Livestock Genome Editing Tools

1st Edition - April 13, 2024
Editors: Kui Li, Bingyuan Wang
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 1 9 0 9 9 - 9
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 1 9 1 0 0 - 2

Livestock Genome Editing Tools introduces applications and improvements to a series of new genome editing techniques in livestock, such as pigs, cattle, and sheep. These tools… Read more

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Livestock Genome Editing Tools introduces applications and improvements to a series of new genome editing techniques in livestock, such as pigs, cattle, and sheep. These tools include zinc finger nucleases (ZFN), transcription activator–like effector nucleases (TALEN), and the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system, as well as the traditional gene targeting tools. The book also summarizes a series of genome editing–related techniques, including microinjection and somatic cell nuclear transfer.

Written by international experts who have been working on livestock genetic editing field for more than 30 years, this book provides extensively theoretical and practical experience for readers to master the latest developments. This book explores the importance of research and application, as well as operation procedures, of livestock genetic editing tools. The writing of operation details makes this book an accessible read.

This book is an important resource for researchers interested in genome-edited animals and scientists and technicians in breeding institutions and is also of interest to students major in animal reproduction and biological engineering.

Cover image
Title page
Table of Contents
Copyright
List of contributors
About the editors
Preface
Chapter 1. The development of genome editing technology in animals
Abstract
1.1 Introduction
1.2 Basic process of generating genetically engineered animals by gene targeting
1.3 Factors affecting the targeting efficiency
1.4 Traditional gene targeting technology
1.5 Application of gene targeting technology
References
Chapter 2. New tools for genome editing
Abstract
2.1 Zinc finger nucleases
2.2 Transcription activator-like effector nucleases
2.3 CRISPR/Cas9 system
2.4 Base editing
References
Chapter 3. Associated technologies for genome editing
Abstract
3.1 Pronuclear microinjection
3.2 Cytoplasmic injection
3.3 Somatic cell nuclear transfer technology
3.4 Electroporation
3.5 RNA interference technology
3.6 Transgenic technology of viral vectors
3.7 Artificial chromosome technology
3.8 Transposons
3.9 Sperm-mediated gene transfer technology
3.10 Preparation techniques for the complex traits of transgenic animals
References
Chapter 4. Application status of genome-editing tools in pig
Abstract
4.1 Introduction
4.2 Research and development of pig-friendly loci and efficient editing technology system
4.3 Increasing meat production
4.4 Disease resistance breeding
4.5 Environment protection
4.6 Xenotransplantation
4.7 Human disease model
4.8 Production of medicinal protein
4.9 Prospection
References
Chapter 5. Application state of genome-editing tools in cattle
Abstract
5.1 Research progress toward genome-edited cattle
5.2 Conclusions and prospects
5.3 Vector construction and function verification
5.4 Genome-edited somatic cell nuclear transfer
5.5 Embryo transfer and pregnancy management
5.6 Genome-edited mice as a functional verification model for cattle genome editing
5.7 Optimized pronuclear injection for genome-edited mouse production
References
Chapter 6. Application status of genome-editing tools in sheep and goats
Abstract
6.1 Current status on application of genome editing in sheep and goats
6.2 Progress in research and development of sheep gene editing
6.3 The technical process of sheep gene editing
6.4 Prospect of gene editing in sheep
References
Chapter 7. Evaluation and detection of genome-edited livestock
Abstract
7.1 Biosafety evaluation of genetically engineered animals
7.2 Molecular characterization of genetically engineered animals
7.3 A framework for safety assessment of genome-edited animals
References
Chapter 8. Breeding by molecular writing: the future development of livestock breeding
Abstract
8.1 Current situation and difficulties of animal breeding
8.2 The content and research status of breeding by molecular writing
8.3 Process of breeding by molecular writing
References
Index

Kui Li

Dr. Kui Li is a Professor at Chinese Academy of Agricultural Sciences. He graduated from Wuhan University of China and appointed as a full Professor at Huazhong (Central China) Agricultural University and Chinese Academy of Agricultural Sciences in China since 1996, respectively. He used to be a visiting scholar at the University of Sydney, Australia and a full-time visiting Professor at the Cornell University, USA. Prof. Li’s research interests include pig genome and breeding, pig genetic engineering, experimental mini-pigs and medical models. He has been working on these fields for more than 3 decades. Since 1996, Prof. Li systematically compared the multi-omics of different developmental stages of Chinese and Western pig breeds and found lots of multi-level molecular markers. He also identified several hundreds of molecular markers related to porcine economic traits which are used to develop pig breeds. He established steady platforms for porcine somatic cell nuclear transfer, pronuclear injection, and genome editing techniques. He also established the techniques to develop genetic modified pigs with multi-gene compound traits, controllable expression and friendly gene locus site directed integration. Excitingly, Prof. Li’s research group generated more than 20 new breeding materials for agriculture including MSTN-edited pigs, anti-porcine reproductive and respiratory syndrome pigs. His research group also generated genome edited pigs for biomedicine including cardiovascular disease and diabetes models, which showed great application perspective. Prof Li is a founder of Agsino Gensources Co., Ltd., an animal biotechnology breeding company.

Affiliations and expertise

Dr. Kui Li is a professor at the Chinese Academy of Sciences.

Bingyuan Wang

Dr. Bingyuan Wang is an Associate Professor at the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences. In 2014, she graduated from Max Planck Institute for Molecular Biomedicine in Germany. From 2015 to 2023, she worked in the Porcine Gene Engineering and Plasma Innovation group, which is led by Prof. Kui Li. Her research interests include the molecular mechanisms underlying porcine spermatogonial cells proliferation and apoptosis, the influence of exogenous factors in the porcine reproduction using porcine oocytes and spermatogonial cells, induction of gametes from porcine spermatogonial cells, and the generation of genome-edited pigs using porcine spermatogonial cells.

Affiliations and expertise

Research Associate, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health