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Lipid Droplets
- 1st Edition, Volume 116 - October 4, 2013
- Editors: H. Robert Yang, Peng Li
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 4 0 8 0 5 1 - 5
- eBook ISBN:9 7 8 - 0 - 1 2 - 4 0 9 5 2 1 - 2
This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging a… Read more
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Request a sales quoteThis new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field.
With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come.
- Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth
- Chapters are written by experts in the field
- Cutting-edge material
Researchers and students in cell, molecular and developmental biology
Series Page
Contributors
Preface
Chapter 1. Proteomic Studies of Isolated Lipid Droplets from Bacteria, C. elegans, and Mammals
Abstract
Introduction
Acknowledgment
References
Chapter 2. Analysis of Yeast Lipid Droplet Proteome and Lipidome
Abstract
Introduction
2.1 Materials
2.2 Methods
2.3 Results and Discussion
2.4 Notes
Acknowledgments
References
Chapter 3. Visualization of Lipid Droplets in C. elegans by Light and Electron Microscopy
Abstract
Introduction
Summary
Acknowledgments
References
Chapter 4. Analysis of Lipid Droplet Dynamics and Functions in Drosophila melanogaster
Abstract
4.1 Genome-Wide RNA Interference Screens in Drosophila Cells to Identify Regulators for LD Biology
4.2 LD Dynamics in Different Drosophila Developmental Stages
4.3 LD Functions in Drosophila Physiology
Acknowledgments
References
Chapter 5. Analysis of Oil Droplets in Microalgae
Abstract
Introduction
5.1 Nile Red as a Probe for Oil Droplets
5.2 Confocal and Transmission Electron Microscopic Observation
5.3 Isolation and Purification of Oil Droplets
Summary
Acknowledgments
References
Chapter 6. Studying Lipolysis in Adipocytes by Combining siRNA Knockdown and Adenovirus-Mediated Overexpression Approaches
Abstract
Introduction and Rationale
6.1 Materials and Reagents
6.2 Methods
Concluding Remarks
Acknowledgment
References
Chapter 7. Analysis of Lipid Droplets in Hepatocytes
Abstract
Introduction and Rationale
7.1 Materials
7.2 Methods
7.3 Discussion
Conclusion
Acknowledgments
References
Chapter 8. Analysis of Lipid Droplets in Cardiac Muscle
Abstract
Introduction and Rationale
8.1 Isolation of Cardiac LD Proteins and Preparation of Cardiac LDs for Analysis by Western Blot
8.2 Analysis of Cardiac LDs by 2D Analysis of LDs by Conventional TEM
8.3 Progress in 3D EM Technology May Provide New Tools to Study Cardiac LDs and Mitochondria Interactions
Conclusions
Acknowledgments
References
Chapter 9. Imaging Cytoplasmic Lipid Droplets in Enterocytes and Assessing Dietary Fat Absorption
Abstract
Introduction and Rationale
9.1 Materials and Methods
9.2 Discussion
Acknowledgment
References
Chapter 10. Lipid Droplets and Viral Infections
Abstract
Introduction
10.1 Materials
10.2 Methods
10.3 Discussion
Acknowledgments
References
Chapter 11. Purification of Integral Membrane Proteins and Lipid-Binding Assays
Abstract
Introduction and Rationale
11.1 Materials
11.2 Methods
Conclusion and Applications
Acknowledgment
References
Further Reading
Chapter 12. Imaging of Neutral Lipids and Neutral Lipid Associated Proteins
Abstract
Introduction and Rationale
12.1 Materials
12.2 Methods
12.3 Discussion
Summary
Acknowledgments
References
Chapter 13. Imaging Lipid Droplets by Electron Microscopy
Abstract
Introduction
13.1 Probing the LD Core
13.2 Observation of the LD Surface
13.3 Freeze-Substitution
13.4 Immunoelectron Microscopy of LD-Associated Proteins
13.5 Freeze-Fracture Electron Microscopy
13.6 Future directions
Acknowledgments
References
Chapter 14. Imaging Lipid Droplet Fusion and Growth
Abstract
Introduction
Conclusions
Acknowledgments
References
Index
Volumes in Series
- No. of pages: 270
- Language: English
- Edition: 1
- Volume: 116
- Published: October 4, 2013
- Imprint: Academic Press
- Hardback ISBN: 9780124080515
- eBook ISBN: 9780124095212
HY
H. Robert Yang
Affiliations and expertise
Faculty of Science, University of South Wales, AustraliaPL
Peng Li
Affiliations and expertise
School of Life Sciences, Tsinghua University, ChinaRead Lipid Droplets on ScienceDirect