Nanobiology of Tissue Repair and Regeneration
From Cell to System
- 1st Edition - June 1, 2027
- Latest edition
- Editor: Subhadip Ghatak
- Language: English
Nanobiology of Tissue Repair and Regeneration: From Cell to System offers the first comprehensive synthesis of how extracellular vesicles (EVs) and nanoscale biological system… Read more
Description
Description
Nanobiology of Tissue Repair and Regeneration: From Cell to System offers the first comprehensive synthesis of how extracellular vesicles (EVs) and nanoscale biological systems coordinate tissue repair from molecular signaling to systemic recovery. By integrating insights from nanotechnology, multi-omics, and regenerative biology, the book provides a unified framework that connects EV cargo biology to therapeutic outcomes—bridging a critical gap in current literature. This volume maps the entire regenerative continuum. Foundational chapters explore EV biogenesis, cargo profiling, and regenerative microenvironments, while advanced sections introduce emerging domains such as mitovesicles, programmable nanovesicles, and cross-kingdom RNA communication. Positioning EVs as central mediators of nanoscale communication, the text addresses urgent needs in precision therapy design for chronic wounds, neurodegenerative disorders, and fibrotic diseases. It integrates standardization guidelines (MISEV, EV-TRACK), scalable and compliant nanomedicine production strategies, and biomarker discovery frameworks to ensure both methodological rigor and clinical translation. Nanobiology of Tissue Repair and Regeneration: From Cell to System is structured as both a conceptual roadmap and a practical reference, the book equips regenerative medicine researchers, EV biologists, biomedical engineers, and clinician-scientists to navigate the complexities of reprogramming repair trajectories across diverse tissues and disease contexts. By uniting molecular mechanisms with system-level perspectives, it lays the groundwork for the next era of regenerative medicine—one that is molecularly informed, clinically scalable, and technologically integrated.
Key features
Key features
- Provides a systems-level understanding of how nanoscale communication governs tissue healing—from intracellular vesicle cargo to systemic repair outcomes
- Equips users with a unifying logic to connect disparate disciplines (EV science, tissue biology, bioengineering) and design more effective regenerative strategies
- Delivers targeted insights into EV roles in skin, liver, heart, kidney, brain, and musculoskeletal regeneration across diverse pathological settings
- Enhances reproducibility and standardization through MISEV-aligned methods (NTA, SEM, dSTORM, EV antibody arrays), empowering bench-level implementation
- Improves conceptual clarity through over 200 schematics, diagrams, and illustrative figures, aiding both teaching and applied research
Readership
Readership
Academic researchers, postdoctoral fellows, and graduate students in extracellular vesicle biology, regenerative medicine, nanomedicine, tissue engineering, and systems biology
Table of contents
Table of contents
Section I. Conceptual Foundations of Regenerative Nanobiology
1. Introduction to the Regenerative Continuum: From Cell State to System Identity
2. Principles of Tissue Repair, Plasticity, and Homeostatic Resilience
3. History and Future of Nanobiology in Regenerative Medicine
4. Extracellular Vesicles: Biogenesis, Cargo Logic, and Systems Function
5. Nanoscale Communication: Electrostatic, Glycan, and Biophysical Interfaces
Section II. Molecular Interfaces and Vesicular Cargo
6. Multi-Omics of Extracellular Vesicles
6.1 Transcriptomics: mRNA, miRNA, lncRNA
6.2 Proteomics and Phosphoproteomics
6.3 Lipidomics and Glycoproteomics
6.4 Single Vesicle Analysis and Cargo Stoichiometry
7. Actin Remodeling, Polarity, and Migration via EVs
8. Glycosylation and Surface Charge in Vesicle Targeting
9. Mitochondrial Transfer and Mitovesicles
10. Artificial Vesicles, Programmable Nanocarriers, and Synthetic Interfaces
11. Membrane-Bound Vesicles and Subtypes Beyond Exosomes
Section III. Cellular Origins and Intercellular Dialogues
12. Stem Cell Niches and Vesicle-Mediated Regulation
13. Macrophage–Keratinocyte Vesicle Exchange and Immune Modulation
14. Stromal–Epithelial Crosstalk in Matrix Remodeling
15. Endothelial and Pericyte-Derived EVs in Vascular Coordination
16. Mesenchymal Stromal Cell-Derived EVs and Immunomodulation
