Microbial Cell Factories Engineering for Production of Biomolecules
- 1st Edition - February 13, 2021
- Editor: Vijai Singh
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 1 4 7 7 - 0
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 1 4 7 8 - 7
Microbial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and techn… Read more
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Request a sales quoteMicrobial Cell Factories Engineering for Production of Biomolecules presents a compilation of chapters written by eminent scientists worldwide. Sections cover major tools and technologies for DNA synthesis, design of biosynthetic pathways, synthetic biology tools, biosensors, cell-free systems, computer-aided design, OMICS tools, CRISPR/Cas systems, and many more. Although it is not easy to find relevant information collated in a single volume, the book covers the production of a wide range of biomolecules from several MCFs, including Escherichia coli, Bacillus subtilis, Pseudomonas putida, Streptomyces, Corynebacterium, Cyanobacteria, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica, and algae, among many others.
This will be an excellent platform from which scientific knowledge can grow and widen in MCF engineering research for the production of biomolecules. Needless to say, the book is a valuable source of information not only for researchers designing cell factories, but also for students, metabolic engineers, synthetic biologists, genome engineers, industrialists, stakeholders and policymakers interested in harnessing the potential of MCFs in several fields.
- Offers basic understanding and a clear picture of various MCFs
- Explains several tools and technologies, including DNA synthesis, synthetic biology tools, genome editing, biosensors, computer-aided design, and OMICS tools, among others
- Harnesses the potential of engineered MCFs to produce a wide range of biomolecules for industrial, therapeutic, pharmaceutical, nutraceutical and biotechnological applications
- Highlights the advances, challenges, and future opportunities in designing MCFs
Researchers in field of metabolic engineering, genome engineering, synthetic biology and industrial microbiology
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Contributors
- About the editor
- Foreword
- Preface
- Acknowledgments
- Chapter 1: An introduction to microbial cell factories for production of biomolecules
- Abstract
- Acknowledgments
- 1: Introduction
- 2: Microbial hosts as microbial cell factories
- 3: Design and optimization of microbial cell factories
- 4: Conclusion and future remarks
- Chapter 2: Advances in long DNA synthesis
- Abstract
- 1: Introduction, history, and evolution of gene synthesis
- 2: Technological developments
- 3: Applications of synthetic genes
- 4: Current challenges in DNA synthesis
- 5: Future developments
- Chapter 3: Discovery of enzymes responsible for cyclization and postmodification in triterpenoid biosynthesis
- Abstract
- Acknowledgments
- 1: Introduction
- 2: Enzymes responsible for cyclization and postmodification of triterpenoid are crucial for triterpenoid biosynthesis
- 3: Approaches for discovery of enzymes in cyclization and postmodification of triterpenoid
- 4: Concluding remarks and future perspectives
- Chapter 4: Synthetic biology approaches for secondary metabolism engineering
- Abstract
- 1: Introduction
- 2: Secondary metabolism
- 3: Biosynthetic clusters for secondary metabolites
- 4: Top-down strategies: The known biology
- 5: Bottom-up strategies: De novo systems
- 6: Conclusions and further perspectives
- Chapter 5: Synthetic biology design tools for metabolic engineering
- Abstract
- Acknowledgments
- 1: Introduction
- 2: Tools for metabolic modeling
- 3: Tools for metabolic pathway design
- 4: Tools for metabolic pathway experimental design
- 5: Tools for metabolic pathway dynamic regulation
- 6: Machine learning tools for metabolic pathway design
- 7: Automated design workflows and standardization
- 8: Conclusions and future perspectives
- Chapter 6: Metabolic engineering for microbial cell factories
- Abstract
- 1: Introduction
- 2: Metabolic engineering approaches
- 3: Emergence of systems metabolic engineering
- 4: Systems metabolic engineering strategies
- 5: The principles and tools for pathway prediction and design
- 6: The pathways constructed using rational and computational strategies
- 7: Metabolic flux analysis
- 8: Enhancing tolerance against products and inhibitors
- 9: Scale up and industrial production
- 10: Conclusion and future perspectives
- Chapter 7: CRISPR-based tools for microbial cell factories
- Abstract
- Acknowledgments
- 1: Introduction
- 2: CRISPR-Cas editing at the single gene level
- 3: CRISPR-Cas editing at the genome level
- 4: Gene regulation tools
- 5: Conclusions
- Chapter 8: Escherichia coli, the workhorse cell factory for the production of chemicals
- Abstract
- 1: Introduction: Escherichia coli, a model microorganism for basic and applied research
- 2: Precursor biotransformation
- 3: De novo biotransformations and metabolic engineering
- 4: Concluding remarks
- Chapter 9: Bacillus subtilis-based microbial cell factories
- Abstract
- Acknowledgments
- 1: Introduction
- 2: Bacillus as a workhorse for high-value compound biosynthesis
- 3: Engineered Bacillus subtilis
- 4: Use of coproducts for the synthesis of high-value chemicals
- 5: Minibacillus
- 6: Conclusion and future remarks
- Chapter 10: Pseudomonas putida–based cell factories
- Abstract
- 1: Introduction
- 2: Pseudomonas putida as a host for the production of natural products
- 3: Concluding remarks
- Chapter 11: Streptomyces-based cell factories for production of biomolecules and bioactive metabolites
- Abstract
- 1: Introduction
- 2: Streptomyces habitats
- 3: General characteristics of the genus Streptomyces
- 4: Growth requirements of Streptomyces species
- 5: Production of secondary metabolites
- 6: Streptomyces species as cell factories for production of antibiotics
