Plant Cell Biology
- 1st Edition, Volume 160 - August 31, 2020
- Imprint: Academic Press
- Editors: Ram Dixit, Elizabeth Haswell, Charles Anderson
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 5 3 3 - 3
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 1 5 3 4 - 0
Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant… Read more
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Request a sales quotePlant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis.
- Provide in-depth procedures and application notes from selected experts who developed the methods
- Each chapter will include figures and movies as appropriate to explain complex techniques
- Chapters will include caveats of techniques and future prospects
Cell biologists, biophysicists and physiologists who study plant cells and wish to expand their expertise to include new approaches
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Part I: Membrane and cytoplasm
- Chapter 1: Perturbation and imaging of exocytosis in plant cells
- Abstract
- 1: Introduction
- 2: Materials, reagents and equipment
- 3: Perturbing plant exocytosis using ES2
- 4: Using Brefeldin A to perturb protein trafficking
- 5: Analyzing CSC exocytosis dynamics using FRAP
- Acknowledgment
- Chapter 2: Electron tomography and immunogold labeling of plant cells
- Abstract
- 1: Introduction
- 2: Sample preparation
- 3: Image acquisition and analysis
- 4: Immunolabeling
- 5: Conclusions
- Acknowledgment
- Chapter 3: Methods for studying protein targeting to and within the chloroplast
- Abstract
- 1: Rationale
- 2: Synthesis of in vitro translated and radiolabeled substrate
- 3: Synthesis of recombinant substrate in E. coli
- 4: Chloroplast isolation
- 5: Chloroplast import assay
- 6: Recovery of intact chloroplasts through Percoll
- 7: Thermolysin and trypsin protease treatment
- 8: Subfractionation into soluble, alkaline-extracted, and alkaline-resistant proteins
- 9: Import-chase analysis
- 10: BN-PAGE analysis
- Chapter 4: Analyzing plant mechanosensitive ion channels expressed in giant E. coli spheroplasts by single-channel patch-clamp electrophysiology
- Abstract
- 1: Introduction
- 2: Methods
- Chapter 5: Tracking organelle movements in plant cells
- Abstract
- 1: Materials and equipment
- 2: Protocol
- 3: Analysis and statistics
- 4: Troubleshooting and optimization
- Acknowledgments
- Chapter 6: Evaluating molecular movement through plasmodesmata
- Abstract
- 1: Introduction
- 2: Techniques used to measure plasmodesmal permeability
- 3: DANS assays: Plant care, handling, dye preparation, and validation
- 4: DANS assays: Image acquisition, processing, and data analysis
- 5: A step-by-step guide to the DANS assay procedure
- Part II: Cell wall assembly, structure and mechanics
- Chapter 7: Unlocking the architecture of native plant cell walls via solid-state nuclear magnetic resonance
- Abstract
- 1: Introduction
- 2: Growing 13C-labeled plants
- 3: 13C Incorporation determination
- 4: Solid-state NMR analysis
- 5: Conclusions
- Acknowledgments
- Chapter 8: Heterologous expression of plant glycosyltransferases for biochemistry and structural biology
- Abstract
- 1: Introduction
- 2: Methods
- 3: Conclusions
- Acknowledgments
- Chapter 9: Studying cell wall mechanics using an automated confocal micro-extensometer
- Abstract
- 1: Rationale
- 2: Materials, equipment and reagents
- 3: Protocols
- 4: Summary
- Chapter 10: Staining and automated image quantification of callose in Arabidopsis cotyledons and leaves
- Abstract
- 1: Introduction
- 2: Elicitation
- 3: Aniline blue staining, tissue mounting and microscopy
- 4: Quantification of callose deposits
- 5: Precursor techniques
- 6: Conclusion
- Acknowledgments
- Chapter 11: Imaging the delivery and behavior of cellulose synthases in Arabidopsis thaliana using confocal microscopy
- Abstract
- 1: Introduction
- 2: Collecting time-lapses of cellulose synthase particles
- 3: Using FRAP to analyze newly delivered cellulose synthase particles
- 4: Precursor techniques
- 5: Safety considerations and standards
- 6: Pros and cons
- 7: Alternative methods/procedures
- 8: Troubleshooting and optimization
- 9: Conclusion
- Acknowledgments
- Chapter 12: Partial purification and immunodetection of cell surface glycoproteins from plants
- Abstract
- 1: Introduction/rationale
- 2: Materials, reagents and equipment
- 3: Methods
- 4: Microsome isolation
- 5: Enrichment of arabinogalactan glycoproteins
- 6: Partial purification by immobilized metal affinity chromatography (IMAC)
- 7: Immunodetection of glycosylation
- 8: Conclusions
- Acknowledgments
- Part III: Cytoskeleton dynamics and organization
- Chapter 13: Super-resolution imaging of microtubules in Medicago sativa
- Abstract
- 1: Introduction
- 2: Materials
- 3: Sample preparation
- 4: Super-resolution microscopy methods for microtubule imaging in M. sativa
- 5: Conclusions
- Acknowledgment
- Chapter 14: Live imaging of microtubules in petal conical cells
- Abstract
- 1: Introduction
- 2: Live-cell confocal imaging of geometric shape of conical cells
- 3: Live-cell imaging of cortical microtubules in conical cells
- 4: Conclusions
- Acknowledgments
- Chapter 15: Affinity purification of tubulin from plant materials
- Abstract
- 1: Introduction
- 2: Bacterial expression and purification of GST-TOG protein
- 3: Preparation of a TOG column
- 4: Purification of endogenous tubulin
- 5: Purification of His-tagged tubulin from plant cells
- 6: Pelleting assay
- 7: Trouble shooting and optimization
- 8: Summary
- Acknowledgment
- Chapter 16: Quantifying the polymerization dynamics of plant cortical microtubules using kymograph analysis
- Abstract
- 1: Introduction
- 2: Requirements
- 3: Methods
- 4: Concluding remarks
- Acknowledgments
- Part IV: Cell growth and morphology
