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Protein Biochemistry and Proteomics
- 1st Edition - February 21, 2006
- Imprint: Academic Press
- Author: Hubert Rehm
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 0 8 8 5 4 5 - 9
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 4 5 8 4 3 - 4
Hubert Rehm's Protein Biochemistry and Proteomics is more than a laboratory manual; it is a strategic guide that provides the reader with tips and tricks for more successful lab ex… Read more
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Hubert Rehm's Protein Biochemistry and Proteomics is more than a laboratory manual; it is a strategic guide that provides the reader with tips and tricks for more successful lab experiments. Using a conversational yet professional tone, Rehm provides an overview of a variety of methods in protein biochemistry/proteomics. He provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods. Readers will immediately sense that the author if very familiar with the challenges, and frustration of the daily lab routine. Never before has such an honest, tactical guide been available for those conducting lab experiments within the field of biochemistry.
- Shows how to avoid experimental dead ends and helps users develop an instinct for the right experiment at the right time
- Contains short and precise summaries of routine procedures (e.g. column chromatography, gel electrophoresis), and lists the advantages and disadvantages of alternative methods
- Includes over 100 detailed figures and tables
- Contains a chapter on proteomics
Laboratory practitioners such as students, instructors, and professionals, conducting experiments within the field of protein biochemistry.
The Concept of the Experimenter
Abbreviations
1. The Daily Bread
1.1 Making Buffers
1.2 Protein Determination
1.3 Gels
1.4 Staining Gels
1.5 Precipitate and Concentrating
1.6 Blotting
1.7 Autoradiography of Gels and Blots
2. Ligand Binding
2.1 Radioactive Ligand Marking
2.2 Binding
2.3 Analysis of Binding Data
2.4 Crosslinking of Ligands
2.5 Purposes
3. Solubilization of Membrane Proteins
3.1 Detergents
3.2 Solubilization
4. Protein Detection via Functional Measurements
4.1 Translocators
4.2 Reconstitution
4.3 Flux Assay
4.4 Constructive Thoughts
5. Cleaning and Purifying
5.1 Pure Fun
5.2 Conventional Purification Methods
5.3 Affinity Chromatography
5.4 The Purity Test
5.5 Profiting
6. Antibodies
6.1 Production of Polyclonal Antibodies
6.2 Immunoprecipitation
6.3 Immunoaffinity Chromatography
6.4 Antibodies Against Unpurified Proteins
6.5 Immunological detection techniques
7. Proteomics
7.1 Introduction
7.2 Sample taking
7.3 2D gel electrophoresis
7.4 Mass Spectroscopy of Peptides and Proteins
7.5 Protein Chips
7.6 Microsequencing
7.7 Strategy
8. Subunits
8.1 Number & Stoichiometry of Subunits
8.2 What Holds Our World Together
9. Glycoproteins
9.1 How, Where and for What Purpose are Proteins Glycosylated?
9.2 Detecting Glycoproteins in Gels
9.3 Detection of Glycoproteins on Blots
9.4 Deglycosylation
9.5 The Sugar Chains
10. Treasure Island
10.1 Of the Paper
10.2 Of Writing a Paper
11. Desert Planet
12. Jaws
12.1 Suppliers
12.2 Suppliers by Product
Last Things
Index
Abbreviations
1. The Daily Bread
1.1 Making Buffers
1.2 Protein Determination
1.3 Gels
1.4 Staining Gels
1.5 Precipitate and Concentrating
1.6 Blotting
1.7 Autoradiography of Gels and Blots
2. Ligand Binding
2.1 Radioactive Ligand Marking
2.2 Binding
2.3 Analysis of Binding Data
2.4 Crosslinking of Ligands
2.5 Purposes
3. Solubilization of Membrane Proteins
3.1 Detergents
3.2 Solubilization
4. Protein Detection via Functional Measurements
4.1 Translocators
4.2 Reconstitution
4.3 Flux Assay
4.4 Constructive Thoughts
5. Cleaning and Purifying
5.1 Pure Fun
5.2 Conventional Purification Methods
5.3 Affinity Chromatography
5.4 The Purity Test
5.5 Profiting
6. Antibodies
6.1 Production of Polyclonal Antibodies
6.2 Immunoprecipitation
6.3 Immunoaffinity Chromatography
6.4 Antibodies Against Unpurified Proteins
6.5 Immunological detection techniques
7. Proteomics
7.1 Introduction
7.2 Sample taking
7.3 2D gel electrophoresis
7.4 Mass Spectroscopy of Peptides and Proteins
7.5 Protein Chips
7.6 Microsequencing
7.7 Strategy
8. Subunits
8.1 Number & Stoichiometry of Subunits
8.2 What Holds Our World Together
9. Glycoproteins
9.1 How, Where and for What Purpose are Proteins Glycosylated?
9.2 Detecting Glycoproteins in Gels
9.3 Detection of Glycoproteins on Blots
9.4 Deglycosylation
9.5 The Sugar Chains
10. Treasure Island
10.1 Of the Paper
10.2 Of Writing a Paper
11. Desert Planet
12. Jaws
12.1 Suppliers
12.2 Suppliers by Product
Last Things
Index
- Edition: 1
- Published: February 21, 2006
- Imprint: Academic Press
- Language: English