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Principles of Genomics and Proteomics
- 1st Edition - January 25, 2023
- Authors: Rakeeb Ahmad Mir, Sheikh Mansoor Shafi, Sajad Majeed Zargar
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 0 4 5 - 5
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 9 3 7 8 - 4
Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproduci… Read more
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Request a sales quotePrinciples of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction.
A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments.
- Provides a comprehensive accounting of the technical aspects of genomic and proteomic analysis and applications in living organisms
- Includes detailed and standardized coverage of basic research methods in areas covered
- Presents advanced applications of genomics and proteomics that are highlighted with major significance in medical and agricultural fields
Graduate students in life science subjects like, Biotechnology, Genetics, Botany, Zoology, and Biochemistry, Established researchers in the field of Biotechnology, Genetics, Zoology, Botany and Microbiology
- Cover image
- Title page
- Table of Contents
- Copyright
- About the authors
- Foreword
- Foreword
- Preface
- Acknowledgments
- Chapter 1. Understanding the OMICS techniques: an introduction to genomics and proteomics
- 1. Introduction to genomics
- 2. Genomes of the eukaryotes—nuclear and organelle genomes
- 3. Organization of eukaryotic genome
- 4. Organelle genome editing
- 5. Introduction to proteomics
- 6. Proteome profiling
- 7. Conclusion
- Chapter 2. Genome mapping
- 1. Introduction
- 2. Markers
- 3. Physical mapping
- Chapter 3. Analysis of genomes—I
- 1. Introduction
- 2. Sangers sequencing/chain-termination sequencing method
- 3. Whole-genome shotgun sequencing
- 4. Hierarchical shotgun sequencing
- 5. Next-generation sequencing methods
- 6. Genome sequencing projects
- 7. Conclusion
- Chapter 4. Analysis of genomes—II
- 1. Introduction
- 2. DNA footprinting/deoxyribonuclease I (DNase I) protection mapping
- 3. Gel retardation assay or electrophoretic mobility shift assay or band shift assay
- 4. Chromatin immunoprecipitation and its variants
- 5. Conclusion
- Chapter 5. Nutrigenomics: Insights into the influence of nutrients on functional dynamics of genomes
- 1. Introduction
- 2. Nutrigenomics studies of selected crop plants—a repository of functional foods
- 3. Conclusion
- Chapter 6. Analysis of proteomes—I
- 1. Polyacrylamide gel electrophoresis and SDS-PAGE
- 2. Isoelectric focusing gels
- 3. 2D gel electrophoresis-combining SDS-PAGE and IEF for unison gel-based identification of proteins on the basis of size and charge
- 4. MALDI-TOF mass spectrometry
- 5. Isotope-coded affinity tags
- 6. Isotope-coded protein labeling
- 7. Isobaric tags for relative and absolute quantification
- 8. The tandem mass tag
- 9. Stable isotopic labeling of amino acids in cell culture
- 10. Discussion
- Chapter 7. Analysis of proteomes—II
- 1. Introduction
- 2. Ultraviolet and visible light spectroscopy
- 3. Fluorescence spectroscopy
- 4. Nuclear magnetic resonance spectroscopy
- 5. X-ray diffraction
- 6. Circular dichroism
- 7. Conclusion
- Chapter 8. Analysis of proteomes—III
- 1. Protein structure prediction
- 2. Protein secondary and tertiary structure predictions
- 3. Homology or template-based modeling
- 4. Protein threading
- 5. Ab initio protein structure predictions
- 6. Conclusions
- Chapter 9. Analysis of proteomes—IV
- 1. Introduction
- 2. Yeast 2-hybrid system
- 3. Principle
- 4. Advantages and limitations
- 5. Limitations of Y2H assays
- 6. Phage display
- 7. Application
- 8. Protein chips
- 9. Applications of protein microarrays
- 10. Conclusion and future prospective
- Chapter 10. Beyond genomics and proteomics
- 1. Introduction
- 2. Transcriptomics
- 3. Metabolomics
- 4. Interactomics
- 5. Lipidomics
- 6. Metagenomics
- 7. Ionomics
- 8. Connectomics
- 9. Conclusion
- Index
- No. of pages: 274
- Language: English
- Edition: 1
- Published: January 25, 2023
- Imprint: Elsevier
- Paperback ISBN: 9780323990455
- eBook ISBN: 9780323993784
RA
Rakeeb Ahmad Mir
Rakeeb Ahmad Mir is Assistant Professor, Department of Biotechnology, School of Life Sciences, Central University of Kashmir, Science Campus, Nunner, Ganderrbal, Srinagar, Jammu & Kashmir, India. He is having 6 years of Research and Teaching Experience in different subjects of Biotechnology. His research interests include Molecular taxonomy, Nematode genomics and basic research in proteomics. In addition, he is deeply involved in teaching courses such as, Cell Biology, Signal transduction and Cancer Biology, Molecular Biology, Genomics and Proteomics and Nematode genomics at University level. Dr Rakeeb has published various research and review papers, book chapters on Molecular taxonomy of nematodes, Proteomic techniques and stress tolerant crop plants. Since he has considerable experience in the related fields pertaining to the title of this book.
