
Oxidants and Antioxidants, Part A
- 1st Edition, Volume 299 - October 2, 1998
- Imprint: Academic Press
- Editors: John N. Abelson, Helmut Sies, Melvin I. Simon
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 3 9 1 7 8 9 - 8
- Hardback ISBN:9 7 8 - 0 - 1 2 - 1 8 2 2 0 0 - 2
- eBook ISBN:9 7 8 - 0 - 0 8 - 0 8 8 4 1 0 - 3
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since… Read more

Purchase options

Institutional subscription on ScienceDirect
Request a sales quoteThe critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
- Total Antioxidant Activity
- Vitamin C
- Polyphenols and Flavanoids
- Thiols
- Vitamin E and Coenzyme Q10
- Carotenoids and Retinoids
Researchers in biochemistry, pharmacology, physiology, cell biology, molecular biology, and biomedicine
Total Antioxidant Activity:
H. Alho and J. Leinonen, Total Antioxidant Activity Measured by Chemiluminescence Methods.
I.F.F. Benzie and J.J. Strain, Ferric Reducing/Antioxidant Power Assay: Direct Measure of "Total Antioxidant Activity" of Biological Fluids, and a Modified Version (FRASC) Which Enables the Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration.
Y. Noda, M. Kohno, A. Mori, and L. Packer, Automated ESR Free Radical Detector Assays for Antioxidant Activity in Natural Extracts.
A. Kontush and U. Beisiegel, Measurement of Oxidizability of Blood Plasma.
G. Cao and R.L. Prior, Measurement of Oxygen Radical Absorbance Capacity in Biological Samples.
Vitamin C:
M. Levine, Y. Wang, and S.C. Rumsey, Analysis of Ascorbic and Dehydroascorbic Acid in Biological Samples.
A.M. Bode and R.C. Rose, Analysis of Water-Soluble Antioxidants by High-Performance Liquid Chromatography with Electrochemical Detection.
J. Lykkesfeldt and B.N. Ames, Ascorbic Acid Recycling in Rat Hepatocytes as a Measurement of Antioxidant Capacity: Decline with Age.
Polyphenols and Flavonoids:
L.C. Bourne and C. Rice-Evans, Method for Detecting and Measuring Bioavailability of Phenolics and Flavonoids in Humans: Pharmacokinetics of Urinary Excretion of Dietary Ferulic Acid.
T. Goto and Y. Yoshida, Simultaneous Analysis of Individual Catechins and Caffeine in Green Tea.
A.L. Waterhouse, S.F. Price, and J.D. McCord, Reverse Phase HPLC Methods for Analysis of Wine Polyphenols.
D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by High-Performance Liquid Chromatography.
D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by Gas Chromatography-Mass Spectrometry.
V.L. Singleton, R. Orthofer, and R.M. Lamuela-Raventós, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent.
V. Cheynier, J.-M. Souquet, E. LeRoux, S. Guyot, and J. Riguad, Size Separation of Condensed Tannins by Normal-Phase HPLC.
R.M. Lamuela-Raventós and A. Waterhouse, Resveratrol and Piceid in Wine.
E.N. Frankel, Antioxidant Activity by Headspace Gas Chromatography of Volatile Oxidation Products of w-6 and w-3 p Polyunsaturated Lipids.
P.C.H. Hollman, D.P. Venema, S. Khokhar, and I.C.W. Arts, Determination of Tea Catechins by Reversed Phase HPLC.
A.S. Pannala, S. Singh, and C. Rice-Evans, Flavonoids as Peroxynitrite Scavengers in Vitro.
Thiols:
C.K. Sen, S. Roy, S. Khanna, and L. Packer, Determination of Oxidized and Reduced Lipoic Acid Using High Performance Liquid Chromatography and Coulometric Detection.
C.K. Sen, S. Roy, and L. Packer, Flow Cytometric Determination of Cellular Thiols.
L.A. Ridnour, R.A. Winters, N. Ercal, and D.R. Spitz, Measurement of Glutathione, Glutathione Disulfide and Other Thiols in Mammalian Cell and Tissue Homogenates Using HPLC Separation of N-(1n-pyrenyl)maleimide Derivatives.
M. Asensi, J. Sastre, F.V. Pallardo, A. Lloret, M. Lehner, J. Garcia-De-La Asuncion, and J. Viña, GSH:GSSG Ratio as an Indicator of Oxidative Stress Status and DNA Damage.
J. Chaudiere, N. Aguini, and J.-C. Yadan, Nonenzymatic Colorimetric Assay of Glutathione in the Presence of Other Mercaptans.
H. Baker, B. DeAngelis, E.R. Baker, and S.H. Hutner, Protozoological Method for Assaying Lipoate in Human Biologic Fluids and Tissue.
O. Tirosh, Y. Katzhendler, Y. Barenholz, and R. Kohen, Antioxidant Activity of Amidothionophosphates.
K. Traber and L. Packer, Quantitation of Anethole Dithiolthione Using High Pressure Liquid Chromatography with Electrochemical Detection. Vitamin E and Coenzyme Q10:
D.C. Liebler, J.A. Burr, and A.J.L. Ham, Gas Chromatography-Mass Spectrometry Analysis of Vitamin E and Its Oxidation Products.
