Polysaccharide Degrading Biocatalysts
- 1st Edition - February 15, 2023
- Editors: Rosana Goldbeck, Patricia Poletto
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 9 8 6 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 8 3 1 5 - 0
The transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis. Polysacch… Read more
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Request a sales quoteThe transformation of polysaccharides into valuable compounds for health and industry requires the careful application of enzyme protocols and controlled biocatalysis.
Polysaccharide-Degrading Biocatalysts
provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels, and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose-degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for the hydrolysis of polysaccharides, among other topics and related research protocols. A final chapter considers perspectives and challenges in an evolving, carbohydrate-based economy.- Describes the role of enzymes in the degradation of polysaccharides to obtain building blocks for biochemical processes
- Covers new tools for enzymatic evolution, research protocols, and process strategies contributing to large-scale applications
- Explores the use of polysaccharide hydrolysis products in the areas of pharmaceuticals, fuels, and food engineering
- Features chapter contributions from international experts
Basic and translational researchers in biochemistry, biotechnology, molecular biology, cellular biology, biomaterials, pathology, chemical biology, genetics, and pharmaceutical science, life science researchers, Vlinicians and graduate students in the biosciences
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Chapter 1: Plant cell wall polysaccharides: Methodologies for compositional, structural, and physicochemical characterization
- Abstract
- 1: Introduction to the analysis of plant cell wall polysaccharides
- 2: Sample preparation for the polysaccharides’ analysis
- 3: Chemical analysis of plant cell wall polysaccharides—Glycosyl residues composition
- 4: Structural analysis of plant cell wall polysaccharides
- 5: Complementary methods for the analysis of plant cell wall polysaccharides
- 6: Concluding remarks
- References
- Chapter 2: Genetic modification of plants to increase the saccharification of lignocellulose
- Abstract
- 1: Introduction
- 2: Lignin biosynthesis
- 3: Molecular approaches to cell wall modification
- 4: Technologies for genetic modification of the cell wall
- 5: Final considerations
- References
- Chapter 3: The diversity of plant carbohydrate hydrolysis in nature and technology
- Abstract
- Acknowledgments
- 1: Introduction
- 2: Types of hydrolysis
- 3: Plant sugars
- 4: Concluding remarks
- References
- Chapter 4: State-of-the-art experimental and computational approaches to investigate structure, substrate recognition, and catalytic mechanism of enzymes
- Abstract
- 1: Sample preparation for structural and biophysical analyses
- 2: Methods to analyze enzyme stability and structural homogeneity
- 3: Methods to analyze protein conformation and oligomerization
- 4: Methods to analyze enzyme-substrate interactions
- 5: Experimental approaches for structure elucidation
- 6: In silico methods for structural analysis
- References
- Chapter 5: Pretreatments as a key for enzymatic hydrolysis of lignocellulosic biomass
- Abstract
- 1: Introduction
- 2: Factors affecting enzymatic hydrolysis and their relationship with the pretreatment
- 3: Pretreatment of lignocellulosic biomass
- 4: Conclusions
- References
- Chapter 6: Importance of accessory enzymes in hemicellulose degradation
- Abstract
- Funding
- 1: Introduction
- 2: Xylanolytic enzymes
- 3: Mannanolytic enzymes
- 4: Conclusions
- References
- Chapter 7: How ligninolytic enzymes can help in the degradation of biomass polysaccharides, cleavage, and catalytic mechanisms?