Affiliations and expertise
Department of Biotechnology, School of Biosciences & Biotechnology, Baba Ghulam Shah Badshah UniversitySS
Sheikh Mansoor Shafi
Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu. Dr. Mansoor has been dedicated to contributing significantly to his field and has displayed a strong commitment to becoming a highly accomplished scientist. His expertise spans various areas, including biochemistry, molecular biology, and genetics. Dr. Mansoor's accomplishments are notable, including over more than 50 research and review papers published in esteemed journals such as Frontiers, Plos One, Scientific Reports, Journal of Fungi (JoF), Planta, Chemosphere, JEMA and Molecules etc. He has also authored 5 books and more than ten book chapters, and holds credit for filing two patent applications. He has showcased his knowledge through guest lectures on topics like sequence assembly, alignment, submission tools, and phylogenetic analysis. He has received practical training in advanced techniques in modern biology and related fields. Dr. Mansoor has been recognized with numerous national and international accolades, including prestigious awards such as Best Thesis, Young Scientist, and recognition for his exceptional oral and poster presentations. He holds membership in a significant big grant (BIRAC) awarded by the Department of Biotechnology (DBT) in India. In addition to his accomplishments, Dr. Mansoor plays an active role as a Review Editor for Frontiers in Ethnopharmacology, and he also contributes as a reviewer for esteemed journals like 3 Biotech, Cell Reports, Frontiers, Plos One, and JARMAP. His unwavering dedication, profound expertise, and active involvement underscore his unwavering commitment to advancing scientific knowledge in his field.
Dr. Sheikh Mansoor currently serves as a
Postdoctoral Researcher at the Department
of Plant Resources and Environment and
Subtropical and Tropical Organism Gene
Bank within Jeju National University in
South Korea. Prior to this role, he held
positions as a Postdoc Research Associate at
SKIMS Srinagar and as a Project Associate-I
at CSIR-Indian Institute of Integrative Medicine
Jammu (IIIM). Throughout his academic
journey, Dr. Mansoor has had the
opportunity to work in reputed laboratories,
including the Transcriptomics Labs at
SKUAST Kashmir and the Department of
Biotechnology and Pathology at Central
Institute of Temperate Horticulture (CITH),
Rangreth, Srinagar. His educational background
comprises a BSc degree from SP
College of Science (University of Kashmir),
an MSc degree from HNBGU, Uttarakhand,
and a PhD degree from Sher-e-Kashmir
University of Agricultural Sciences and
Technology of Jammu. Dr. Mansoor has been
dedicated to contributing significantly to his
field and has displayed a strong commitment
to becoming a highly accomplished
scientist. His expertise spans various areas,
including biochemistry, molecular biology,
and genetics. Dr. Mansoor’s accomplishments
are notable, including more than 50
research and review papers published in
esteemed journals such as Frontiers, PLOS
One, Scientific Reports, Journal of Fungi (JoF),
Planta, Chemosphere, JEMA, Molecules etc. He
has also authored 5 books and more than 10
book chapters and holds credit for filing two
patent applications. He has showcased his
knowledge through guest lectures on topics
like sequence assembly, alignment, submission
tools, and phylogenetic analysis. He has
received practical training in advanced
techniques in modern biology and related
fields. Dr. Mansoor has been recognized
with numerous national and international
accolades, including prestigious awards such
as Best Thesis, Young Scientist, and recognition
for his exceptional oral and poster
presentations. He holds membership in a significant big grant (BIRAC) awarded by
the Department of Biotechnology (DBT) in
India. In addition to his accomplishments,
Dr. Mansoor plays an active role as a Review
Editor for Frontiers in Ethnopharmacology, and
he also contributes as a reviewer for
esteemed journals like 3 Biotech, Cell Reports,
Frontiers, PLOS One, and JARMAP. His
unwavering dedication, profound expertise,
and active involvement underscore his unwavering commitment to advancing scientific
knowledge in his field.
Affiliations and expertise
Postdoctoral Researcher, Phenomics Laboratory, Department of Plant Resources and Environment, Jeju National University, South KoreaSZ
Sajad Majeed Zargar
Sajad Majeed Zargar, Ph.D, is currently an Assistant Professor at Sher-e-Kashmir University of Sciences & Technology in India and visiting Professor at University of Padova, Italy. Dr. Zargar is the recipient of the CREST overseas fellowship from DBT, the Goho grant from Govt. of Japan, and the Erasmus Fellowship from European Union. He is also a member and representative of INPPO (International Plant Proteomics Organisation). His editorial activities and scientific memberships include publishing research and review articles in international journals and is also reviewer.
Affiliations and expertise
Proteomics Laboratory, Division of Biotechnology, Sher-e-Kashmir University of Sciences and Technology, J&K, India