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Laboratory Procedures for Veterinary Technicians
- 7th Edition - December 13, 2018
- Author: Margi Sirois
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 5 9 5 3 8 - 4
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 5 9 5 0 3 - 2
**Selected for Doody’s Core Titles® 2024 with "Essential Purchase" designation in Veterinary Nursing & Technology**Ensure you’re at your clinical best! Laboratory Procedures… Read more
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Request a sales quote**Selected for Doody’s Core Titles® 2024 with "Essential Purchase" designation in Veterinary Nursing & Technology**
Ensure you’re at your clinical best! Laboratory Procedures for Veterinary Technicians, 7th Edition covers the broad spectrum of laboratory procedures that veterinary technicians need to perform effectively in the practice setting. Comprehensive content presents the fundamentals of microbiology, hematology, urinalysis, immunology, and cytology, along with the laboratory procedures used to perform the most widely used tests such as complete blood count, urinalysis, and immunologic assays. This thoroughly updated edition includes an expanded Quality Control and Record Keeping chapter along with the latest advances in veterinary clinical procedures to prepare you for real-life laboratory work.
- Comprehensive coverage gives you a solid foundation in the fundamentals of microbiology, hematology, urinalysis, immunology, and cytology, along with the laboratory procedures used to perform related tests.
- Provides the latest information needed to successfully perform a broad spectrum of laboratory tests, including complete blood count, urinalysis, and immunologic assays.
- Step-by-step procedure boxes offer quick access to the skills you must perform during your educational program, as well as procedures that are commonly performed by vet techs in private practice.
- A comprehensive glossary of terms at the end of the text offers accurate, concise definitions.
- Vet Tech Threads provide you with introductions, suggested readings, boxed technician notes, learning objectives, chapter outlines, key terms, and a glossary for easy navigation through chapters and more focused learning.
- Cover image
- Title Page
- Table of Contents
- IFC
- Procedures
- Copyright Page
- Dedication
- Contributors
- Preface
- Acknowledgments
- Unit 1 The Veterinary Practice Laboratory
- Introduction
- 1 Safety Concerns and OSHA Standards
- Hazard Control
- OSHA Standards
- Laboratory Design
- Review Questions
- Key Points
- 2 General Laboratory Equipment
- Test Tubes
- Centrifuge
- Refractometer
- Pipettes
- Temperature-Controlling Equipment
- Automated Analyzers
- Miscellaneous Equipment and Supplies
- Review Questions
- Key Points
- 3 The Microscope
- Care and Maintenance
- Calibration of the Microscope
- Digital Microscopy
- Review Questions
- Key Points
- 4 The Metric System and Lab Calculations
- Numbering Systems
- Review Questions
- Key Points
- 5 Quality Control and Record Keeping
- Accuracy, Precision, and Reliability
- Analysis of Control Materials
- Errors
- Applied Quality Control
- Laboratory Records
- Review Questions
- Key Points
- Unit 2 Hematology
- Introduction
- 6 Hematopoiesis
- Hematopoiesis
- Erythropoiesis
- Thrombopoiesis
- Granulopoiesis
- Monopoiesis
- Lymphopoiesis
- Definitions
- Review Questions
- Key Points
- 7 Sample Collection and Handling
- Collection and Handling of Blood Samples
- Sample Volume
- Collection Procedure
- Review Questions
- Key Points
- 8 Automated Analyzers
- Cell Counts
- Types of Hematology Instruments
- Review Questions
- Key Points
- 9 Hemoglobin, PCV, and Erythrocyte Indices
- Packed Cell Volume
- Hemoglobin Testing
- Erythrocyte Indices
- Review Questions
- Key Points
- 10 Evaluating the Blood Smear
- Preparation of Blood Smears
- Review Questions
- Key Points
- 11 Morphologic Abnormalities of Blood Cells
- Quantifying Morphologic Changes
- Morphologic Abnormalities Seen in White Blood Cells
- Morphologic Abnormalities Seen in Red Blood Cells
- Review Questions
- Key Points
- 12 Additional Hematologic Tests
- Reticulocyte Count
- Bone Marrow Evaluation
- Erythrocyte Sedimentation Rate
- Review Questions
- Key Points
- 13 Hematopoietic Disorders and Classification of Anemia
- Disorders of the Bone Marrow
