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Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 67th volume, the series features severa… Read more
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Contributors
Chapter One. High-Resolution 1H-NMR Spectroscopy and Beyond to Explore Plant Metabolome
1 Introduction
2 NMR Signals: Measurable Properties
3 Metabolomics NMR Fingerprinting for Discriminating Plant Metabolomes
4 Metabolomics NMR Profiling and Spectra Annotation
5 Application of 1H-NMR Profiling
6 New Challenges for 1H-NMR Metabolomics
7 Concluding Remarks
References
Chapter Two. Application of LC–MS and LC–NMR Techniques for Secondary Metabolite Identification
1 Introduction
2 Principal Secondary Metabolites in Plants
3 Hyphenated Chromatographic Techniques in Metabolite Identification
4 LC–NMR Techniques
5 Combination of LC–MS and LC–NMR in Metabolite Profiling
6 Conclusion
References
Chapter Three. Fast and Ultrafast Quantitative 2D NMR: Vital Tools for Efficient Metabolomic Approaches
1 Introduction
2 Why Quantitative 2D NMR?
3 Fast Conventional 2D NMR Approaches for Quantitative Analysis of Complex Mixtures
4 Going Faster
5 Conclusion
Acknowledgements
References
Chapter Four. Liquid Chromatography Coupled to Mass Spectrometry-Based Metabolomics and the Concept of Biomarker
1 Introduction
2 LC/MS-Based Metabolomics for Biomarker Discovery
3 Conclusion and Perspectives
Acknowledgements
References
Chapter Five. Potential of Fourier Transform Mass Spectrometry for High-Throughput Metabolomics Analysis
1 Introduction
2 Definition of Some Concepts Used in Mass Spectrometry
3 Principle of Fourier Transform Mass Spectrometers
4 Ion Production for Metabolite Analysis
5 FT-MS Applications for Complex Mixture Analysis
6 Metabolite Identification from HRMS Data
7 Conclusion
Acknowledgements
References
Chapter Six. Exploring Metabolome with GC/MS
1 Introduction
2 Sample Extraction for Metabolomic Analysis
3 GC/MS Profiling
4 Data Processing
5 Quantification
6 Applications
7 Conclusion
References
Chapter Seven. The Lipid World Concept of Plant Lipidomics
Abbreviations
1 Introduction
2 The Diversity of Plant Lipids
3 Lipid Extraction
4 Sample Storage
5 Principles of MS Lipid Analysis
6 Plant Lipid Analyses
7 Conclusion
Acknowledgements
References
Chapter Eight. Isotope Ratio Mass Spectrometry Technique to Follow Plant Metabolism: Principles and Applications of 12C/13C Isotopes
Abbreviations
1 Stable Isotopes at Natural Abundance
2 Definitions
3 Basics of Photosynthetic 12C/13C Discrimination
4 Practical Aspects of EA-IRMS Measurements
5 Compound-Specific IRMS Analyses
6 Major Advances in Metabolic Patterns Provided by IRMS Techniques
7 Conclusions and Perspectives
References
Chapter Nine. High-Throughput Biochemical Phenotyping for Plants
1 Introduction
2 Biochemical Phenotyping in Medicine, Agro Industry and Agriculture
3 Solutions for a Microplate Pipeline Dedicated to Plant Biochemical Phenotyping
4 Example of HT Biochemical Screen
5 Concluding Remarks
Acknowledgements
References
Chapter Ten. Two Computational Simplex Approaches to Graphical Highlighting Metabolic Phenotypes and Their Functional Origins: Correspondence Analysis and Weighted Metabolic Profiles Analysis
1 Introduction
2 Geometrical and Numerical Bases of Simplex Space
3 CA: Principle and Method
4 Weighted Metabolic Profiles Analysis
5 Application Example Combining CA and WMPA
References
Chapter Eleven. Strategies for Data Handling and Statistical Analysis in Metabolomics Studies
1 Introduction
2 General Characteristics of the Data
3 NMR Data Recording and Preprocessing: The Necessary Steps Prior to Statistical Analyses
4 LC/MS Data Recording and Preprocessing: The Necessary Steps Prior to Statistical Analyses
5 GC/MS Data Recording and Preprocessing: The Necessary Steps Prior to Statistical Analyses
6 Data Pretreatment
7 Basic Methods for Statistical Analysis: Possible Approaches
8 Reproducibility and Experimental Issues
9 Supervised Methods for Classification Problems: Potential for Statistical Bias
References
Chapter Twelve. Qualitative Modelling of Metabolic Networks
1 Introduction
2 Reconstruction, Storage and Sharing
3 Graph Formalism for Network Mining
4 Visualization of Metabolic Networks
5 Conclusion
References
Chapter Thirteen. Modelling Metabolic Networks—The Theories of Metabolism
1 Which Rate Equations for the Reactions of a Metabolic Network?
2 The Structure of Metabolic Network: The Stoichiometry Matrix
3 The Steady State
4 The Structure of Metabolic Network: The Elementary Flux Modes (efms)
5 Metabolic Control Analysis
6 Softwares and Tools for Kinetic Modelling
7 Conclusion: Protocol for the Study of Metabolic Networks at Steady State
References
Author Index
Subject Index