J.K.G. Kramer, R.C. Fouchard, and K.M.R. Kallury, Improved Method to Determine Vitamin E Forms in Tissues and Diets by High Performance Liquid Chromatography Using a Normal-Phase Diol Column.
M. Podda, C. Weber, M.G. Traber, R. Milbradt, and L. Packer, Sensitive HPLC Techniques for Simultaneous Determination of Tocopherols, Tocotrienols, Ubiquinols, and Ubiquinones in Biological Samples.
B. Finckh, A. Kontush, J. Commentz, C. Hübner, M. Burdelski, and A. Kohlschütter, HPLC Analysis of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma--Coulometric Electrochemical Detection.
C.-P. Aebischer, J. Schierle, and W. Schüep, Simultaneous Determination of Retinol, Tocopherols, Carotene Lycopene and Xanthophylls in Plasma by Means of Reversed Phase HPLC.
P.K. Witting, D. Mohr, and R. Stocker, Assessment of the Prooxidant Activity of Vitamin E in Human Low Density Lipoprotein and Plasma.
Carotenoids and Retinoids:
N. Pellegrini, R. Re, M. Yang, and C.A. Rice-Evans, Screening of Dietary Carotenoids and Carotenoid-Rich Fruit Extracts for Antioxidant Activities Applying the ABTs Radical Cation Decolorization Assay.
T. Wingerath, D. Kirsch, R. Kaufmann, W. Stahl, and H. Sies, Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry (MALDI-PSD-MS) in Carotenoid Analysis.
A. Mortensen and L.H. Skibsted, Carotenoid Photobleaching.
M.A. Livrea and L. Tesoriere, Interactions Between Vitamin A and Vitamin E in Liposomes and in Biological Contexts.
T.E. Gundersen and R. Blomhoff, On-Line Solid Phase Extraction and Isocratic Separation of Retinoic Acid Isomers in a Micro-bore Column Switching System.
M.R. Lakshman and M.N. Rao, Purification and Characterization of Cellular Carotenoid-Binding Protein from Mammalian Liver.
J.P. Landrum, R.A. Bone, L.L. Moore, and C.M. Gomez, Analysis of Zeaxanthin Stereoisomer Distribution within Individual Human Retinas.
Subject Index.
H. Alho and J. Leinonen, Total Antioxidant Activity Measured by Chemiluminescence Methods.
I.F.F. Benzie and J.J. Strain, Ferric Reducing/Antioxidant Power Assay: Direct Measure of "Total Antioxidant Activity" of Biological Fluids, and a Modified Version (FRASC) Which Enables the Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration.
Y. Noda, M. Kohno, A. Mori, and L. Packer, Automated ESR Free Radical Detector Assays for Antioxidant Activity in Natural Extracts.
A. Kontush and U. Beisiegel, Measurement of Oxidizability of Blood Plasma.
G. Cao and R.L. Prior, Measurement of Oxygen Radical Absorbance Capacity in Biological Samples.
Vitamin C:
M. Levine, Y. Wang, and S.C. Rumsey, Analysis of Ascorbic and Dehydroascorbic Acid in Biological Samples.
A.M. Bode and R.C. Rose, Analysis of Water-Soluble Antioxidants by High-Performance Liquid Chromatography with Electrochemical Detection.
J. Lykkesfeldt and B.N. Ames, Ascorbic Acid Recycling in Rat Hepatocytes as a Measurement of Antioxidant Capacity: Decline with Age.
Polyphenols and Flavonoids:
L.C. Bourne and C. Rice-Evans, Method for Detecting and Measuring Bioavailability of Phenolics and Flavonoids in Humans: Pharmacokinetics of Urinary Excretion of Dietary Ferulic Acid.
T. Goto and Y. Yoshida, Simultaneous Analysis of Individual Catechins and Caffeine in Green Tea.
A.L. Waterhouse, S.F. Price, and J.D. McCord, Reverse Phase HPLC Methods for Analysis of Wine Polyphenols.
D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by High-Performance Liquid Chromatography.
D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by Gas Chromatography-Mass Spectrometry.
V.L. Singleton, R. Orthofer, and R.M. Lamuela-Raventós, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent.
V. Cheynier, J.-M. Souquet, E. LeRoux, S. Guyot, and J. Riguad, Size Separation of Condensed Tannins by Normal-Phase HPLC.
R.M. Lamuela-Raventós and A. Waterhouse, Resveratrol and Piceid in Wine.
E.N. Frankel, Antioxidant Activity by Headspace Gas Chromatography of Volatile Oxidation Products of w-6 and w-3 p Polyunsaturated Lipids.
P.C.H. Hollman, D.P. Venema, S. Khokhar, and I.C.W. Arts, Determination of Tea Catechins by Reversed Phase HPLC.
A.S. Pannala, S. Singh, and C. Rice-Evans, Flavonoids as Peroxynitrite Scavengers in Vitro.
Thiols:
C.K. Sen, S. Roy, S. Khanna, and L. Packer, Determination of Oxidized and Reduced Lipoic Acid Using High Performance Liquid Chromatography and Coulometric Detection.
