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Laboratory Methods in Enzymology: Protein Part A

1st Edition, Volume 536 - January 8, 2014

Editor: Jon Lorsch

Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 4 2 0 0 7 0 - 8
eBook ISBN:
9 7 8 - 0 - 1 2 - 4 2 0 0 9 7 - 5

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volu… Read more

Laboratory Methods in Enzymology: Protein Part A

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The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.

This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including:

Pulse-chase analysis to measure protein degradation

Labeling a protein with fluorophores using NHS ester derivitization

Immunoaffinity purification of proteins

Proteolytic affinity tag cleavage

Purification of GST-tagged proteins