Mitosis and Meiosis Part A

Preface
Helder Maiato and Melina Schuh

1. Assays for the spindle assembly checkpoint in cell culture
Chiara Marcozzi and Jonathon Pines

2. Quantitative methods to measure aneuploidy and chromosomal instability
Kristina M. Godek and Duane A. Compton

3. Dissecting the role of the tubulin code in mitosis
Luísa T. Ferreira, Ana C. Figueiredo, Bernardo Orr, Danilo Lopes and Helder Maiato

4. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis
Kara L. McKinley

5. Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells
Bramwell G. Lambrus, Tyler C. Moyer and Andrew J. Holland

6. Small molecule tools in mitosis research
Franziska Teusel, Lars Henschke and Thomas U. Mayer

7. Optogenetic control of mitosis with photocaged chemical dimerizers
Huaiying Zhang, David M. Chenoweth and Michael A. Lampson

8. Measuring mitotic forces
Anna A. Ye and Thomas J. Maresca

9. Employing the one-cell C. elegans embryo to study cell division processes
Neil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur, Sebastian Gomez, Taekyung Kim, Bram Prevo, Renat Khaliullin, Kian-Yong Lee, Midori Ota, Rebecca Green, Karen Oegema and Arshad Desai

10. A cell-free system of Drosophila egg explants supporting native mitotic cycles
Jorge de-Carvalho, Ojas Deshpande, Catarina Nabais and Ivo A. Telley

11. Living Xenopus oocytes, eggs, and embryos as models for cell division
Ani Varjabedian, Angela Kita and William Bement

12. Spreading of chromosomes from mouse oocytes and mammalian cultured cells for light microscopic analysis
Mariana C.C. Silva, Gordana Wutz, Kikuë Tachibana and Jan-Michael Peters

13. In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachments
Manas Chakraborty, Ekaterina Tarasovetc and Ekaterina L. Grishchuk

14. Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry
Itaru Samejima and William C. Earnshaw

15. Purification of kinetochores from the budding yeast Saccharomyces cerevisiae
Amitabha Gupta, Rena K. Evans, Lori B. Koch, Aimee J. Littleton and Sue Biggins

16. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae
Rokas Grigaitis, Aitor Susperregui, Philipp Wild and Joao Matos

17. Single-nucleus Hi-C of mammalian oocytes and zygotes
Johanna Gassler, Ilya M. Flyamer and Kikuë Tachibana

18. Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells
Hidetaka Miyauchi, Hiroshi Ohta and Mitinori Saitou

19. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos
Greg FitzHarris, John Carroll and Karl Swann

20. Single cell genomics to study DNA and chromosome changes in human gametes and embryos
Robert C. Blanshard, Chongyi Chen, X. Sunney Xie and Eva R. Hoffmann

21. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes
Hirohisa Kyogoku, Shuhei Yoshida and Tomoya S. Kitajima