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An Ultimate Benchtop Reference for Molecular Biologists
1st Edition - November 28, 2016
Authors: Ayaz Najafov, Gerta Hoxhaj
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PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging… Read more
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PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR.
Provides troubleshooting tips for various types of modifications of standard protocols
Contains unique “Good Practices and Tips” that are indispensable for the beginner and expert alike
Features “Special Cases” with applications of PCR, optimization, and troubleshooting
Includes detailed appendices with tables, figures, and key protocols
Organized as a systematic, concentrated resource to save time when addressing a PCR problem
Researchers and students in molecular biology, biochemistry, cell biology, genetics, and cancer biology
Appendix E: Protocol for DNA Concentration Estimation Using Hoechst 33258
Appendix F: Formulas
Appendix G: Common Primers for Sequencing, Promoters, Housekeeping Genes, and Reporters
Appendix H: Tags
Appendix I: Thermostable DNA polymerases
Appendix J: Example of a Daily PCR Log
Appendix K: Common Buffers and Solutions
Appendix L: Useful Numbers
Appendix M: Yeast Colony PCR
No. of pages: 104
Published: November 28, 2016
Imprint: Academic Press
Paperback ISBN: 9780128042311
eBook ISBN: 9780128042465
Ayaz was born in Baku, Azerbaijan. He obtained his BSc and MSc degrees in Molecular Biology and Genetics from Bosphorus University, Istanbul, Turkey and got a 2-year training in Biochemistry as an intern at Baylor College of Medicine, Houston, Texas, USA. Ayaz pursued his PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Dario Alessi’s lab. Ayaz is currently a postdoctoral fellow in Prof. Junying Yuan’s lab at Harvard Medical School, Cell Biology Department.
Affiliations and expertise
Junying Yuan Lab, Department of Cell Biology, Harvard Medical School, USA
Gerta was born in Fier, Albania. She obtained her BSc degrees in Molecular Biology and Genetics, as well as a second major in Chemistry from Bosphorus University, Istanbul, Turkey. Gerta pursued her PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Carol MacKintosh’s lab. Gerta is currently a postdoctoral fellow in Prof. Brendan Mannings’s lab at Harvard T.H. Chan School of Public Health, Department of Genetics and Complex Diseases.
Affiliations and expertise
Department of Genetics and Complex Diseases, Harvard T.H. Chan School of Public Health, USA