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Elsevier

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

  • 1st Edition, Volume 147 - August 27, 2018
  • Latest edition
  • Editors: Matthieu Piel, Junsang Doh, Daniel Fletcher
  • Language: English

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial w… Read more

Description

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration.

Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more.

Key features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on both mitosis and meiosis
  • Includes relevant, analysis based topics

Readership

Researchers and academics in the cell biology field

Table of contents

Section 1 - Microfluidics for micro-organisms

1. Temperature control and drug delivery for cell division cycle control in fission yeast
Damien Coudreuse

2. H2O2 stress response in budding yeast
Gilles Charvin

3. Antibiotic resistance in bacteria
Bob Austin and Julia Bos

4. Metabolism in bacteria
Andrew Griffiths and Philippe Nghe

5. Fluidized beds for bacterial sorting and amplification
Stephanie Descroix and Jean-Louis Viovy

Section 2 - Microfluidics for cell culture and cell sorting of mammalian cells

6. Hydrogel microwells
Samy Gobaa

7. Cell sorting with conical filters
Yong Chen and Olivier Shi

8. Directed neuronal cell culture
Catherine Villard and Jean-Louis Viovy

Section 3 - Microfluidics for cell migration

9. Immune cells migration in complexe environments
Michael Sixt

10. Neutrophiles migration in health and disease
Daniel Irimia

11. Cell guidance by physical cues
Daniel Riveline

12. Stable gradients in gels of extracellular matrix for cancer cell migration
Danijela Vignjevic

Section 4 - Microfluidics for cell mechanics

13. Size based cell sorting and single cell mechanobiology
Chwee Teck Lim

14. High throughput measure of single cell deformability/stiffness
Jochen Guck

15. Host-microbe interactions
John McKinney

16. Cell confinement and intracellular crowding
Liam Holt, Morgan Delarue and Oskar Hallatschek

Section 5 - Microfluidics for Single cell analytics

17. Quantification of single cell secretion
Andrew Griffiths

18. Mechanical cell disruption
Wenhui Wang

Product details

  • Edition: 1
  • Latest edition
  • Volume: 147
  • Published: August 29, 2018
  • Language: English

About the editors

MP

Matthieu Piel

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.
Affiliations and expertise
Systems Biology of Cell Division and Cell Polarity, Cell Biology and Cancer Department, Institut Curie, Paris, France Institut Pierre Gilles de Gennes for Microfluidics, Paris, France

JD

Junsang Doh

Junsang Doh is an associate professor of Mechanical Engineering/Interdisciplinary Bioscience and Bioengineering (I-Bio) in POSTECH, South Korea. Prof. Doh’s group develops and utilizes engineering tools such as microfabrication/imaging/mechanics to study fundamental aspects of immune cell behaviors, including synapse-based cell-cell interactions and motility under complex microenvironments, in the context of cancer immunotherapy.
Affiliations and expertise
Associate Professor, Department of Mechanical Engineering, School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology

DF

Daniel Fletcher

Dr. Fletcher and his team develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. His lab specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of his work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases.
Affiliations and expertise
Purnendu Chatterjee Chair in Engineering Biological Systems, Department of Bioengineering, University of California Berkeley; Scientist and Deputy Division Director, Physical Biosciences Division, Lawrence Berkeley National Laboratory

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