PCR Protocols
A Guide to Methods and Applications
- 1st Edition - December 28, 1989
- Latest edition
- Editors: Michael A. Innis, David H. Gelfand, John J. Sninsky, Thomas J. White
- Language: English
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive… Read more
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
- Avoid contamination--with specific instructions on setting up your lab
- Avoid cumbersome molecular biological techniques
- Discover new applications
Researchers and technicians in molecular biology, in both basic research and applications, including genetics and disease diagnosis
[Contents in a condensed version]
Basic Methodology
Amplification of genomic DNA targets
Optimization of PCR reaction and design of primers
Nonradioactive detection of PCR products
mRNA quantitation by PCR
Cloning PCR products
Contamination and product carry-over
PCR from paraffin sections
Amplifying ancient DNA
Research Applications
Direct DNA sequencing of PCR products
DNA footprinting
Detection of rare mRNAs
Site-directed mutagenesis
Screening DNA libraries
Amplification of flanking DNA sequences
Genetics and Evoltion
Tissue typing and disease susceptibility
Haplotype analysis of single cells
Population biology
Multiplex DNA amplification
Diagnostics and Forensics
HIV detection
HTLV I and II detection
Screening for novel viruses
Detection of oncogenic mutations
Diagnosis of retinoblastoma
Individual identification
Instruments and Supplies
Organizing a laboratory for PCR work
Low-cost air-driven cycling oven
Basic equipment and supplies for the PCR lab
Basic Methodology
Amplification of genomic DNA targets
Optimization of PCR reaction and design of primers
Nonradioactive detection of PCR products
mRNA quantitation by PCR
Cloning PCR products
Contamination and product carry-over
PCR from paraffin sections
Amplifying ancient DNA
Research Applications
Direct DNA sequencing of PCR products
DNA footprinting
Detection of rare mRNAs
Site-directed mutagenesis
Screening DNA libraries
Amplification of flanking DNA sequences
Genetics and Evoltion
Tissue typing and disease susceptibility
Haplotype analysis of single cells
Population biology
Multiplex DNA amplification
Diagnostics and Forensics
HIV detection
HTLV I and II detection
Screening for novel viruses
Detection of oncogenic mutations
Diagnosis of retinoblastoma
Individual identification
Instruments and Supplies
Organizing a laboratory for PCR work
Low-cost air-driven cycling oven
Basic equipment and supplies for the PCR lab
- Edition: 1
- Latest edition
- Published: December 28, 1989
- Language: English
MI
Michael A. Innis
Affiliations and expertise
Cetus Corporation, Emeryville, California, U.S.A.DG
David H. Gelfand
Affiliations and expertise
Roche Molecular Systems, Alameda, California, U.S.A.JS
John J. Sninsky
Affiliations and expertise
Roche Molecular Systems, Alameda, California, U.S.A.TW
Thomas J. White
Affiliations and expertise
Hoffmann-Roche Diagnostic Research, Almeda, California, U.S.A.Read PCR Protocols on ScienceDirect