Peptide and Peptide Mimicking Tools: Methods, Synthesis, Design & Applications
- 1st Edition, Volume 698 - June 18, 2024
- Editor: David M. Chenoweth
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 2 1 8 1 4 - 9
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 1 8 1 5 - 6
Peptide Chemical Tools for Modulating Biology, Volume 698 in the esteemed Methods in Enzymology series, highlights new advances in the field, with this new volume presenting in… Read more
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Request a sales quotePeptide Chemical Tools for Modulating Biology, Volume 698 in the esteemed Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting topics on Peptide tools that target telomere maintenance, Molecular Design of Peptide Therapeutics, Sulfonyl peptide tools for modulating biology, Peptide tools for targeting the Crk/CrkL-p130Cas axis, Quorum sensing peptide tools, In vivo stability and BBB penetration of peptide tools, and Oligo-benzamide-based peptide mimicking tools for modulating biology.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology
- Updated release includes the latest information on Peptide Chemical Tools for Modulating Biology
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- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Chapter One: Synthesis, derivatization, and conformational scanning of peptides containing N-Aminoglycine
- Abstract
- 1 Introduction
- 2 Protocols for the synthesis of aGly-containing peptides
- 3 Tips and troubleshooting
- 4 Applications to conformational scanning
- 5 Summary
- Acknowledgements
- References
- Chapter Two: Thioimidates provide general access to thioamide, amidine, and imidazolone peptide-bond isosteres
- Abstract
- 1 Introduction
- 2 Thioamide peptide-bond isosteres
- 3 Amidine peptide-bond isosteres
- 4 (4H)-Imidazolone peptide-bond isosteres
- 5 Materials
- 6 Protocol for synthesis of protected thionopeptides
- 7 Protocol for cleavage & deprotection of protected thionopeptides
- 8 Protocol for amidinopeptide synthesis via thioimidates
- 9 Protocol for backbone imidazolone peptide synthesis via thioimidates
- 10 Summary and conclusions
- Acknowledgements
- References
- Chapter Three: A single backbone amide modification method to achieve single site-specific chemical protein conjugation
- Abstract
- 1 Introduction
- 2 Developing CAST for a single peptide backbone amide modification
- 3 Protein labeling using CAST
- 4 Antibody modification using CAST
- 5 Developing plasma-stable CASTi
- 6 Tra-CASTi-MMAE preparation
- 7 Antitumor activity evaluation of Tra-CASTi-MMAE in vitro and in vivo
- 8 Summary and conclusions
- Acknowledgments
- References
- Chapter Four: Chemical cyclization of tyrosine-containing peptides via in situ generated triazolinedione peptides
- Abstract
- 1 Introduction
- 2 Chemoselective cyclization of tyrosine-containing peptides
- 3 Protocol
- 4 Summary and conclusions
- Acknowledgements
- References
- Chapter Five: Highly selective chemical modification of poly-His tagged peptides and proteins
- Abstract
- Abbreviations
- 1 Introduction
- 2 Safety considerations
- 3 Gram scale synthesis of the azidoacetate ester
- 4 Synthesis of the biotin functionalized ester
- 5 Recombinant protein expression
- 6 Preparation of stock solutions
- 7 Peptide and protein modifications
- 8 Summary
- Acknowledgments
- References
- Chapter Six: Tetrazine cyclized peptides for one-bead-one-compound library: Synthesis and sequencing
- Abstract
- 1 Introduction
- 2 Protocols
- 3 Optimization and troubleshooting
- 4 Future directions
- Acknowledgements
- References
- Chapter Seven: One-pot ligation of multiple peptide segments via N-terminal thiazolidine deprotection chemistry
- Abstract
- 1 Introduction
- 2 C-to-N one-pot ligation of multiple peptide segments
- 3 General protocol for peptide segment synthesis
- 4 General protocol for one-pot peptide ligation with repetitive Thz deprotection by 2-aminobenzamide type aldehyde scavenger
- 5 Summary
- Acknowledgement
- References
- Chapter Eight: Molecular design of peptide therapeutics via N-terminal modification
- Abstract
- 1 Introduction
- 2 N-terminal alkylation using trifluoroethyl iodonium salts
- 3 N-terminal alkylation by reductive amination
- 4 Luciferase based cell assay
- 5 Summary
- References
- Chapter Nine: Oligo-benzamide-based peptide mimicking tools for modulating biology
- Abstract
- 1 Introduction
- 2 Design and application of oligo-benzamides as α-helix mimetics
- 3 Synthetic strategies for producing oligo-benzamides
- 4 Materials
- 5 Reagents
- 6 Synthetic procedural details
- 7 Summary
- Acknowledgements
- References
- Chapter Ten: Sulfonyl γ-AApeptide tools for modulating biology
- Abstract
- 1 Introduction
- 2 Crystal structures and rational design
- 3 Synthesis of sulfonyl-γ-AApeptides
- 4 Biomedical applications
- 5 Summary and future directions
- Acknowledgements
- References
- Chapter Eleven: Designing and synthesizing peptide-based quorum sensing modulators
- Abstract
- Abbreviations
- 1 Introduction
- 2 General methodology and design strategy
- 3 Synthesis of linear quorum sensing peptides
- 4 Purification & analysis of linear quorum sensing peptides
- 5 Synthesis of cyclic quorum sensing peptides
- 6 Conclusion
- Acknowledgement
- References
- Chapter Twelve: Use of phosphotyrosine-containing peptides to target SH2 domains: Antagonist peptides of the Crk/CrkL-p130Cas axis
- Abstract
- 1 Introduction
- 2 Peptide design and synthesis
- 3 Protein expression and purification
- 4 Modeling of peptide–protein complexes
- 5 Characterization of peptide-protein interaction
- 6 Modulation of protein-protein interactions by peptides (GST pulldown)
- 7 Outlook
- Acknowledgments
- References
- Chapter Thirteen: Design and synthesis of oxytocin glycosides for the treatment of pain and substance use disorder
- Abstract
- 1 Introduction
- 2 Materials
- 3 Equipment
- 4 Synthesis of serine lactoside (SerLact)
- 5 Synthesis of the oxytocin lactoside analogue
- 6 Discussion
- 7 Conclusion
- References
- Chapter Fourteen: Fluorescence polarization assay for screening FANCM-RMI inhibitors to target the alternative lengthening of telomeres
- Abstract
- 1 Introduction
- 2 Expression and purification of RMI1-RMI2 heterodimer
- 3 TAMRA fluorescent tracer
- 4 RMI1-RMI2 fluorescence polarization assay
- 5 Conclusions
- Acknowledgments
- References
- No. of pages: 412
- Language: English
- Edition: 1
- Volume: 698
- Published: June 18, 2024
- Imprint: Academic Press
- Hardback ISBN: 9780443218149
- eBook ISBN: 9780443218156
DC
David M. Chenoweth
David Chenoweth is at University of Pennsylvania, PA, USA
Affiliations and expertise
University of Pennsylvania, PA, USARead Peptide and Peptide Mimicking Tools: Methods, Synthesis, Design & Applications on ScienceDirect