Microbial Cell Factories Engineering for Production of Biomolecules

Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others.

This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields.

Cover image
Title page
Table of Contents
Copyright
Dedication
Contributors
About the editor
Foreword
Preface
Acknowledgments
Chapter 1: An introduction to microbial cell factories for production of biomolecules
Abstract
Acknowledgments
1: Introduction
2: Microbial hosts as microbial cell factories
3: Design and optimization of microbial cell factories
4: Conclusion and future remarks
Chapter 2: Advances in long DNA synthesis
Abstract
1: Introduction, history, and evolution of gene synthesis
2: Technological developments
3: Applications of synthetic genes
4: Current challenges in DNA synthesis
5: Future developments
Chapter 3: Discovery of enzymes responsible for cyclization and postmodification in triterpenoid biosynthesis
Abstract
Acknowledgments
1: Introduction
2: Enzymes responsible for cyclization and postmodification of triterpenoid are crucial for triterpenoid biosynthesis
3: Approaches for discovery of enzymes in cyclization and postmodification of triterpenoid
4: Concluding remarks and future perspectives
Chapter 4: Synthetic biology approaches for secondary metabolism engineering
Abstract
1: Introduction
2: Secondary metabolism
3: Biosynthetic clusters for secondary metabolites
4: Top-down strategies: The known biology
5: Bottom-up strategies: De novo systems
6: Conclusions and further perspectives
Chapter 5: Synthetic biology design tools for metabolic engineering
Abstract
Acknowledgments
1: Introduction
2: Tools for metabolic modeling
3: Tools for metabolic pathway design
4: Tools for metabolic pathway experimental design
5: Tools for metabolic pathway dynamic regulation
6: Machine learning tools for metabolic pathway design
7: Automated design workflows and standardization
8: Conclusions and future perspectives
Chapter 6: Metabolic engineering for microbial cell factories
Abstract
1: Introduction
2: Metabolic engineering approaches
3: Emergence of systems metabolic engineering
4: Systems metabolic engineering strategies
5: The principles and tools for pathway prediction and design
6: The pathways constructed using rational and computational strategies
7: Metabolic flux analysis
8: Enhancing tolerance against products and inhibitors
9: Scale up and industrial production
10: Conclusion and future perspectives
Chapter 7: CRISPR-based tools for microbial cell factories
Abstract
Acknowledgments
1: Introduction
2: CRISPR-Cas editing at the single gene level
3: CRISPR-Cas editing at the genome level
4: Gene regulation tools
5: Conclusions
Chapter 8: Escherichia coli, the workhorse cell factory for the production of chemicals
Abstract
1: Introduction: Escherichia coli, a model microorganism for basic and applied research
2: Precursor biotransformation
3: De novo biotransformations and metabolic engineering
4: Concluding remarks
Chapter 9: Bacillus subtilis-based microbial cell factories
Abstract
Acknowledgments
1: Introduction
2: Bacillus as a workhorse for high-value compound biosynthesis
3: Engineered Bacillus subtilis
4: Use of coproducts for the synthesis of high-value chemicals
5: Minibacillus
6: Conclusion and future remarks
Chapter 10: Pseudomonas putida–based cell factories
Abstract
1: Introduction
2: Pseudomonas putida as a host for the production of natural products
3: Concluding remarks
Chapter 11: Streptomyces-based cell factories for production of biomolecules and bioactive metabolites
Abstract
1: Introduction
2: Streptomyces habitats
3: General characteristics of the genus Streptomyces
4: Growth requirements of Streptomyces species
5: Production of secondary metabolites
6: Streptomyces species as cell factories for production of antibiotics
7: Anticancer, immunostimulatory, immunosuppressive, and antioxidative agents produced by Streptomyces species
8: Streptomyces species as cell factories for production of active metabolites applied against causative agents of a number of diseases
9: Streptomyces species as cell factories for production of insecticides and antiparasitic agents
10: Streptomyces species as cell factories for production of a variety of enzymes
11: Streptomyces species as cell factories for production of bioemulsifiers and biosurfactants
12: Streptomyces species as cell factories for production of pigments
13: Streptomyces species as cell factories for synthesis of nanoparticles
14: Production of vitamins
15: Production of odors