- Classification of Anemia
- Review Questions
- Key Points
- Unit 3 Hemostasis
- Introduction
- 14 Principles of Blood Coagulation
- Overview of Blood Coagulation
- Coagulation Testing
- Review Questions
- Key Points
- 15 Sample Collection and Handling
- Sample Collection and Handling
- Coagulation Instrumentation
- Review Questions
- Key Points
- 16 Platelet Evaluation
- Platelet Count
- Review Questions
- Key Points
- 17 Coagulation Testing
- Buccal Mucosa Bleeding Time
- Activated Clotting Time
- Whole Blood Clotting Time
- Activated Partial Thromboplastin Time
- Prothrombin Time Test
- Clot Retraction Test
- Fibrinogen Determination
- PIVKA
- D-Dimer and Fibrin Degradation Products
- Von Willebrand Factor
- Coagulation Factor Assays
- Review Questions
- Key Points
- 18 Disorders of Hemostasis
- Hemostatic Defects
- Hereditary Coagulation Disorders
- Acquired Coagulation Disorders
- Disseminated Intravascular Coagulation
- Review Questions
- Key Points
- Unit 4 Immunology
- Introduction
- 19 Basic Principles of Immunity
- Innate Immune System
- Adaptive Immune System
- Passive Immunity
- Immunization
- Review Questions
- Key Points
- 20 Common Immunologic Laboratory Tests
- Sample Collection and Handling
- Handling Serologic Samples
- Tests of Humoral Immunity
- Immunology Analyzers
- Review Questions
- Key Points
- 21 Blood Groups and Immunity
- Blood Types
- Blood Typing
- Crossmatching
- Review Questions
- Key Points
- 22 Intradermal Testing
- Tests of Cell-Mediated Immunity
- Review Questions
- Key Points
- 23 Reference Laboratory Immunoassays
- Coombs Testing
- Immunodiffusion
- Radioimmunoassay
- Fluorescent Antibody Testing
- Antibody Titers
- Molecular Diagnostics
- Review Questions
- Key Points
- 24 Disorders of the Immune System
- Hypersensitivity
- Review Questions
- Key Points
- Unit 5 Urinalysis
- Introduction
- 25 Anatomy and Physiology of the Urinary System
- Formation of Urine
- Urine Volume Regulation
- Review Questions
- Key Points
- 26 Sample Collection and Handling
- Voided or Free-Catch Samples
- Bladder Expression
- Catheterization
- Cystocentesis
- Specimen Storage and Handling
- Review Questions
- Key Points
- 27 Physical Examination of Urine
- Urine Volume
- Color
- Clarity/Transparency
- Odor
- Specific Gravity
- Review Questions
- Key Points
- 28 Chemical Evaluation
- pH
- Protein
- Glucose
- Ketones
- Bile Pigments
- Blood (Hemoprotein)
- Leukocytes
- Urinalysis Analyzers
- Review Questions
- Key Points
- 29 Urine Sediment Analysis
- Dry-Mount Urine Cytology
- Constituents of Urine Sediment
- Microorganisms
- Miscellaneous Components of Urine
- Urolithiasis
- Review Questions
- Key Points
- Unit 6 Clinical Chemistry
- Introduction
- 30 Sample Collection and Handling
- Plasma
- Serum
- Factors That Influence Results
- Reference Ranges
- Review Questions
- Key Points
- 31 Automated Analyzers
- Photometry
- End Point Versus Kinetic Assays
- Units of Measurement
- Ion-Selective Electrode and Electrochemical Methods
- Features and Benefits of Common Chemistry Analyzer Types
- Instrument Care and Maintenance
- Review Questions
- Key Points
- 32 Protein Assays and Hepatobiliary Function Tests
- Protein Assays
- Hepatobiliary Assays
- Review Questions
- Key Points
- 33 Kidney Function Tests
- Blood Urea Nitrogen
- Serum Creatinine
- Blood Urea Nitrogen/Creatinine Ratio
- Urine Protein/Creatinine Ratio
- Uric Acid
- Tests of Glomerular Function
- Review Questions
- Key Points
- 34 Pancreatic Function Tests
- Exocrine Pancreas Tests
- Endocrine Pancreas Tests
- Review Questions
- Key Points
- 35 Electrolytes and Acid–Base Status
- Acid–Base Balance
- Acidosis and Alkalosis
- Electrolyte Assays
- Review Questions
- Key Points
- 36 Miscellaneous Tests
- Creatine Kinase
- Troponin and Brain Natriuretic Peptide
- Lactate
- Endocrine System Assays
- Chemical Tests of Gastrointestinal Function
- Toxicology
- Review Questions
- Key Points
- Unit 7 Microbiology
- Introduction
- 37 Introduction to Microbiology
- Bacterial Cell Morphology
- Bacterial Growth
- Virology
- Review Questions
- Key Points
- 38 Equipment and Supplies
- The In-House Microbiology Laboratory
- Laboratory Safety
- Equipment and Supplies Needed for the Microbiology Laboratory
- Culture Media
- Review Questions
- Key Points
- 39 Sample Collection and Handling
- General Guidelines