Section IV. Tissue and Organ-Specific Regeneration
17. Cutaneous Wound Healing and EV-Mediated Altruism
18. Myocardial Repair and Cardiac Fibroblast Vesicle Function
19. Liver Regeneration and Hepatic Zonal Vesicle Traffic
20. Brain-Derived EVs in Neuroinflammation and Neurorepair
21. Renal Tubular-Epithelial Crosstalk and Urinary Exosomes
22. Cartilage and Musculoskeletal Regeneration via EV Mechanotransduction
23. Lung, Gut, and Skin Barrier Tissues: Shared EV Regenerative Themes
24. Placental EVs: Maternal–Fetal Communication and Development
25. Adipose Tissue derived EV
26. EV in Transplantation
27. EV in Inflammation
Section V. Disease Contexts and Diagnostic Applications
28. Plasma and Wound Fluid Exosomes in Inflammation
29. CSF-Derived EVs in Neurodegeneration and CNS Disease
30. Cancer-Derived EVs in Tumor Biology and Therapy Resistance
31. Infection-Derived EVs and Host–Pathogen Vesicle Dynamics
32. EVs in Metabolic Dysfunction: From Adipose Tissue to Insulin Resistance
33. Exosomes in Aging and Senescence: Biomarkers and Functional Decline
34. EV in Sepsis
Section VI. Tools, Standards, and Translation Readiness
35. MISEV, EV-TRACK, and the Need for Standardization
36. Data Infrastructure: CDMS, IMMS, and FAIR Compliance
37. Imaging Vesicles In Vivo: Optical, Super-Resolution, and Quantitative Tools
38. Isolation, Purity, and Quantification Challenges in EV Research
39. Regulatory Frameworks and Clinical Trial Design for EV-Based Therapies
40. GMP-Compliant Manufacturing of Therapeutic EVs
41. Scalable Manufacturing and MISEV-Compliant Quality Control
42. Challenges and Opportunities in Clinical Trials of EV-Based Therapies
Section VII. Emerging Frontiers in Evolution and Engineering
43. Cross-Kingdom RNA Trafficking via Vesicles
44. Exosome-Induced Tunneling Nanotubes and Filopodia Formation
45. Plasma Membrane Repair as a Vesicle-Mediated Mechanism
46. AI and Systems Biology for Predicting Regenerative Outcomes
47. The Regenerative Atlas: Mapping Vesicular Influence Across Systems
Section VIII. Therapeutic Engineering and Precision Regeneration
48. Bioengineered EVs and Designer Vesicles
49. Nanomaterial–EV Hybrids for Targeted Therapy
50. Liquid Biopsy Platforms and Companion Diagnostics
51. Integration of Omics with Personalized Regenerative Interventions
Section IX. Future Directions
52. Artificial Cells and Programmable Nanovesicles
53. Autonomous Vesicle Networks and Synthetic Biology in Tissue Engineering
54. Human–Machine Integration: EVs in Bioelectronic Interfaces
Appendices
A. Glossary of Key Terms in EV Biology and Nanomedicine
B. Protocol Checklists and Reproducibility Standards o Nanoparticle Tracking Analysis (NTA) o Scanning Electron Microscopy (SEM) o Exosome Antibody Array Profiling o Super-Resolution Microscopy Techniques o dSTORM (Direct Stochastic Optical Reconstruction Microscopy) o MISEV-Compliant Isolation, Purification, and Quantification Protocols o Functional Assays and Vesicle Labeling Techniques
C. Public Databases and EV Resources (Vesiclepedia, ExoCarta, EV-TRACK)
D. Author Biographies and Institutional Affiliations:
E. Global EV Societies
1. Introduction to the Regenerative Continuum: From Cell State to System Identity
2. Principles of Tissue Repair, Plasticity, and Homeostatic Resilience
3. History and Future of Nanobiology in Regenerative Medicine
4. Extracellular Vesicles: Biogenesis, Cargo Logic, and Systems Function
5. Nanoscale Communication: Electrostatic, Glycan, and Biophysical Interfaces
Section II. Molecular Interfaces and Vesicular Cargo
6. Multi-Omics of Extracellular Vesicles
6.1 Transcriptomics: mRNA, miRNA, lncRNA
6.2 Proteomics and Phosphoproteomics
6.3 Lipidomics and Glycoproteomics
6.4 Single Vesicle Analysis and Cargo Stoichiometry
7. Actin Remodeling, Polarity, and Migration via EVs
8. Glycosylation and Surface Charge in Vesicle Targeting
9. Mitochondrial Transfer and Mitovesicles
10. Artificial Vesicles, Programmable Nanocarriers, and Synthetic Interfaces
11. Membrane-Bound Vesicles and Subtypes Beyond Exosomes
Section III. Cellular Origins and Intercellular Dialogues
12. Stem Cell Niches and Vesicle-Mediated Regulation
13. Macrophage–Keratinocyte Vesicle Exchange and Immune Modulation
14. Stromal–Epithelial Crosstalk in Matrix Remodeling
15. Endothelial and Pericyte-Derived EVs in Vascular Coordination
16. Mesenchymal Stromal Cell-Derived EVs and Immunomodulation
Section IV. Tissue and Organ-Specific Regeneration
