Molecular Genetics Pt 3

Contributors to Part III

Preface

Contents of Part I and Part II

Chapter I The Role of Restriction Endonucleases in Molecular Genetics

I. Introduction

II. General Properties of Restriction Enzymes

III. Basic Aspects of Restriction Enzyme Technology

IV. Applications of Restriction Enzyme Technology to the Study of Chromosome Structure and Function

V. Recombinant DNA Technology

VI. Concluding Remarks

References

Chapter II DNA Topoisomerases: Enzymes That Catalyze the Concerted Breaking and Rejoining of DNA Backbone Bonds

I. Introduction

II. Eco DNA Topoisomerase I

III. Other Prokaryotic DNA Topoisomerases

IV. Eukaryotic DNA Topoisomerases

V. Possible Biological Roles of DNA Topoisomerases

VI. Other Proteins with Properties Similar to DNA Topoisomerases

References

Chapter III Enzymatic Methylation of DNA: Patterns and Possible Regulatory Roles

I. Introduction

II. Restriction and Modification in Bacterial Cells

III. Sites of Methylation in DNA

IV. Patterns of Methylation of DNA and the Cell Division Cycle

V. Changes in Methylation during Differentiation and Development

VI. Methylating Enzymes Isolated from Cells

VII. Inactivation or Activation of the X Chromosome

VIII. Biological Clocks in Cell Division

IX. Hypothesis on the Control of Replication by Methylation

X. Other Models for Regulatory or Developmental Roles of Methylation

Addendum

References

Chapter IV Transcriptional Units in Eukaryotic Chromosomes

I. Introduction

II. Genome Organization and Transcription

III. Multiple-Copy Genes

IV. Single-Copy Genes

V. Perspectives

References

Chapter V Recognition and Control Sequences in Nucleic Acids

I. Introduction

II. Transcription

III. Regulation of Transcription by Sequence-Specific DNA Binding Proteins

IV. DNA Synthesis

V. Codon Selection in mRNA

VI. A Brief Overview of Other Sequences That Play Roles in Gene Expression

VII. Rapid Nucleic Acid Sequencing Methods

References

Chapter VI Nucleosomes: Composition and Substructure

I. Introduction

II. Histones

III. Nucleosomes—Discovery, Isolation, and Composition

IV. Nucleosome Structure

V. Concluding Remarks

References

Chapter VII Nucleosomes and Higher Levels of Chromosomal Organization

I. Introduction

II. The Concept of the Chromosome Fiber

III. Components of Chromosome Fibers

IV. Molecular Organization of Chromosome Fibers

V. Organization of Fibers into Chromosomes

VI. Functional Aspects of Chromosome Fiber Organization

References

Index