molecular aggregates very nearly in the position they occupied in the living state. Chapters 8 to 12 describe DNA molecular patterns during cell differentiation and cell maturation, and the alteration of these patterns in certain mouse cells. A discussion on DNA molecular patterns during metaplasia of stratified squamous mouse epithelium and of spinal ganglion cells during regeneration is provided. The concluding chapters examine DNA organization during mitotic cycle in Drosophila eggs and the persistence of chromosome individuality during interphase. This book is a valuable source of information for cytochemists, cell biologists, cell researchers, and protein nucleic acid enthusiasts who wish to expand their knowledge on comprehensive cytochemistry laboratory study.

List of Contributors

Preface

Acknowledgments

Contents of Volume II

Introduction

1. Submicroscopic Cytochemistry

Fixation

Staining

Other Preparatory Steps

Consideration of Artifacts of Freeze-Drying and Freeze-Substitution

Identification of Submicroscopic Structure in Cytochemical Electron Micrographs

Summary

References

2. Distribution of Protein in Hepatic and Exocrine Pancreatic Cells of the Mouse

Methods

Observations

Discussion

Summary

References

3. Methods for the Fixation and Staining of DNA and RNAs Applied to Exocrine Pancreatic and Hepatic Cells of the Mouse

Method

Observations

Discussion

Summary

Appendix

References

4. Cytochemical Study of Nucleic Acids in Salivary Gland Chromosomes of Drosophila Larvae

Materials and Methods

Observations

Discussion

Summary

References

5. Distribution of Protein in Nuclei of Salivary Glands of Drosophila Larvae

Methods

Observations

Discussion

Summary

References

6. DNA Pattern s of Cells of Imaginal Disks of Drosophila Larvae

Method

Discussion

Summary

References

7. Submicroscopic Distribution of Nucleic Acids in the Barr Body of Chondrocytes in the Proximal Tibial Epiphysis of Female Rats

Material and Methods

Observations

Discussion

Summary

References

8. Preliminary Study of the Specificity of the DNA Molecular Patterns of Differentiated Cells of the Young Adult Mouse

Method and Materials

General Observations

Discussion

Summary

References

9. Changing DNA Molecular Patterns during Cell Maturation in Method and Material

Observations

Discussion

Summary

References

10. Alteration of DNA Molecular Pattern s with Activation of Certain Cells in the Mouse

Methods

Observations

Discussion

Summary

References

11. DNA Molecular Pattern during Metaplasia of Stratified Squamous Epithelium of Mouse Vagina to Mucin-Producing Cells

Materials and Method

Observations

Discussion

Summary

References

12. Changes in Nuclear DNA Molecular Pattern of Spinal Ganglion Cells during Regeneration of Spinal Nerves of the Cat

Method

Observations

Discussion

Summary

References

13. Organization of DNA during the Mitotic Cycle in Eggs of Drosophila melanogaster

Methods

Observations

Discussion

Summary

References

14. Persistence of Individuality of Chromosomes during Interphase, and the Role of the Nuclear Membrane

Transformation of Interphase Chromatin to Prophase Chromosomes, and of Telophase Chromosomes to Interphase Chromatin, in Living Cells

Distribution of DNA in Chromatin in Unstimulated Somatic Cells

Distribution of DNA during the Change of Interphase Chromatin to Prophase

Chromosomes and of Telophase Chromosome to Interphase Chromatin

Changes in Distribution of DNA in Chromatin of Activated Cells

Changes in Distribution of DNA in Somatic Cell Nuclei during Differentiation and Maturation

Persistence of Specific Orientation of Chromosomes during Metaphase

Summary

References

Author Index

Subject Index