16: Conclusion and future perspective
Chapter 12: Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications
Abstract
1: Introduction
2: Protein secretion system in C. glutamicum
3: C. glutamicum protein expression system
4: Gene editing tools applied in C. glutamicum
5: C. glutamicum as a major workhorse for production of small molecules
6: Conclusions
Chapter 13: Production of high value-added chemicals by engineering methylotrophic cell factories
Abstract
Acknowledgments
1: Introduction
2: New progress in genetic manipulation tools for engineering of MeCFs
3: Advances in engineering of the metabolic pathway in/from methylotrophs
4: Improvement of methylotrophic phenotypes via evolution
5: Producing high value-added chemicals by engineering MeCFs
6: Metabolic potential of native methylotrophs for synthesizing secondary metabolites
7: Conclusions and perspectives
Chapter 14: Cyanobacteria-based microbial cell factories for production of industrial products
Abstract
1: Introduction
2: Bioremediation
3: Biodiesel
4: Biohydrogen
5: Bioplastic
6: Microbial fuel cell
7: Nanoparticle synthesis by cyanobacteria
8: Exopolysaccharides producing cyanobacteria
9: Pigments producing cyanobacteria as microbial fuel cell
10: Carotenoids
11: 3-Phycobiliproteins
12: Phycocyanin
13: Phycoerythrin
14: Antiviral, antibacterial, antifungal, and anticancer compounds obtain by cyanobacteria
15: Conclusion and future perspectives
Chapter 15: Integrated omics perspective to understand the production of high-value added biomolecules (HVABs) in microalgal cell factories
Abstract
1: Introduction
2: Unraveling biosynthetic pathways for the production of HVABs
3: Integrated omics for the redesigning/remapping metabolic pathways for enhanced HVAB production
4: Microalgal cell factories: An overview
5: Conclusions and future remarks
Chapter 16: Saccharomyces cerevisiae as a microbial cell factory
Abstract
1: Introduction
2: Methods and applications for yeast transformation
3: Engineering of S. cerevisiae
4: Metabolic engineering of S. cerevisiae
5: Conclusion
Chapter 17: Pichia pastoris-based microbial cell factories
Abstract
1: Introduction
2: Genetic engineering tools for P. pastoris
3: Protein production by P. pastoris
4: Fermentative chemical production by P. pastoris
5: Chemical production by P. pastoris whole-cell biocatalyst
6: Conclusions
Chapter 18: Yarrowia lipolytica engineering as a source of microbial cell factories
Abstract
1: Introduction
2: Main characteristics of Yarrowia lipolytica
3: A short history of Yarrowia lipolytica use
4: Overview of basic tools for Yarrowia lipolytica engineering
5: A post–2010 era of new engineering technologies
6: Yarrowia lipolytica developing applications and future prospects
Chapter 19: Engineering of microbial cell factories for production of plant-based natural products
Abstract
1: Introduction
2: Host microorganisms
3: Metabolic engineering strategies in microorganisms for production of PNPs
4: Terpenoids
5: Alkaloids
6: Polyphenols
7: Conclusion and future challenges
Funding
Chapter 20: Engineering of microbial cell factories for omics-guided production of medically important biomolecules
Abstract
1: Introduction
2: Omics-driven bioproduction of medically important biomolecules and natural products
3: Conclusions and perspectives
Chapter 21: Advances and applications of cell-free systems for metabolic production
Abstract
1: Introduction
2: In vitro transcription-translation (TX-TL) systems
3: In vitro systems using purified enzymes
4: Applications of TX-TL systems for bioproduction
5: Conclusions and perspectives
Chapter 22: Microbial biosensors for discovery and engineering of enzymes and metabolism
Abstract
1: Introduction
2: Types and construction of microbial biosensors
3: Application of biosensors for the engineering of enzymes and metabolic pathways
4: Application of biosensors for the discovery of novel enzymes and metabolic pathways
5: Conclusions and perspectives
Chapter 23: Manipulation of global regulators in Escherichia coli for the synthesis of biotechnologically relevant products
Abstract
1: Introduction of Escherichia coli metabolism
2: Global regulation of metabolism
3: Control of the central carbon metabolism by global regulation
4: Manipulation of global regulators: Its effect on the synthesis of biotechnological compounds
5: Concluding remarks
Index

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Microbial Cell Factories Engineering for Production of Biomolecules

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Vijai Singh