- Collection of Viral Specimens
- Review Questions
- Key Points
- 40 Staining Specimens
- Gram Stain
- Potassium Hydroxide Test
- Ziehl-Neelsen Stain
- Giemsa Stain
- Specialized Stains
- Quality Control
- Review Questions
- Key Points
- 41 Culture Techniques
- Inoculation of Culture Media
- Incubation of Cultures
- Colony Characteristics
- Culture of Anaerobes
- Review Questions
- Key Points
- 42 Antimicrobial Sensitivity Testing
- Antibiotic Resistance
- Antimicrobial Sensitivity Testing
- Agar Diffusion Method
- Minimum Inhibitory Concentration
- Colony Count
- Review Questions
- Key Points
- 43 Additional Testing
- Motility
- Indole Test
- Catalase Test
- Coagulase Test
- Oxidase Activity
- Acid Production From Glucose
- California Mastitis Test
- Immunologic Examination
- Review Questions
- Key Points
- 44 Mycology
- Dermatophyte Testing
- Fungal Cultures
- Review Questions
- Key Points
- Unit 8 Parasitology
- Introduction
- 45 Nematodes
- Phylum Nematoda
- Review Questions
- Key Points
- 46 Cestodes, Trematodes, and Acanthocephalans
- Eucestodes
- Pseudotapeworms
- Trematodes
- Acanthocephalans (Thorny-Head Worms)
- Review Questions
- Key Points
- 47 Protozoa and Rickettsia
- Phylum Sarcomastigophora
- Phylum Apicomplexa
- Phylum Ciliophora
- Rickettsial Parasites
- Review Questions
- Key Points
- 48 Arthropods
- Order: Siphonaptera (Fleas)
- Orders: Mallophaga and Anoplura (Lice)
- Order: Diptera (Flies)
- Class: Acarina (Mites and Ticks)
- Pentastomids (Tongue Worms)
- Phylum: Annelida (Segmented Worms)
- Review Questions
- Key Points
- 49 Sample Collection and Handling
- Collection of Fecal Samples
- Skin Scraping
- Cellophane Tape Preparation
- Vacuum Collection
- Sample Collection at Necropsy
- Collection of Blood Samples
- Review Questions
- Key Points
- 50 Diagnostic Techniques
- Evaluation of Fecal Specimens
- Cellophane Tape Preparation
- Baermann Technique
- Miscellaneous Fecal Examinations
- Staining Procedures
- Evaluation of Blood Samples
- Immunologic and Molecular Diagnostic Tests
- Miscellaneous Parasitologic Evaluations
- Review Questions
- Key Points
- Unit 9 Cytology
- Introduction
- 51 Sample Collection and Handling
- Swabs
- Scrapings
- Imprints
- Fine-Needle Biopsy
- Tissue Biopsy
- Centesis
- Transtracheal/Bronchial Wash
- Concentration Techniques
- Review Questions
- Key Points
- 52 Preparation of Cytology Smears
- Smear Preparation
- Fixing and Staining the Cytology Sample
- Submission of Cytologic Preparations and Samples for Interpretation
- Review Questions
- Key Points
- 53 Microscopic Evaluation
- Inflammation
- Neoplasia
- Review Questions
- Key Points
- 54 Cytology of Specific Sites
- Peritoneal and Pleural Fluid
- Lymph Nodes
- Cerebrospinal Fluid
- Aqueous and Vitreous Humor
- Synovial Fluid Analysis
- Tracheal Wash
- Nasal Flush
- Ear Swabs
- Vaginal Cytology
- Fecal Cytology
- Semen Evaluation
- Evaluation of Prostatic Secretions
- Examination of Milk
- Review Questions
- Key Points
- Appendix A Review Questions
- Appendix B Reference Ranges
- Appendix C Bacterial Pathogens of Veterinary Importance
- Appendix D Professional Associations Related to Veterinary Clinical Laboratory Diagnostics
- Appendix E Common Parasites of Some Exotic Animal Species
- Parasites of Birds
- Parasites of Rabbits
- Parasites of Guinea Pigs
- Parasites of Rats
- Parasites of Mice
- Parasites of Hamsters
- Parasites of Gerbils
- Parasites of Fish
- Parasites of Reptiles
- Appendix F Example of a Standard Protocol for Reporting Results of a Urinalysis Laboratory Report
- Appendix G Taxonomic Classification of Parasites
- Kingdom: Animalia (Animals)
- Appendix H Zoonotic Internal Parasites
- Glossary
- Resources
- Index
- No. of pages: 433
- Language: English
- Edition: 7
- Published: December 13, 2018
- Imprint: Mosby
- Paperback ISBN: 9780323595384
- eBook ISBN: 9780323595032
MS
Margi Sirois
Margi Sirois is certified as both a veterinary technician & laboratory animal technician and has over 25 years of experience as a veterinary technician educator in both traditional and distance education programs. She is a past-president of the Association of Veterinary Technician Educators and the Kansas Veterinary Technician Association and co-founder and current president of the Academy of Veterinary Technician Specialists in Education
Affiliations and expertise
Consultant