Methods of Animal Experimentation

List of Contributors

Preface

Contents of Volume I

Introduction

Chapter 1 Radiation Hygiene

I. The Nature and Assessment of Ionizing Radiation Effects

II. Health Physics

Appendix

References

Chapter 2 Use of Ionizing Radiation for Measuring Biological Phenomena

I. Introduction

II. Laboratory Design

III. Handling of Radioisotopes in the Animal Colony

IV. Administration of Radiation or Radioisotopes to the Experimental Animal

V. Collection of Samples

VI. Uses of Radioisotopes

Appendix

References

Chapter 3 Tumor Transplantation

I. Introduction

II. History and Biological Properties of 38 Transplantable Mouse, Rat, Hamster, and Chicken Tumors

III. Techniques of Tumor Transplantation

IV. Importance of Uniform Inoculum

V. Importance of Animal Strain

VI. Inoculation Site

VII. Influence of Age and Sex

VIII. Influence of Health

IX. Influence of Pregnancy

X. Interval after Which Growth Becomes Apparent

XI. Comparative Growth Rate of the Malignant Cells

XII. Inoculation of Stationary or Receding Tumors

XIII. Spontaneous Regression of Transplantable Tumors

XIV. Resistance to Reinoculation

XV. Frozen Storage of Transplantable Tumors

XVI. Summary

References

Chapter 4 Parabiosis

I. Introduction

II. Methods

III. Blood and Fluid Exchange Between Parabiotic Rats

IV. Diseases of Parabiosis

References

Chapter 5 Organ Transplantation

I. General Discussion of the Types of Problem That Can Be Investigated by Experimental Organ Transplantation

II. The Selection of Animals for Organ Transplantation

III. The Surgical Technique

IV. Methods of Assessment of Renal Function and Homograft Reaction in Animals with Renal Transplants

V. Summary

References

Chapter 6 The Care and Use of Hibernating Mammals

I. Hibernation

II. Mammals That Hibernate

III. Types of Mammalian Hibernation

IV. Environmental Factors

V. Hibernation versus Hypothermia

VI. Estivation

VII. Choice of Animal for Research

VIII. Procurement of Animals

IX. Care of Individual Species

X. Techniques for Use of Hibernators

References

Chapter 7 High-Altitude Studies

I. Introduction

II. "Natural" High-Altitude Studies

III. "Simulated" High-Altitude Studies

IV. Conclusions

References

Chapter 8 Care and Growth of Animals during Chronic Centrifugation

I. Introduction

II. Applications

III. General Considerations in the Design of Centrifuge Programs

IV. Specific Procedures Employed with Experimental Animals

V. Procedures of Measurement and Analysis

VI. Summary

References

Chapter 9 Controlled Exercise

I. Introduction

II. Methods of Inducing Controlled Exercise

III. Factors Influencing Controlled Exercise Results

IV. Factors Affecting Endurance

VI Influence of Excess Exercise on Various Animal Characteristics

VI. Summary

References

Chapter 10 Methods of Animal Infection with Bacteria, Fungi, and Viruses

I. Introduction

II. Animal Inoculation versus Tissue Culture Testing and the Use of Artificial Media

III. Factors Influencing the Choice of Animals and Route of Inoculation

IV. General Procedures According to Route of Inoculation

V. Study of Specific Infections

References

Chapter 11 Electronic Monitoring of Physiological Phenomena in Experimental Animals

I. Components of a Monitoring System

II. Electrocardiography

III. Intravascular and Intracardiac Electrocardiography

IV. Phonocardiography

V. Intravascular Pressures

VI. Ultrasound

VII. Temperature Monitoring

VIII. Respiration

IX. Densitometry

X. Electrophysiological Signals from the Brain

References

Author Index

Subject Index