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Methods for Plant Molecular Biology
- 1st Edition - September 24, 2013
- Editors: Arthur Weissbach, Herbert Weissbach
- Language: English
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 6 9 7 2 - 6
Methods for Plant Molecular Biology is a collection of articles that focuses on the techniques used in plant molecular biology and genetics. The book discusses the isolation and… Read more
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Request a sales quoteMethods for Plant Molecular Biology is a collection of articles that focuses on the techniques used in plant molecular biology and genetics. The book discusses the isolation and characterization of nuclear, chloroplast, and mitochondrial nucleic acids and the factors and systems involved in transcription and gene expression. Procedures for the isolation of cell walls, chloroplast membranes, membrane proteins; techniques to carry out plant cell culture and protoplast formation; and methods for gene and organelle transfer are covered as well. Biologists, molecular biologists, botanists, and students will find the book very useful.
Table of Contents
List of Contributors
Preface
Contents of Methods in Enzymology,Volume 118
Section I. Cell Wall and Membrane
1. Isolation and Characterization of Plant Cell Walls and Cell Wall Components
2. Isolation of the Plasma Membrane
Section II. Nucleus
3. Purification and Restriction Endonuclease Analysis of Plant Nuclear DNA
4. Analyzing Genome Variation in Plants
Section III. Cytoplasmic Protein Synthesis
5. The Synthesis of High-Molecular-Weight Proteins in the Wheat Germ Translation System
Section IV. The Chloroplast
A. Genome Structure
6. Isolation and Structural Analysis of Chloroplast
B. Transcription-Translation
7. In Vitro Transcription of Chloroplast Protein Genes
8. Coupled Transcription-Translation in Chloroplast
9. Translation by Isolated Pea Chloroplasts
C. Photosynthetic Systems
10. Isolation and Characterization of Chloroplast Envelope Membranes
11. Characterization of the Thylakoid Membrane by Subfractionation Analyses
12. Subunit Structure and Biogenesis of ATP Synthase and Photosystem I Reaction Center
D. RuBP Carboxylase
13. Cloning and Expression of Genes for the Small Subunit of Ribulosebisphosphate Carboxylase
14. Separation and Reassembly of the Subunits of Ribulosebisphosphate Carboxylase
15. The Cloning and Expression in Escherichia coli of RuBP Carboxylase/Oxygenase Large Subunit Genes
Section V. Mitochondria
16. The Isolation of Mitochondria and Mitochondrial
17. Analysis of the Genome Structure of Plant Mitochondria
18. Strategies for the Identification and Analysis of Higher Plant Mitochondrial Genes
19. Isolation of Plant Mitochondrial
Section VI. Nitrogen Metabolism
20. Isolation and Characterization of Nitrogenase from Klebsiella pneumonia
Section VII. Cell Culture and Transformation
22. Establishment of Calli and Suspension Cultures
23. Protoplasts: Isolation, Culture, Plant Regeneration
24. Fusion and Transformation of Plant Protoplasts
25. Organelle Transfer
Section VIII. Gene Transfer
26. Gene Transfer in Plants: Production of Transformed Plants Using Ti Plasmid Vectors
27. Plant Virus Vectors: Cauliflower Mosaic Virus
28. Direct Gene Transfer to Plants
Section IX. Virology
29. In Vitro Transcription of Infectious Viral RNA from Cloned cDNA
30. Use of RNA Probes to Detect Plant RNA Viruses
31. Preparation and Use of cDNA Probes for Detection of Viral Genomes
32. ELISA Techniques
Subject Index
- No. of pages: 564
- Language: English
- Edition: 1
- Published: September 24, 2013
- Imprint: Academic Press
- eBook ISBN: 9781483269726
AW
Arthur Weissbach
Affiliations and expertise
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey, U.S.A.HW
Herbert Weissbach
Affiliations and expertise
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey, U.S.A.Read Methods for Plant Molecular Biology on ScienceDirect