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New Methods and Sensors for Membrane and Cell Volume Research

  • 1st Edition, Volume 88 - November 29, 2021
  • Latest edition
  • Editors: Irena Levitan, Michael Model
  • Language: English

New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-mem… Read more

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Description

New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest.

Key features

  • Describes novel FRET-based membrane sensors
  • Reviews selected non-optical approaches to membrane structure and dynamics
  • Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding

Readership

Wide range of researchers in academia and industry

Table of contents

On, in, and under membrane
M.M. Model and I. Levitan

1. Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell
Luca Mantovanelli, Bauke F. Gaastra and Bert Poolman

2. Current methods for studying intracellular liquid-liquid phase separation
Amber R Titus and Edgar E Kooijman

3. Investigating molecular crowding during cell division in budding yeast with FRET
Sarah Lecinski, Jack W. Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald and Mark C. Leake

4. The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC
Yulia Kolobkova, Sumaira Pervaiz and Tobias Stauber

5. Studying cell volume beyond cell volume
Michael A. Model

6. Membrane tension
Pei-Chuan Chao and Frederick Sachs

7. Methods for assessment of membrane protrusion dynamics
Jordan Fauser, Martin Brennan, Denis Tsygankov and Andrei V. Karginov

8. Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids
Irena Levitan

9. Fluorescence sensors for imaging membrane lipid domains and cholesterol
Francisco J. Barrantes

10. Mass spectrometry-based lipid analysis and imaging
Koralege C. Pathmasiri, Thu T.A. Nguyen, Nigina Khamidova and Stephanie M. Cologna

11. Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering
Ursula Perez-Salas, Yuri Gerelli, Lionel Porcar and Sumit Garg

Product details

  • Edition: 1
  • Latest edition
  • Volume: 88
  • Published: November 29, 2021
  • Language: English

About the editors

IL

Irena Levitan

Irena Levitan, PhD, is a Professor of Medicine and Bioengineering at the University of Illinois at Chicago. She received her PhD in Biophysics and Neurobiology at the Hebrew University of Jerusalem in 1994 and completed postdoctoral training at the Medical College of Pennsylvania and Institute for Medicine and Engineering at the University of Pennsylvania. Her research focuses on the biophysical properties of endothelial membranes and sub-membrane cytoskeleton. Specifically, the studies of her group, which combine computational and experimental biophysical approaches, provided the first comprehensive structural insights into cholesterol regulation of K+ channels. In 2012, she was named a Guyton Distinguished Lecturer “for her quantitative and biophysical work on cholesterol modulation of ion channels and how this can affect integrated organ function”. She and her group also discovered a paradoxical relationship between fluidity/deformability of the membrane and cell stiffness. In 2018, she was elected a Fellow of AIMBE for “outstanding contributions to our understanding of lipid-ion channel interactions, cellular biomechanics and vascular dysfunction under dyslipidemia”.
Affiliations and expertise
Professor of Medicine and Adjunct Professor of Pharmacology and Bioengineering, University of Illinois, Chicago, USA

MM

Michael Model

Michael Model got an M.S. in materials science from Russia and continued his education at the University of Michigan, where he received a Ph.D. in biophysics in 1995. Since 2004, he has been working at Kent State University, first as a microscopy facility manager and currently as an associate professor in the Department of Biological Sciences. While at Kent State, he developed a new microscopic method to measure cell volume, and that prompted him to turn to the physiology of cell volume regulation. He is also interested in the biological role and control of macromolecular crowding and continues to work on microscopic techniques and their applications.
Affiliations and expertise
Kent State University

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