- Abstract
- 1: Lignocellulosic biomass
- 2: Lignin
- 3: Ligninases
- 4: Ligninolytic enzymes and prospects
- References
- Chapter 8: Biochemical and biotechnological aspects of microbial amylases
- Abstract
- 1: Introduction
- 2: Amylase: The starch-digesting enzyme
- 3: Commercial production of α-amylases
- 4: Applications of α-amylase
- 5: Conclusion and future perspectives
- References
- Chapter 9: Hydrolysis of complex pectin structures: Biocatalysis and bioproducts
- Abstract
- Acknowledgment
- 1: Introduction
- 2: Pectin complex structure
- 3: Types of pectins
- 4: Sources of pectin
- 5: Pectin: Diverse uses
- 6: Pectinases
- 7: Structural aspects of protein families related to pectin degradation
- 8: Conclusion
- References
- Chapter 10: Macroalgal polysaccharides: Biocatalysts in biofuel/bioenergy production
- Abstract
- Acknowledgments
- 1: Introduction
- 2: The bio-refinery concept
- 3: Algae and its classification
- 4: Extraction of macroalgal polysaccharides
- 5: Biocatalysts in bio-refinery and biofuel production
- 6: Conclusions and future prospects
- References
- Chapter 11: Mathematical modeling of the enzymatic hydrolysis of polysaccharides: A primer
- Abstract
- 1: Aims and scope of this chapter
- 2: Scales at which the enzymatic hydrolysis of polysaccharides can be modeled
- 3: Features of substrates, enzymes, and models
- 4: The appropriate level of complexity for representing the system
- 5: General approaches to using deterministic models based on differential equations
- 6: General approaches to using stochastic models
- 7: “Fingerprinting models” as tools for estimating specificity constants
- 8: Conclusion
- References
- Chapter 12: Polysaccharide deconstruction products: Production of bio-based building blocks
- Abstract
- 1: Introduction
- 2: Succinic acid as a promising bio-based building block
- 3: Bio-based lactic acid: An important building block in biorefinery concept
- 4: Microbial propionic acid production
- 5: Conclusions
- References
- Chapter 13: Polysaccharide degradation for oligosaccharide production with nutraceutical potential for the food industry
- Abstract
- Acknowledgment
- 1: Introduction
- 2: Functional oligosaccharides
- 3: Sucrose-related oligosaccharides
- 4: Lactose-related oligosaccharides
- 5: Starch-related oligosaccharides
- 6: Nonstarch oligosaccharides
- 7: Algal-oligosaccharides
- 8: Concluding remarks
- References
- Chapter 14: Carbohydrate-active enzymes in the production of lactose-derived tagatose
- Abstract
- 1: d-Tagatose and production strategies of a rare sugar
- 2: β-Galactosidase and its applications
- 3: l-Arabinose isomerase
- 4: Integrated production of tagatose using immobilized enzymes
- References
- Chapter 15: Immobilized biocatalysts for hydrolysis of polysaccharides
- Abstract
- Graphical Abstract
- Acknowledgments
- 1: Introduction
- 2: Enzyme immobilization
- 3: Materials and techniques applied to hydrolysis of polysaccharides
- 4: Industrial applications
- 5: Final considerations
- References
- Chapter 16: Carbohydrate-based economy: Perspectives and challenges
- Abstract
- 1: Introduction
- 2: Market opportunities for carbohydrate-based products
- 3: What about the operational and environmental point of view?
- References
- Index
- No. of pages: 456
- Language: English
- Edition: 1
- Published: February 15, 2023
- Imprint: Academic Press
- Paperback ISBN: 9780323999861
- eBook ISBN: 9780323983150
RG
Rosana Goldbeck
Dr. Rosana Goldbeck is presently Associate Professor in the Food Engineering Faculty (FEA) at the University of Campinas (UNICAMP). She graduated in food engineering (2005) at the Federal University of Rio Grande (FURG), with a master’s degree (2008) and PhD (2012) in food engineering from the University of Campinas (UNICAMP), and a part of her PhD was carried out at the Universitat Autònoma de Barcelona (UAB). She held a postdoctoral degree at the Brazilian Laboratory of Bioethanol Science and Technology (CTBE) at the National Center for Research in Energy and Materials (CNPEM). Her experience is mainly in the area of biotechnology and bioengineering with emphasis in fermentative processes, production and purification of enzymes, cloning and expression of heterologous proteins, enzymatic hydrolysis, production of biofuels (bioethanol), and other value-added products (xylooligosaccharides) from agro-industrial residues.
Affiliations and expertise
Bioprocess and Metabolic Engineering Laboratory, School of Food Engineering, Department of Food Engineering and Technology, University of Campinas, Campinas, SP, BrazilPP
Patricia Poletto
Dr. Patrícia Poletto is Full Research Professor at the Federal University of Santa Catarina in Florianópolis—Brazil, and a collaborating researcher at the Biological Engineering Laboratory focused on fermentative and enzymatic processes. Patrícia completed her bachelor’s degree in food engineering (2007) and her PhD in biotechnology (2015) in the area of production and recovery of by solid-state fermentation. She has been working on chemical, fermentative, and enzymatic methods to convert agro-industrial residues mainly into oligosaccharides.
Affiliations and expertise
Laboratory of Biological Engineering, Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florianópolis, SC, BrazilRead Polysaccharide Degrading Biocatalysts on ScienceDirect