Microfluidic Cell Culture Systems

Preface

List of Contributors

Part 1: Materials and Fabrication Methods

Chapter 1. Microfluidic Cell Culture Platforms with Embedded Nanoscale Features

1.1 Introduction

1.2 Engineering of nanoscale features

1.3 Assembly of PDMS-based microfluidic platforms

1.4 Microfluidic platforms with embedded nanoscale features for cell studies

1.5 Summary

Acknowledgment

References

Chapter 2. Microvascular Networks for Tissue Engineering

2.1 Introduction

2.2 Characteristics of branched vascular networks

2.3 Fabrication of 2-D microvascular networks

2.4 Fabrication of 3-D microvascular networks

2.5 Microchannel topologies

2.6 Engineering meets biology: toward tissue engineering applications

2.7 Outlook and future challenges

Acknowledgments

References

Chapter 3. Microfluidics for Engineering 3D Tissues and Cellular Microenvironments

3.1 Introduction

3.2 Fabricating 3D tissue scaffolds using microfluidics

3.3 Dynamic 3D cell cultures within PDMS microfluidic devices

3.4 Hydrogel-based microfluidic culture devices and tissue scaffolds

3.5 Conclusion and future directions

References

Chapter 4. Fabrication of Advanced Microcontainer Arrays for Perfused 3D Cell Culture in Microfluidic Bioreactors

4.1 Introduction

4.2 Micromolding of cell container arrays

4.3 Introducing porosity

4.4 Functionalization of cell container arrays

4.5 Integration into microfluidic bioreactors

4.6 Conclusion

References

Chapter 5. Mechanobiological Approaches for the Control of Cell Motility

5.1 Introduction

5.2 Passive control of cell motility

5.3 Active control of cell motility

5.4 Summary

References

Chapter 6. Transport Models for Three-Dimensional Cell Culture Systems

6.1 Introduction

6.2 Fluid flow in cell culture systems

6.3 The theory of mass transport

6.4 Binding kinetics

6.5 Nondimensionalization

6.6 Order of magnitude analysis

6.7 Bulk parameter models

6.8 Examples

6.9 Microfluidic approaches for flow and transport control

6.10 Conclusion

Part 2: Tissue Engineering Strategies

Chapter 7. Microfluidic Systems for Controlling Stem Cells Microenvironments

7.1 Introduction

7.2 Microfluidic elements for cell culture

7.3 Controlling cellular microenvironments

7.4 Challenges and outlook

Acknowledgments

References

Chapter 8. Vascularization of Microfluidic Hydrogels

8.1 Introduction

8.2 Design criteria for microfluidic scaffolds

8.3 Forming and vascularizing microfluidic gels

8.4 Design considerations

8.5 Design algorithm

8.6 Summary

Acknowledgments

References

Chapter 9. Microfluidic Vascular Networks for Engineered Tissues

9.1 Introduction

9.2 3D Microfluidics fabrication techniques

9.3 Materials for microfluidic vasculature

9.4 Conclusion

References

Chapter 10. Microfluidic Approaches Toward Pulmonary Tissue Constructs

10.1 Introduction

10.2 Lung design

10.3 Engineering small airways

10.4 Engineering alveolar structures

10.5 Conclusions

References

Chapter 11. Microfabricated Kidney Tissue Models

11.1 Introduction

11.2 Significance of microfabricated kidney tissue models

11.3 Kidney structure and function relationship

11.4 Traditional kidney tissue models

11.5 Crucial signaling elements for kidney tissue models

11.6 Review of current microfabricated kidney tissue models

11.7 Summary and future direction

References

Chapter 12. Microfluidic Cell Culture Techniques

12.1 Fundamentals of microscale cell culture

12.2 Microfluidic cell culture systems

12.3 Microenvironmental stimuli

12.4 Microfluidic cell and tissue culture systems for drug discovery and studies in physiology

12.5 Conclusions

Part 3: In Vitro Models

Chapter 13. Functionalized Microfluidic Devices for Separation of Cell Phenotypes

13.1 Introduction

13.2 Negative selection for enrichment of target cells

13.3 Positive selection of target cells for diagnostic purposes

13.4 Capture and release of target cells from positive selection for tissue engineering purposes

13.5 Effect of shear on changes of receptor expression in cells

13.6 Conclusions

References

Chapter 14. Microfluidic Hepatotoxicity Platform

14.1 Introduction

14.2 Liver tissue microenvironment

14.3 Microfluidic liver design

14.4 Long-term hepatocyte culture

14.5 Summary

Acknowledgments

References

Chapter 15. Live Cell Analysis Under Shear Flow

15.1 Introduction

15.2 Flow control and well plate microfluidics

15.3 Cell biology applications

15.4 Microbiology applications

15.5 Summary

References

Chapter 16. Microfluidic Platforms for Evaluating Angiogenesis and Vasculogenesis

16.1 Introduction

16.2 Current methods in microfluidics

16.3 Conclusion and future directions

References

Chapter 17. Cardiovascular Disease/Discovery Models

17.1 Introduction

17.2 Cell culture in cellix’s Vena8 Endothelial+microfluidic biochips

17.3 Microfluidic cell culture biochip model for atherosclerosis

17.4 Conclusion

References

Index