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Odorant Binding and Chemosensory Proteins

  • 1st Edition, Volume 642 - August 20, 2020
  • Latest edition
  • Editors: Paolo Pelosi, Wolfgang Knoll
  • Language: English

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.… Read more

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Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Key features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of contents

    1. Genome mining and sequence analysis of chemosensory soluble proteins in arthropods
    2. Joel Vizueta, Paula Escuer, Alejandro Sánchez-Gracia and Julio Rozas

    3. Reconstructing protein coding sequences from ancient DNA
    4. Michael Hofreiter and Stefanie Hartmann

    5. Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs
    6. Jean-François Picimbon

    7. Proteomics of arthropod soluble olfactory proteins
    8. Francesca R. Dani and Giuseppe Pieraccini

    9. Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects
    10. Andrea Scaloni

    11. Bacterial expression and purification of vertebrate odorant-binding proteins
    12. Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loïc Briand

    13. Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography
    14. Anaïs Gaubert, Béatrice Amigues, Silvia Spinelli and Christian Cambillau

    15. Solution structure of insect CSP and OBPs by NMR
    16. Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone

    17. Stability of OBPs
    18. Nadja Hellmann

    19. Ligand-binding assays with OBPs and CSPs
    20. Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi

    21. Fine structure immunocytochemistry—An important tool for research on odorant-binding proteins
    22. Rudolf Alexander Steinbrecht

    23. Effect of OBPs on the response of olfactory receptors
    24. Bing Wang, Song Cao, Wei Liu and Guirong Wang

    25. Site-directed mutagenesis of odorant-binding proteins
    26. Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi

    27. Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing
    28. Yusuke Shiota and Takeshi Sakurai

    29. Electrochemical biosensing with odorant binding proteins
    30. Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu

    31. Electronic biosensors based on graphene FETs
    32. Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll

    33. Electronic biosensors based on EGOFETs
    34. Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli

    35. Gravimetric biosensors
    36. Khasim Cali, Elena Tuccori and Krishna C. Persaud

    37. Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins

Anil Bozdogan, Simone Hageneder and Jakub Dostalek

Product details

  • Edition: 1
  • Latest edition
  • Volume: 642
  • Published: August 20, 2020
  • Language: English

About the editors

PP

Paolo Pelosi

Paolo Pelosi is at AIT Austrian Institute of Technology GmbH, Austria
Affiliations and expertise
AIT Austrian Institute of Technology GmbH, Austria

WK

Wolfgang Knoll

Wolfgang Knoll is at AIT Austrian Institute of Technology GmbH, Austria
Affiliations and expertise
AIT Austrian Institute of Technology GmbH, Austria

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