C.K. Sen, S. Roy, and L. Packer, Flow Cytometric Determination of Cellular Thiols.
L.A. Ridnour, R.A. Winters, N. Ercal, and D.R. Spitz, Measurement of Glutathione, Glutathione Disulfide and Other Thiols in Mammalian Cell and Tissue Homogenates Using HPLC Separation of N-(1n-pyrenyl)maleimide Derivatives.
M. Asensi, J. Sastre, F.V. Pallardo, A. Lloret, M. Lehner, J. Garcia-De-La Asuncion, and J. Viña, GSH:GSSG Ratio as an Indicator of Oxidative Stress Status and DNA Damage.
J. Chaudiere, N. Aguini, and J.-C. Yadan, Nonenzymatic Colorimetric Assay of Glutathione in the Presence of Other Mercaptans.
H. Baker, B. DeAngelis, E.R. Baker, and S.H. Hutner, Protozoological Method for Assaying Lipoate in Human Biologic Fluids and Tissue.
O. Tirosh, Y. Katzhendler, Y. Barenholz, and R. Kohen, Antioxidant Activity of Amidothionophosphates.
K. Traber and L. Packer, Quantitation of Anethole Dithiolthione Using High Pressure Liquid Chromatography with Electrochemical Detection. Vitamin E and Coenzyme Q10:
D.C. Liebler, J.A. Burr, and A.J.L. Ham, Gas Chromatography-Mass Spectrometry Analysis of Vitamin E and Its Oxidation Products.
J.K.G. Kramer, R.C. Fouchard, and K.M.R. Kallury, Improved Method to Determine Vitamin E Forms in Tissues and Diets by High Performance Liquid Chromatography Using a Normal-Phase Diol Column.
M. Podda, C. Weber, M.G. Traber, R. Milbradt, and L. Packer, Sensitive HPLC Techniques for Simultaneous Determination of Tocopherols, Tocotrienols, Ubiquinols, and Ubiquinones in Biological Samples.
B. Finckh, A. Kontush, J. Commentz, C. Hübner, M. Burdelski, and A. Kohlschütter, HPLC Analysis of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma--Coulometric Electrochemical Detection.
C.-P. Aebischer, J. Schierle, and W. Schüep, Simultaneous Determination of Retinol, Tocopherols, Carotene Lycopene and Xanthophylls in Plasma by Means of Reversed Phase HPLC.
P.K. Witting, D. Mohr, and R. Stocker, Assessment of the Prooxidant Activity of Vitamin E in Human Low Density Lipoprotein and Plasma.
Carotenoids and Retinoids:
N. Pellegrini, R. Re, M. Yang, and C.A. Rice-Evans, Screening of Dietary Carotenoids and Carotenoid-Rich Fruit Extracts for Antioxidant Activities Applying the ABTs Radical Cation Decolorization Assay.
T. Wingerath, D. Kirsch, R. Kaufmann, W. Stahl, and H. Sies, Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry (MALDI-PSD-MS) in Carotenoid Analysis.
A. Mortensen and L.H. Skibsted, Carotenoid Photobleaching.
M.A. Livrea and L. Tesoriere, Interactions Between Vitamin A and Vitamin E in Liposomes and in Biological Contexts.
T.E. Gundersen and R. Blomhoff, On-Line Solid Phase Extraction and Isocratic Separation of Retinoic Acid Isomers in a Micro-bore Column Switching System.
M.R. Lakshman and M.N. Rao, Purification and Characterization of Cellular Carotenoid-Binding Protein from Mammalian Liver.
J.P. Landrum, R.A. Bone, L.L. Moore, and C.M. Gomez, Analysis of Zeaxanthin Stereoisomer Distribution within Individual Human Retinas.
Subject Index.
- Edition: 1
- Volume: 299
- Published: October 2, 1998
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780123917898
- Hardback ISBN: 9780121822002
- eBook ISBN: 9780080884103
JA
John N. Abelson
Affiliations and expertise
California Institute of Technology, Division of Biology, Pasadena, U.S.A.HS
Helmut Sies
Helmut Sies, MD, PhD (hon), studied medicine at the universities of Tübingen, Munich, and Paris. He was the professor and chair of the Institute for Biochemistry and Molecular Biology I at Heinrich-Heine-University Düsseldorf, Germany, where he is now professor emeritus. He is a member of the German National Academy of Sciences Leopoldina and was the president of the North Rhine-Westphalian Academy of Sciences and Arts. He was named ‘Redox Pioneer’; was the president of the Society for Free Radical Research International (SFRRI). Helmut Sies introduced the concept of “Oxidative Stress” in 1985, and was the first to reveal hydrogen peroxide as a normal constituent of aerobic cell metabolism. His research interests comprise redox biology, oxidants, antioxidants, micronutrients.
Affiliations and expertise
Heinrich-Heine-University Düsseldorf, GermanyMS
Melvin I. Simon
Affiliations and expertise
The Salk Institute, La Jolla, CA, USARead Oxidants and Antioxidants, Part A on ScienceDirect