Molecular Mechanisms in the Control of Gene Expression
- 1st Edition - January 1, 1976
- Imprint: Academic Press
- Editors: Donald P. Nierlich, W.J. Rutter, C. Fred Fox
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 4 4 1 2 - 9
- Hardback ISBN:9 7 8 - 0 - 1 2 - 5 1 8 5 5 0 - 9
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 7 3 9 3 - 8
Molecular Mechanisms in the Control of Gene Expression documents the proceedings of the ICN-UCLA conference on Molecular Mechanisms in the Control of Gene Expression, organized… Read more
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Request a sales quoteMolecular Mechanisms in the Control of Gene Expression documents the proceedings of the ICN-UCLA conference on Molecular Mechanisms in the Control of Gene Expression, organized through the Molecular Biology Institute of UCLA, held in Keystone, Colorado, 21-26 March 1976. The conference focused on three topics: the action of repressors on specific nucleotide sequences in DNA; how DNA and histones are intertwined in eucaryotic chromosomes; and in the development of new techniques that appear to lift genes from complex genomes. The volume contains 65 chapters organized into nine parts. The papers in Part I examine the organization of prokaryotic and eukaryotic chromosomes. Part II presents studies on the interaction of RNA a polymerase and regulatory molecules with defined DNA sites. Parts III and IV focus on RNA polymerases of eukaryotes and the regulation of transcription in eukaryotic systems, respectively. Part V contains papers dealing with nucleic acid sequences, transcription, and processing. Part VI covers cellular aspects in the study of gene expression. Part VII takes up cloning while Part VIII is devoted to genetic analysis through restriction mapping and molecular cloning. Finally, Part IX summarizes the recent progress reported at the conference and also indicates some of the limitations that can be placed upon interpretation of data.
Preface
I. Organization of Prokaryotic and Eukaryotic Chromosomes
1. The Structure of Chromatin and Its Reconstruction
2. X-Ray Diffraction From Isolated Repeat Units of Chromatin
3. Nucleoprotein Core Particles in Chromatin Subunits: Existence of a Complex of Eight Histones and 140 Nucleotide Pairs of DNA
4. Histone 2A-2B-4 Interactions within Chromatin
5. The Site of Trimethylpsoralen Cross-Linking in Chromatin
6. The Isolation, Characterization and Suggested Structure of Euchromatic Segments from Mouse TLT Hepatoma Chromatin
7. Chromatin Assembly. Sites of Association of Newly Synthesized Chromatin Proteins on DNA
8. Autoradiographic Visualization and Sedimentation Properties of Unfolded Bacterial Nucleoid DNA
9. Thermoplasma Acidophilum: Studies on a Prokaryote That Contains a Histone-like Protein
10. Factors Which Control DNA Packaging May Influence the Control of Transcription
II. Interaction of RNA Polymerase and Regulatory Molecules with Defined DNA Sites
11. Transcriptional and Translational (?) Control of the λ Repressor Gene (cl)
12. Regulation of Transcription Initiation and Termination in the Control of Expression of the Tryptophan Operon of E. coli
13. Positive Control of the Temporal Program of Bacteriophage SP01 Gene Expression by Phage and Host Specified Subunits of RNA Polymerase
14. DNA-Binding Specificity of a Positively Regulating RNA Polymerase
15. Characterization of the Promoter of the T4 tRNA Operon
16. Chemical Modification of Supercoiled DNA: Effect on the Rate of Transcription
17. The Interaction of Chemically Synthesized 21 Base Pair Lac Operator with the Lac Repressor
18. Synthesis of Lac and λ Operator DNA Sequences
19. Reconstitution of Operator DNA-Active Lactose Repressor from Subunits
20. Electron Microscopy of Glutamine Synthetase-DNA Interactions
21. Regulation of Transcription by Peptide Antibiotics
III. RNA Polymerases of Eukaryotes: Transcription and Specificity
22. Characteristics of an In Vitro System Which Transcribes Viral RNA from Chromatin
