Physical Principles and Techniques of Protein Chemistry Part B
- 1st Edition - January 1, 1970
- Latest edition
- Editor: Sydney Leach
- Language: English
Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is… Read more
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Description
Description
Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.
Table of contents
Table of contents
List of Contributors
Preface
Contents of Other parts
10. Ultracentrifugal Analysis
Glossary of Symbols
I. Introduction
II. Fundamentals of the Method
III. Subsidiary Measurements Required in Ultracentrifugal Analysis
IV. Sedimentation Velocity Behavior of Noninteracting Solutes
V. Sedimentation Equilibrium of Noninteracting Solutes
VI. The Archibald Method. Equilibrium at the Menisci Only
VII. Sedimentation Behavior of Interacting Solutes
VIII. Zonal Analytical Velocity Sedimentation
IX. Isopycnic Density Gradient Sedimentation
X. A Brief Comparison of Some Commercially Available Ultracentrifuges
References
11. Viscosity
Glossary of Symbols
I. Introduction
II. Viscosity and Intrinsic Viscosity
III. Experimental Methods
IV. Theories for "Model" Particles of Various Shapes
V. Dependence of Viscosity or Intrinsic Viscosity on Various Factors
VI. Use of Viscosity Measurements in Protein Chemistry
References
12. Light Scattering
Glossary of Symbols
I. Introduction
II. Principles
III. Practice
Appendix
References
13. Infrared Methods
Glossary of Symbols
I. Introduction
II. Measurement of Spectra
III. Quantitative Analysis of Spectra
IV. Correlation of Spectra with Structure
References
14. Nuclear Magnetic Resonance Spectroscopy
Glossary of Symbols
I. Introduction
II. Basic Theory of Nuclear Magnetic Resonance
III. Experimental Techniques
IV. Applications
References
15. Binding of Protons and Other Ions
Glossary of Symbols
I. Introduction
II. General Considerations in Ion Binding
III. Specificity of Binding Environments Illustrated for Hydrogen Ions
IV. Ion Binding in the Crystalline State
V. Ion Binding by Native Proteins in Solution
VI. Major Conformational Changes Related to Ion Binding
VII. Some Problems for Future Study
References
16. Differential Thermal Analysis
I. Introduction
II. The Basic Principles
III. Presentation of Data
IV. Instrumentation and Methodology
V. Limitations
VI. Applications to Polymers
VII. Protein Analyses
VIII. Future Applications
IX. Complementary Methods
References
Author Index
Subject Index
Product details
Product details
- Edition: 1
- Latest edition
- Published: January 1, 1970
- Language: English
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