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A Guide to Methods and Applications
1st Edition - December 28, 1989
Editors: Michael A. Innis, David H. Gelfand, John J. Sninsky, Thomas J. White
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The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive… Read more
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The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
Avoid contamination--with specific instructions on setting up your lab
Avoid cumbersome molecular biological techniques
Discover new applications
Researchers and technicians in molecular biology, in both basic research and applications, including genetics and disease diagnosis
[Contents in a condensed version] Basic Methodology Amplification of genomic DNA targets Optimization of PCR reaction and design of primers Nonradioactive detection of PCR products mRNA quantitation by PCR Cloning PCR products Contamination and product carry-over PCR from paraffin sections Amplifying ancient DNA Research Applications Direct DNA sequencing of PCR products DNA footprinting Detection of rare mRNAs Site-directed mutagenesis Screening DNA libraries Amplification of flanking DNA sequences Genetics and Evoltion Tissue typing and disease susceptibility Haplotype analysis of single cells Population biology Multiplex DNA amplification Diagnostics and Forensics HIV detection HTLV I and II detection Screening for novel viruses Detection of oncogenic mutations Diagnosis of retinoblastoma Individual identification Instruments and Supplies Organizing a laboratory for PCR work Low-cost air-driven cycling oven Basic equipment and supplies for the PCR lab