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Membrane Protein Purification and Crystallization

A Practical Guide

  • 2nd Edition - October 13, 1980
  • Latest edition
  • Editors: Carola Hunte, Gebhard von Jagow, Hermann Schagger
  • Language: English

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and pr… Read more

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Description

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Key features

  • Provides general guidelines and strategies for isolation and crystallization of membrane proteins
  • Gives detailed protocols that have wide application, and low specialized equipment needs
  • Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins
  • Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes
  • Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization
  • Presents a comprehensive guide to 2D-crystallization of membrane proteins

Readership

Biochemists, molecular, cell, and structural biologists, and researchers in the pharmaceutical and biotechnical industry

Table of contents

I. Strategies and Techniques
Purification Strategies for Membrane Proteins
Chromatographic Techniques and Basic Operations in Membrane Protein Purification
Production and purification of recombinant membrane proteins
SDS Electrophoresis Techniques
Blue-Native Electrophoresis
Preparative Isoelectric Focussing
Membrane protein crystallization

II. Discussion of Selected Isolation Protocols
Lipid Dependent Inactivation and Reactivation of Bovine complex III
Purification of affinity-epitope tagged G-protein coupled receptors
Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D
Crystallization
Purification of the yeast cytochrome bc1 complex

III. Crystallization of Membrane Proteins
Antibody fragment-mediated crystallization of membrane proteins
Crystallization of Wolinella succinogenes quinol:fumarate reductase
Ba3-type cytochrome-c oxidase from Thermus thermophilus
purification, crystallization, and crystal transformation
Two-dimensional crystallization of membrane proteins: a practical guide
In cubo crystallization of membrane proteins

Product details

  • Edition: 2
  • Latest edition
  • Published: December 19, 2002
  • Language: English

About the editors

CH

Carola Hunte

Affiliations and expertise
Max-Planck-Institut fuer Biophysik, Frankfurt, Germany

Gv

Gebhard von Jagow

Affiliations and expertise
Zentrum Für Biologische Chemie, Frankfurt, Germany

HS

Hermann Schagger

Deceased: February 12, 2017
Affiliations and expertise
Zentrum Für Biologische Chemie, Universitätsklinikum Frankfurt, Germany

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