Optical Techniques
Physical Techniques in Biological Research
- 2nd Edition - November 21, 2013
- Latest edition
- Editor: Gerald Oster
- Language: English
Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of… Read more
Physical Techniques in Biological Research, Second Edition, Volume I, Part A: Optical Techniques focuses on the methods and theory applied to relatively pure preparations of biological substances that are derived from cells or other tissue elements. This book covers a wide variety of nonoptical techniques. Organized into five chapters, this edition begins with an overview of the fundamental principles of operation of optical instruments that can be explained in terms of the simple operations of lenses or light rays. This text then examines the limits imposed on direct observation, either through an optical instrument or with the unaided eye, by the nature of light itself. Other chapters consider the potential of certain other microprobe methods. This book discusses as well the process of molecular spectroscopy. The final chapter deals with optical rotation and its dependence on wavelength. This book is a valuable resource for biologists, scientists, physicists, physical chemists, and research workers.
List of Contributors
Preface to the Second Edition of Volume I
Preface to the First Edition of Volume I
Contents of Volume IB
Chapter 1 The Light Microscope
I. Principles of Lenses and Lens Systems
II. Physical Theory
III. The Eye in Relation to Microscopy
IV. The Optical and Mechanical Systems of the Microscope
V. Adjustment and Use of the Microscope
VI. General Theory of Image Formation in the Microscope
References
Chapter 2 Electron Microscopy
I. The Limits of Optical Observation
II. Basic Principles of Electron Microscopy
III. Practical Differences between Optical and Electron Microscopy
IV. Elements of Electron Lenses
V. The Illuminating System
VI. The Imaging System
VII. Recording the Image
VIII. Requirements for High Performance
IX. Specimen Preparation
X. Artifacts
XI. The Scanning Electron Microscope
XII. The High Voltage Electron Microscope
XIII. Other Types of Electron Microscope
References
Chapter 3 The Microprobe Assay of Chemical Elements
I. Introduction
II. Components and Modes of Operation of the Standard Microprobe
III. Types of Instrumentation and Their Performance
IV. Spatial Resolution of the X-Ray Analysis
V. Sensitivity
VI. Quantitation
VII. Specimen Preparation
VIII. Damage to Specimens in the Electron-Probe Instrument
IX. Other Types of Microprobe Analysis
References
Appendix I. Manufacturers and Availability of Probe Instruments
Appendix II. Bibliography of Biological Applications of Electron-Probe X-Ray Microanalysis
Chapter 4 Infrared and Raman Spectroscopy
I. Introduction
II. Infrared Methods
III. Raman Methods
References
Chapter 5 Optical Rotatory Dispersion and Circular Dichroism
I. Introduction
II. Polynucleotides, RNA and DNA
III. Polypeptides and Proteins
IV. Polysaccharides
V. Concluding Remarks
References
Author Index
Subject Index
- Edition: 2
- Latest edition
- Published: November 21, 2013
- Language: English
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