Modern Methods in the Analysis and Structural Elucidation of Mycotoxins
- 1st Edition - December 2, 2012
- Editor: Richard J. Cole
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 1 4 5 3 4 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 4 8 5 3 - 5
Modern Methods in the Analysis and Structural Elucidation of Mycotoxins presents available methods of analysis and structural elucidation of mycotoxins by recognized experts in the… Read more

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Request a sales quoteModern Methods in the Analysis and Structural Elucidation of Mycotoxins presents available methods of analysis and structural elucidation of mycotoxins by recognized experts in the various disciplines. The approach in each chapter of the book is to present each method initially in theoretical terms and then to review the method as it specifically applies to the analysis and/or structural elucidation of mycotoxins. Comprised of 15 chapters, the book's opening chapters deal with screening, sampling, and survey methods for mycotoxins and toxigenic fungi. This is followed by chapters dealing mostly with methods for structural elucidation, such as NMR and X-ray crystallography and IR and UV spectroscopy, as well as biosynthetic techniques. Significant chapters consider the analytical methods for mycotoxin analyses, including enzyme-linked immunosorbent assay system and tandem mass spectrometry. The concluding chapter examines the mycotoxin analytical problem in taxonomic or ecological terms. This book is of value to food and feed researchers, scientists, and manufacturers who are interested in product contamination control.
Preface
1 Biological Screening Methods for Mycotoxins and Toxigenic Fungi
I. Introduction
II. Animal Assays
III. Plant Assays
IV. Microbiological Assays
V. Conclusion
References
2 Sampling and Sample Preparation Methods for Mycotoxin Analysis
I. Introduction
II. Distribution of Mycotoxins within the Product
III. Sources of Error in Mycotoxin Tests
IV. Error Reduction
V. Sampling Procedures
VI. Subsampling Procedures
VII. Preservation of Samples
VIII. Survey Studies
IX. Quality Control
X. Experimentation
References
3 Chemical Survey Methods for Mycotoxins
I. Introduction
II. Aflatoxins
III. Zearalenone
IV. Trichothecenes
V. Ochratoxins
VI. Sterigmatocystin
VII. Conclusion and Future Developments
References
4 Application of Ultraviolet and Infrared Spectroscopy in the Analysis and Structural Elucidation of Mycotoxins
I. Introduction
II. Infrared Spectroscopy
III. Ultraviolet Spectroscopy
IV. Conclusion
References
5 Application of Nuclear Magnetic Resonance to Structural Elucidation of Mycotoxins
I. Introduction
II. Strategies in Structural Determination
III. One-Dimensional Methods
IV. Spectral Editing in 13C NMR Spectra
V. Two-Dimensional Methods
VI. Carbon-Carbon Connectivity
VII. Biosynthetic Studies
References
6 X-Ray Crystallography as a Tool for Identifying New Mycotoxins
I. Introduction
II. History and Description of the Single-Crystal X-Ray Diffraction Experiment
III. Advantages and Limitations of Using X-Ray Diffraction
IV. X-Ray Diffraction of Mycotoxins
V. Conclusions
References
7 Application of Biosynthetic Techniques in the Structural Studies of Mycotoxins
I. Introduction
II. Techniques
III. Biosynthesis of Polyketides
IV. Amino Acids in the Biosynthesis of Mycotoxins
References
8 Immunoassays for Mycotoxins
I. Introduction
II. Production of Antibody against Mycotoxins
III. Characterization of Antibody
IV. Radioimmunoassay for Mycotoxins
V. Enzyme-Linked Immunosorbent Assay for Mycotoxins
VI. Immunohistochemical Methods and Other Studies
VII. Concluding Remarks
References
9 Thin-Layer Chromatography/High-Performance Thin-Layer Chromatography as a Tool for Mycotoxin Determination
I. Introduction
II. Thin-Layer Chromatographic Procedures for Mycotoxins
III. Analysis of Individual Mycotoxins
IV. Conclusions
References
10 Gas Chromatography in Mycotoxin Analysis
I. Introduction
II. Practical Aspects of Gas Chromatography
III. Use of Gas Chromatography in the Analysis of Mycotoxins
References
11 High-Performance Liquid Chromatography and Its Application to the Analysis of Mycotoxins
I. Introduction
II. High-Performance Liquid Chromatographic Instrumentation and Methodology
III. Manipulation of High-Performance Liquid Chromatographic Conditions
IV. High-Performance Liquid Chromatography: A Literature Review
V. Conclusions
References
12 Gas Chromatographic-Mass Spectrometric Analysis of Mycotoxins
I. Introduction
II. History and Instrumentation
III. Comparison of Mass Spectrometry Techniques
IV. Quantitative Analysis
V. Mycotoxins
VI. Conclusions
References
13 Mass Spectra of Selected Trichothecenes
I. Introduction
II. Application to Selected Trichothecene Mycotoxins
III. Analysis of a Mixture of Trichothecenes by Gas Chromatography and Identification of Some of the Components by Searching a Computer Library of Positive Chemical Ionization Spectra
IV. Conclusions
References
14 Mass Spectrometry-Mass Spectrometry as a Tool for Mycotoxin Analysis
I. Introduction
II. Mass Spectrometry-Mass Spectrometry Instrumentation
III. Mass Spectrometry-Mass Spectrometry Techniques and Experiments
IV. Examples of Mass Spectrometry-Mass Spectrometry of Mycotoxins
References
15 Taxonomic Approaches to Mycotoxin Identification (Taxonomic Indication of Mycotoxin Content in Foods)
I. Introduction
II. Factors Affecting Mycotoxin Production
III. Mycological Examination of Foods
IV. Mycotoxins and Fungal Taxonomy
V. Important Genera Containing Toxigenic Isolates
References
Index
- No. of pages: 486
- Language: English
- Edition: 1
- Published: December 2, 2012
- Imprint: Academic Press
- Paperback ISBN: 9780124145344
- eBook ISBN: 9780323148535
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