Practical Approaches to Biological Inorganic Chemistry
- 1st Edition - January 15, 2013
- Editors: Robert R. Crichton, Ricardo O. Louro
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 4 - 5 6 3 5 1 - 4
- eBook ISBN:9 7 8 - 0 - 4 4 4 - 5 6 3 5 9 - 0
The book reviews the use of spectroscopic and related methods to investigate the complex structures and mechanisms of biological inorganic systems that contain metals. Each ch… Read more
The book reviews the use of spectroscopic and related methods to investigate the complex structures and mechanisms of biological inorganic systems that contain metals. Each chapter presents an overview of the technique including relevant theory, clearly explains what it is and how it works and then presents how the technique is actually used to evaluate biological structures. Practical examples and problems are included to illustrate each technique and to aid understanding. Designed for students and researchers who want to learn both the basics, and more advanced aspects of bioinorganic chemistry.
- Many colour illustrations enable easier visualization of molecular mechanisms and structures
- Worked examples and problems are included to illustrate and test the reader’s understanding of each technique
- Written by a multi-author team who use and teach the most important techniques used today to analyse complex biological structures
Postgraduates or postdocs plus chemical and biological researchers investigating biological structures
Preface
Chapter 1. An Overview of the Roles of Metals in Biological Systems
Introduction: Which Metals Ions and Why?
Some Physicochemical Considerations on Alkali Metals
Na+ and K+ – Functional Ionic Gradients
Mg2+ – Phosphate Metabolism
Ca2+ and Cell Signalling
Zinc – Lewis Acid and Gene Regulator
Iron and Copper – Dealing with Oxygen
Ni and Co – Evolutionary Relics
Mn – Water Splitting and Oxygen Generation
Mo and V – Nitrogen Fixation
References
Chapter 2. Introduction to Ligand Field Theory
Introduction
Crystal Field and Ligand Field Theory
MO Theory of Transition Metal Complexes
Concluding Remarks
Further Reading
Chapter 3. EPR Spectroscopy
Why EPR Spectroscopy?
What Is EPR Spectroscopy?
Anisotropy
A Comparison of EPR Versus NMR
EPR Spectrometer
What (Bio)molecules Give EPR?
Basic Theory and Simulation of EPR
Saturation
Concentration Determination
Hyperfine Interactions
High-Spin Systems
Applications Overview
References
Chapter 4. Introduction to Biomolecular NMR and Metals
Introduction
Properties of the Matter Relevant to NMR
Energy of NMR Transitions
Macroscopic Magnetisation
Acting on Magnetisation
Relaxation
An NMR Experiment
The Chemical Shift
Coupling: The Interaction Between Magnetic Nuclei
Chemical Exchange
The Nuclear Overhauser Effect
Multidimensional NMR
Metals in Biomolecular NMR Spectra
Relaxation
An NMR Spectrometer and How the Macroscopic Magnetisation and Relaxation are Measured
Care in Obtaining NMR Spectra of Paramagnetic Samples
Conclusions
References
Useful Physical Constants
Chapter 5. 57Fe-Mössbauer Spectroscopy and Basic Interpretation of Mössbauer Parameters
Introduction
Principles
57Fe Hyperfine Interactions
Isomer Shift as Informative Hyperfine Interaction
Electric Quadrupole Splitting
Magnetic Hyperfine Splitting
Combined Hyperfine Splitting
Applications – Selected Examples
Perspectives
References
Chapter 6. X-ray Absorption Spectroscopy in Biology (BioXAS)
Introduction to Biological X-Ray Absorption Spectroscopy (BioXAS)
An introductory example: Mo, Cu, AND Se in CO-dehydrogenase from Oligotropha carboxidovorans
Outline of the BioXAS Chapter
XANES
X-ray Absorption Spectroscopy: X-ray-induced Electron Diffraction
Phase Shifts and Effect of Atom Type
Plane-Wave and Muffin-Tin Approximation
Multiple Scattering in Biological Systems
Strategy for the Interpretation of EXAFS
Validation and Automation of EXAFS Data Analysis
XANES Simulations with three-dimensional Models
Metal–Metal Distances in Metal Clusters
Non-metal Trace Elements: Halogens
Summary: Strengths and Limitations
Conclusions: Relations with Other Techniques
References
Chapter 7. Other Spectroscopic Methods for Probing Metal Centres in Biological Systems
Optical Spectroscopy
Magnetic Circular Dichroism
Vibrational Spectroscopies
IR Spectroscopy
Raman Spectroscopy
References
Chapter 8. An Introduction to Electrochemical Methods for the Functional Analysis of Metalloproteins
Introduction
Basics
Electrochemistry Under Equilibrium Conditions: Potentiometric Titrations
Dynamic Electrochemistry
Diffusion-Controlled Voltammetry
Voltammetry of Adsorbed Proteins: Protein Film Voltammetry (PFV)
Catalytic Voltammetry and Chronoamperometry with Adsorbed Redox Enzymes
Softwares
PFV Quiz
Acknowledgements
Appendix
References
Chapter 9. X-ray Crystallography
Questions
Introduction
Protein Crystallisation
Data Collection
Phase Determination
Model Building and Refinement
Structure Analysis and Model Quality
Case Study
Introduction
Purification and Crystallisation
X-ray Diffraction Data Collection and Phasing
Structure Refinement
Structure Analysis
General Conclusions
Acknowledgements
References
Chapter 10. Genetic and Molecular Biological Approaches for the Study of Metals in Biology
Introduction and Aims
Basic Genetics and Molecular Genetics: Origins and Definitions
Setting Up: Regulations, Equipment, Methods and Resources
Approaches and Systems
Molecular Biology Tools and Methods
Genetic and Molecular Genetic Methods
Bioinformatics
The OMICS Revolution
Illustrative Examples in the Genetics and Molecular Biology of N2 Fixation
References
Index
- Edition: 1
- Published: January 15, 2013
- Language: English
RC
Robert R. Crichton
Dr. Robert R. Crichton is an Emeritus Professor, Faculty of Science, Universite Catholique de Louvain in the new town of Louvain-la-Neuve, Belgium, where he taught biochemistry to students of chemistry, biology and veterinary medicine and directed the Biochemistry Unit. He has published over two hundred scientific articles and since 1985 has organized over twenty Advanced Courses on Metals in Biology in Louvain-la-Neuve, training over 1300 doctoral and post-doctoral students, many of whom are today leaders in this field.
The Second and Third Editions of Biological Inorganic Chemistry: An Introduction to Molecular Structure and Function received the 2013 and 2019 TEXTY Textbook Excellence Awards in Physical Sciences respectively from the Text and Academic Authors Association.
Affiliations and expertise
Unite de Biochimie, Universite Catholique de Louvain, Louvain-la-Neuve, BelgiumRL
Ricardo O. Louro
Inorganic Biochemistry and NMR Laboratory ITQB-UNL Av da Republica (EAN), Portugal
Affiliations and expertise
Inorganic Biochemistry and NMR Laboratory ITQB-UNL Av da Republica (EAN), PortugalRead Practical Approaches to Biological Inorganic Chemistry on ScienceDirect