Optical Techniques in Biological Research

List of Contributors

Series Introduction

Preface


1. Light Scattering

List of Symbols

I. Introduction

II. Elastic Light Scattering

III. Quasi-Elastic Light Scattering

IV. Laser Doppler Techniques

References


2. Raman Scattering

I. Introduction

II. Theoretical Background

III. Experimental Procedures

IV. Nonresonance Raman Scattering

V. Resonance Raman Scattering

VI. Intact Organelles

VII. New Techniques

VIII. Concluding Remarks

References


3. Fourier Transform Infrared Spectroscopy

I. Infrared Spectroscopy

II. Biological Applications

III. Techniques of Measurement

IV. Instrumental Considerations

V. Computational Problems in Fourier Transform Spectroscopy

VI. Summary and Conclusion

References

General References


4. Nanosecond Fluorescence Spectroscopy

I. Introduction

II. Measurement of Nanosecond Emission

III. Analysis of Nanosecond Fluorescence Data

IV. Analysis of Nanosecond Fluorescence Anisotropy

V. Applications

References


5. Circular Dichroism

I. Introduction

II. Methods

III. Application to Biological Problems

IV. Scattering Particles

V. Protein Molecules

VI. Extensions of Circular Dichroism Techniques

VII. Theory

VIII. Instrumentation

IX. Summary

References


6. Electron Microscopy

I. Introduction

II. Formation of Electron Images

III. The Instrument

IV. Specimen Preparation

V. Special Applications

VI. Image Processing

VII. Other Short-Wavelength Microscopes

VIII. Epilogue

Appendix I: Theory of Imaging

References

Index