- 7: Anticancer, immunostimulatory, immunosuppressive, and antioxidative agents produced by Streptomyces species
- 8: Streptomyces species as cell factories for production of active metabolites applied against causative agents of a number of diseases
- 9: Streptomyces species as cell factories for production of insecticides and antiparasitic agents
- 10: Streptomyces species as cell factories for production of a variety of enzymes
- 11: Streptomyces species as cell factories for production of bioemulsifiers and biosurfactants
- 12: Streptomyces species as cell factories for production of pigments
- 13: Streptomyces species as cell factories for synthesis of nanoparticles
- 14: Production of vitamins
- 15: Production of odors
- 16: Conclusion and future perspective
- Chapter 12: Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications
- Abstract
- 1: Introduction
- 2: Protein secretion system in C. glutamicum
- 3: C. glutamicum protein expression system
- 4: Gene editing tools applied in C. glutamicum
- 5: C. glutamicum as a major workhorse for production of small molecules
- 6: Conclusions
- Chapter 13: Production of high value-added chemicals by engineering methylotrophic cell factories
- Abstract
- Acknowledgments
- 1: Introduction
- 2: New progress in genetic manipulation tools for engineering of MeCFs
- 3: Advances in engineering of the metabolic pathway in/from methylotrophs
- 4: Improvement of methylotrophic phenotypes via evolution
- 5: Producing high value-added chemicals by engineering MeCFs
- 6: Metabolic potential of native methylotrophs for synthesizing secondary metabolites
- 7: Conclusions and perspectives
- Chapter 14: Cyanobacteria-based microbial cell factories for production of industrial products
- Abstract
- 1: Introduction
- 2: Bioremediation
- 3: Biodiesel
- 4: Biohydrogen
- 5: Bioplastic
- 6: Microbial fuel cell
- 7: Nanoparticle synthesis by cyanobacteria
- 8: Exopolysaccharides producing cyanobacteria
- 9: Pigments producing cyanobacteria as microbial fuel cell
- 10: Carotenoids
- 11: 3-Phycobiliproteins
- 12: Phycocyanin
- 13: Phycoerythrin
- 14: Antiviral, antibacterial, antifungal, and anticancer compounds obtain by cyanobacteria
- 15: Conclusion and future perspectives
- Chapter 15: Integrated omics perspective to understand the production of high-value added biomolecules (HVABs) in microalgal cell factories
- Abstract
- 1: Introduction
- 2: Unraveling biosynthetic pathways for the production of HVABs
- 3: Integrated omics for the redesigning/remapping metabolic pathways for enhanced HVAB production
- 4: Microalgal cell factories: An overview
- 5: Conclusions and future remarks
- Chapter 16: Saccharomyces cerevisiae as a microbial cell factory
- Abstract
- 1: Introduction
- 2: Methods and applications for yeast transformation
- 3: Engineering of S. cerevisiae
- 4: Metabolic engineering of S. cerevisiae
- 5: Conclusion
- Chapter 17: Pichia pastoris-based microbial cell factories
- Abstract
- 1: Introduction
- 2: Genetic engineering tools for P. pastoris
- 3: Protein production by P. pastoris
- 4: Fermentative chemical production by P. pastoris
- 5: Chemical production by P. pastoris whole-cell biocatalyst
- 6: Conclusions
- Chapter 18: Yarrowia lipolytica engineering as a source of microbial cell factories
- Abstract
- 1: Introduction
- 2: Main characteristics of Yarrowia lipolytica
- 3: A short history of Yarrowia lipolytica use
- 4: Overview of basic tools for Yarrowia lipolytica engineering
- 5: A post–2010 era of new engineering technologies
- 6: Yarrowia lipolytica developing applications and future prospects
- Chapter 19: Engineering of microbial cell factories for production of plant-based natural products
- Abstract
- 1: Introduction
- 2: Host microorganisms
- 3: Metabolic engineering strategies in microorganisms for production of PNPs
- 4: Terpenoids
- 5: Alkaloids
- 6: Polyphenols
- 7: Conclusion and future challenges
- Funding
- Chapter 20: Engineering of microbial cell factories for omics-guided production of medically important biomolecules
- Abstract
- 1: Introduction
- 2: Omics-driven bioproduction of medically important biomolecules and natural products
- 3: Conclusions and perspectives
- Chapter 21: Advances and applications of cell-free systems for metabolic production
- Abstract
- 1: Introduction
- 2: In vitro transcription-translation (TX-TL) systems
- 3: In vitro systems using purified enzymes
- 4: Applications of TX-TL systems for bioproduction
- 5: Conclusions and perspectives
- Chapter 22: Microbial biosensors for discovery and engineering of enzymes and metabolism
- Abstract
- 1: Introduction
- 2: Types and construction of microbial biosensors
- 3: Application of biosensors for the engineering of enzymes and metabolic pathways
- 4: Application of biosensors for the discovery of novel enzymes and metabolic pathways
- 5: Conclusions and perspectives
- Chapter 23: Manipulation of global regulators in Escherichia coli for the synthesis of biotechnologically relevant products
- Abstract
- 1: Introduction of Escherichia coli metabolism
- 2: Global regulation of metabolism
- 3: Control of the central carbon metabolism by global regulation
- 4: Manipulation of global regulators: Its effect on the synthesis of biotechnological compounds
- 5: Concluding remarks
- Index
- No. of pages: 488
- Language: English
- Edition: 1
- Published: February 13, 2021
- Imprint: Academic Press
- Paperback ISBN: 9780128214770
- eBook ISBN: 9780128214787
VS
Vijai Singh
Dr Vijai Singh is a Professor and Dean (Research & Innovation) at School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Associate Professor in the Department of Biosciences, School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. Prior this this, he was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 11 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 100 articles, 70 chapters, 15 books and 3 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University.