17. Cutaneous Wound Healing and EV-Mediated Altruism
18. Myocardial Repair and Cardiac Fibroblast Vesicle Function
19. Liver Regeneration and Hepatic Zonal Vesicle Traffic
20. Brain-Derived EVs in Neuroinflammation and Neurorepair
21. Renal Tubular-Epithelial Crosstalk and Urinary Exosomes
22. Cartilage and Musculoskeletal Regeneration via EV Mechanotransduction
23. Lung, Gut, and Skin Barrier Tissues: Shared EV Regenerative Themes
24. Placental EVs: Maternal–Fetal Communication and Development
25. Adipose Tissue derived EV
26. EV in Transplantation
27. EV in Inflammation
Section V. Disease Contexts and Diagnostic Applications
28. Plasma and Wound Fluid Exosomes in Inflammation
29. CSF-Derived EVs in Neurodegeneration and CNS Disease
30. Cancer-Derived EVs in Tumor Biology and Therapy Resistance
31. Infection-Derived EVs and Host–Pathogen Vesicle Dynamics
32. EVs in Metabolic Dysfunction: From Adipose Tissue to Insulin Resistance
33. Exosomes in Aging and Senescence: Biomarkers and Functional Decline
34. EV in Sepsis
Section VI. Tools, Standards, and Translation Readiness
35. MISEV, EV-TRACK, and the Need for Standardization
36. Data Infrastructure: CDMS, IMMS, and FAIR Compliance
37. Imaging Vesicles In Vivo: Optical, Super-Resolution, and Quantitative Tools
38. Isolation, Purity, and Quantification Challenges in EV Research
39. Regulatory Frameworks and Clinical Trial Design for EV-Based Therapies
40. GMP-Compliant Manufacturing of Therapeutic EVs
41. Scalable Manufacturing and MISEV-Compliant Quality Control
42. Challenges and Opportunities in Clinical Trials of EV-Based Therapies
Section VII. Emerging Frontiers in Evolution and Engineering
43. Cross-Kingdom RNA Trafficking via Vesicles
44. Exosome-Induced Tunneling Nanotubes and Filopodia Formation
45. Plasma Membrane Repair as a Vesicle-Mediated Mechanism
46. AI and Systems Biology for Predicting Regenerative Outcomes
47. The Regenerative Atlas: Mapping Vesicular Influence Across Systems
Section VIII. Therapeutic Engineering and Precision Regeneration
48. Bioengineered EVs and Designer Vesicles
49. Nanomaterial–EV Hybrids for Targeted Therapy
50. Liquid Biopsy Platforms and Companion Diagnostics
51. Integration of Omics with Personalized Regenerative Interventions
Section IX. Future Directions
52. Artificial Cells and Programmable Nanovesicles
53. Autonomous Vesicle Networks and Synthetic Biology in Tissue Engineering
54. Human–Machine Integration: EVs in Bioelectronic Interfaces
Appendices
A. Glossary of Key Terms in EV Biology and Nanomedicine
B. Protocol Checklists and Reproducibility Standards o Nanoparticle Tracking Analysis (NTA) o Scanning Electron Microscopy (SEM) o Exosome Antibody Array Profiling o Super-Resolution Microscopy Techniques o dSTORM (Direct Stochastic Optical Reconstruction Microscopy) o MISEV-Compliant Isolation, Purification, and Quantification Protocols o Functional Assays and Vesicle Labeling Techniques
C. Public Databases and EV Resources (Vesiclepedia, ExoCarta, EV-TRACK)
D. Author Biographies and Institutional Affiliations:
E. Global EV Societies
Product details
Product details
- Edition: 1
- Latest edition
- Published: June 1, 2027
- Language: English
About the editor
About the editor
Subhadip Ghatak
Editor
Dr. Subhadip Ghatak is an Associate Professor of Surgery at the University of Pittsburgh School of Medicine and a core faculty member at the McGowan Institute for Regenerative Medicine. With over 15 years of research experience at the intersection of extracellular vesicle biology, nanomedicine, and tissue repair, Dr. Ghatak has led numerous high-impact studies on miRNA regulation, tissue reprogramming, and exosome-mediated intercellular communication in wound healing. His pioneering contributions include the development of targeted lipid nanoparticle systems and tissue nanotransfection (TNT) for in vivo gene delivery, as well as the characterization of dysfunctional exosomes in diabetic wounds. He is also an active contributor to EV standardization efforts, including the MISEV guidelines and the EV-TRACK task force.
Dr. Ghatak’s scientific output includes over 50 peer-reviewed publications, multiple patents, and significant roles in NIH- and state-funded research projects focused on wound healing, and regenerative biology.
Affiliations and expertise
Associate Professor of Surgery, University of Pittsburgh School of Medicine, Core Faculty, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA