Laboratory Methods in Cell Biology
Biochemistry and Cell Culture
- 1st Edition, Volume 112 - November 28, 2012
- Latest edition
- Editor: P. Michael Conn
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 4 0 5 9 1 4 - 6
- eBook ISBN:9 7 8 - 0 - 1 2 - 4 0 5 5 4 9 - 0
Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questio… Read more
Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.
- Covers the most important laboratory methods in cell biology
- Chapters written by experts in their fields
Researchers and students in cell, molecular and developmental biology
Series Editors
Contributors
Series Page
Preface
Chapter 1. Extraction/Characterization of DNA
1 Purpose
2 Theory
3 Equipment (in order of appearance)
4 Materials (in order of appearance)
5 Protocol
References
Chapter 2. Co-Immunoprecipitation
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 3. Real-Time PCR
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Prepare Workspace
6 Protocol
References
Chapter 4. Single-Cell Gel Electrophoresis Combined with Lesion-Specific Enzymes to Measure Oxidative Damage to DNA
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 5. High-Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 6. Two-Dimensional Difference Gel Electrophoresis (2D DIGE)
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 7. Optimization of Cell Cycle Measurement by Time-Lapse Microscopy
1 Purpose
2 Theory
3 Determination of Cell Cycle Length
4 Equipments
5 Materials
6 Protocol
References
Chapter 8. Transfection Methods
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 9. Cell Enrichment from Human Blood through Red Cell Lysis
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 10. Magnetic, Batch Separation
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 11 Lateral Flow Sandwich Assay Utilizing Upconverting Phosphor (UCP) Reporters
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 12. In Vivo Modulation of MicroRNA Levels during Spinal Cord Regeneration
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 13. CometChip
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 14. Purification of Cardiac Cells
1 Theory
2 Equipments
3 Materials
4 Protocol
Reference
Chapter 15. Tube Formation In Vitro Angiogenesis Assay
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 16. Processing of Transmission Electron Microscopy Images for Single-Particle Analysis of Macromolecular Complexes
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 17. An Asymmetric 3D In Vitro Assay for the Study of Tumor Cell Invasion
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 18. Isolation of Myoblasts from Muscle Tissue by Preplating
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 19. High-Content Small Molecule Screening Strategies for Stem-Cell-Derived Motor Neurons
1 Theory
2 Equipment
3 Materials
4 Protocol
References
Chapter 20. A Simple Technique for Quantifying Apoptosis in 96-Well Plates
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 21. Cell Screening Using Resistive-Pulse Sensing
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 22. Quantitative Analysis of Basic Motile Behavior in Amoeboid Cells
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 23. Single Cell Deposition
1 Purpose
2 Theory
3 Equipments
4 Materials
5 Protocol
References
Chapter 24. Enzyme Sensors for Living Cells
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Chapter 25. Myoblast Purifying by Magnetic-Activated Cell Sorting (MACS)
1 Purpose
2 Theory
3 Equipment
4 Materials
5 Protocol
References
Subject Index
Volumes in Series
- Edition: 1
- Latest edition
- Volume: 112
- Published: November 28, 2012
- Language: English
PC
P. Michael Conn
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
Affiliations and expertise
Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center, TX, USARead Laboratory Methods in Cell Biology on ScienceDirect