23. Selective Transcription of the 5S RNA Genes in Isolated Chromatin by RNA Polymerase III
24. Oocyte RNA Polymerase of Xenopus Laevis: In Vitro Transcription of Amplified Ribosomal DNA
25. Localization of RNA Polymerase on Drosophila Polytene Chromosomes by Indirect Immunofluorescence
26. Subclasses of RNA Polymerase in the Urchin Embryo
27. Multiple RNA Polymerases from Acanthamoeba castellanii: Lack of Alteration in Subunit Architecture and Levels during Encystment
28. DNA-Dependent RNA Polymerases from Higher Plants
29. Studies on the Inhibition of Transcription by the Hepatocarcinogen N-Hydroxy-2-Acetylaminofluorene
IV. Regulation of Transcription in Eukaryotic Systems
30. The Relationship between Chromatin Structure and Transcription
31. The Synthesis, Isolation, Amplification, and Transcription of the Ovalbumin Gene
32. Kinetics of Ovalbumin and Conalbumin mRNA Induction by Estrogen and Progesterone
33. DNA-Dependent RNA Polymerase I and II Activation by 17ß-Estradiol-Receptor Complex on Liver Chromatin from Immature Chicks
34. Effect of Estrogen on Gene Expression: Vitellogenin Synthesis May Be Regulated at the Level of Both Transcription and Translation
35. Selective Transcription of the Euglena gracilis Chloroplast Chromosome In Vitro
36. Products of Cell-Free RNA Synthesis Using Conditions Preventing Initiation and Processing
V. Nucleic Acid Sequences, Transcription and Processing
37. Sequences of SV40 DNA
38. Complementary Sequences in Heterogeneous Nuclear RNA
39. Purification of Globin mRNA and Detection of Its Presumptive Precursor
40. The Complexity of Nuclear RNA and Messenger RNA m the Uninduced Friend Cell
41. A Comparison of the Sizes of Messenger RNAs Coding for Lysozyme (Gene 3.5) in T7-Infected RNase III+ and RNase III- Strains of Escherichia coli
42. Processing of Ribosomal RNA in E. coli
43. RNA Unwinding Proteins: Mechanism of Acrion and Apparent Cellular Location
44. Polynucleoüde Binding Properties of E. coli Ribosomal Protein SI
45. Kinetic Studies on the Hybridization of RNA to Double Stranded DNA
VI. Cellular Aspects in the Study of Gene Expression
46. Nuclear and Cytoplasmic Roles in Determination of Cellular Phenotype
47. Nuclear Transplantation with Mammalian Cells
48. Red Cell Microinjection of Transfer RNA Molecules
VU. Cloning and Cloning Vehicles
49. Construction and Characterization of Cloning Vehicles
50. A Mutation Amplifying the Genes Carried by the pi-Histidine Plasmid
51. The Fidelity of Replication of Mouse Mitochondrial DNA-pSC101 Recombinant Plasmids Grown in E. coli K12
52. Genetic Selections and the Cloning of Prokaryotic and Eukaryotic Genes
53. The Production of Proteins by Bacterial Plasmids Containing Eukaryotic DNA Fragments
54. In Vitro Synthesis and Molecular Cloning of Eukaryotic Structural Genes
55. Insertion of Rabbit Globin Sequences into E. coli Plasmids
VIII. Genetic Analysis through Restriction Mapping and Molecular Cloning
56. Cloning of Drosophila melanogaster ECORI DNA Fragment
57. Initial Studies of Sea Urchin DNA Sequence Organization by Molecular Cloning
58. Use of Molecular Cloning in the Genetic and Functional Analyses of the Plasmid F
59. Cloning of the Ribosomal RNA Genes of Yeast
60. Physical Mapping of SV40-Lambda Hybrid Genomes
61. Interspersion of Inverted and Middle Repeated Sequences within the Genome of the Silkworm, Bombyx mori
62. Organization of the Ribosomal Genes of Dictyostelium
63. Physical Mapping of Bacteriophage DNA by Exonuclease III and Endodeoxyribonuclease BAMH1
64. Isolation and Transcription of T4 DNA Fragments Containing the T4 tRNA Genes
IX. Summing up
65. Perspective and Trends in Gene Expression
Author Index
Subject Index
- Edition: 1
- Published: January 1, 1976
- No. of pages (eBook): 668
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9781483244129
- Hardback ISBN: 9780125185509
- eBook ISBN: 9781483273938
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