- Chapter 17: Simultaneous measurement of turgor pressure and cell wall elasticity in growing pollen tubes
- Abstract
- 1: Introduction
- 2: Method
- Chapter 18: 3D morphological analysis of Arabidopsis sepals
- Abstract
- 1: Introduction
- 2: 3D morphometry of the sepal by CSLM and MGX analysis
- 3: Conclusions
- Acknowledgment
- Chapter 19: Assembly of a simple scalable device for micromechanical testing of plant tissues
- Abstract
- 1: Introduction
- 2: Rationale
- 3: Device presentation
- 4: Requirements
- 5: Device assembly
- 6: Device calibration
- 7: Example of tensile tests on plant tissue using onion epidermis
- 8: Considerations and possible improvements
- Acknowledgments
- Chapter 20: User-friendly assessment of pavement cell shape features with PaCeQuant: Novel functions and tools
- Abstract
- 1: Introduction
- 2: Cell segmentation and feature extraction with PaCeQuant
- 3: Manual postprocessing of label images with the LabelImageEditor
- 4: Feature visualization in segmented cells with the FeatureColorMapper
- 5: Statistical analysis and data visualization with PaCeQuantAna in R
- Acknowledgments
- Chapter 21: Long-term live-cell imaging techniques for visualizing pavement cell morphogenesis
- Abstract
- 1: Introduction
- 2: Materials, equipment, and reagents
- 3: Methods
- Chapter 22: Microfluidic systems for plant root imaging
- Abstract
- 1: Introduction
- 2: Microdevice design
- 3: Microdevice fabrication
- 4: RootChip-8S preparation for seedling cultivation
- 5: Imaging roots grown in the RootChip-8S
- 6: Trouble-shooting
- Acknowledgments
- Chapter 23: MAGIC: Live imaging of cellular division in plant seedlings using lightsheet microscopy
- Abstract
- 1: Introduction
- 2: Definition
- 3: Materials, equipment and reagents
- 4: Protocols
- 5: Pros and cons
- 6: Troubleshooting and optimization
- 7: Summary
- Acknowledgments
- Chapter 24: BioVision Tracker: A semi-automated image analysis software for spatiotemporal gene expression tracking in Arabidopsis thaliana
- Abstract
- 1: Introduction
- 2: Equipment and materials
- 3: Protocol
- 4: Summary
- Acknowledgments
- Edition: 1
- Volume: 160
- Published: August 31, 2020
- No. of pages (Hardback): 456
- No. of pages (eBook): 456
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780128215333
- eBook ISBN: 9780128215340
RD
Ram Dixit
Ram Dixit obtained a PhD in Plant Molecular Biology at Cornell University in 2000, working on mechanisms of cell-cell recognition. He conducted postdoctoral research at Penn State University (2000-2005) on the dynamics and organization of plant cortical microtubules and later at the University of Pennsylvania (2005-2008) on molecular mechanisms regulating motor proteins and structural microtubule-associated proteins. In 2008, he started as an Assistant Professor in the Biology Department at Washington University in St. Louis, where he is currently Associate Professor. His research group seeks to understand the molecular mechanisms of microtubule array assembly and cell wall deposition that determine plant cell morphogenesis. He is Associate Director of Education for the NSF Science and Technology Center for Engineering Mechanobiology and is a Guest Editor for The Plant Cell.
Affiliations and expertise
Associate Professor, Biology Department, Washington University in St. Louis, USAEH
Elizabeth Haswell
Elizabeth Haswell earned her PhD in Biochemistry at the University of California, San Francisco in 2000, working on chromatin remodeling in yeast. She then switched to plant systems for her postdoctoral work as a Life Sciences Research Fellow at Caltech, where she fell in love with plants and mechanobiology. In 2007, she joined the faculty in the Biology department at Washington University in Saint Louis, where she is now a Professor of Biology and HHMI-Simons Faculty Scholar. Her group there aims to identify the molecular and cellular mechanisms by which plants perceive force, with a particular focus on mechanosensitive ion channels. Liz is a Senior Editor for The Plant Cell and Director of Research for the NSF Science and Technology Center for Engineering Mechanobiology. She serves on the North American Arabidopsis Steering Committee and the Multinational Arabidopsis Steering Committee and is an AAAS Council Delegate. She is an advocate for science communication and for an academic culture that values sustainability, diversity, and authenticity. She is also a co-host of the Plantae podcast, The Taproot.
Affiliations and expertise
Professor of Biology and HHMI-Simons Faculty Scholar, Washington University in St. Louis, USACA
Charles Anderson
Charles T. Anderson completed a PhD at Stanford University in 2008, studying the biogenesis and function of primary cilia in mammalian cells. For his post-doctoral research as part of the Energy Biosciences Institute at the University of California Berkeley, he began working on the expansion and biosynthesis of plant cell walls. He accepted a position as an Assistant Professor of Biology at Penn State University in 2011 and was promoted to Associate Professor in 2018. His research group seeks to understand how the synthesis, trafficking, assembly, and remodeling of cell wall components influence cell wall structure and cell growth in plants, and to identify and characterize new genes that influence the dynamics of plant cell walls. His research aims to inform efforts to use plant cell walls to provide sustainable food, materials, and energy for human society. He is a Reviewing Editor at The Plant Cell, co-directs the Center for Biorenewables, and is the Sustainability Chair for the Eberly College of Science at Penn State.
Affiliations and expertise
Associate Professo of Biology, Penn State University, USARead Plant Cell Biology on ScienceDirect