Introduction to Protein Mass Spectrometry
- 2nd Edition - April 22, 2024
- Imprint: Academic Press
- Author: Pradip K. Ghosh
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 1 4 0 1 5 - 0
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 3 9 2 0 - 8
Introduction to Protein Mass Spectrometry, Second Edition provides a comprehensive overview of this increasingly important, yet complex, analytical technique. This book enables r… Read more
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Request a sales quoteIntroduction to Protein Mass Spectrometry, Second Edition provides a comprehensive overview of this increasingly important, yet complex, analytical technique. This book enables readers to understand how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. This new edition is fully updated to include the latest developments in the field and will feature new content covering recent progress in the areas where there have been the most exciting advances. These include PNNL’s multilevel-PCB-based SLIM realization, SLIM-Agilent QQQ field trials; employment of SLIM-IMS-cryo-IR combination in molecular structure determination; proximity-labelling mass spectrometry, and applications in neuroscience.
- Offers up-to-date, introductory information for scientists and researchers new to the field, as well as advanced insights into the critical assessment of computer-analyzed mass spectrometric results and their current limitations
- Provides examples of commonly used MS instruments from a range of key manufacturers/developers, including Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, Waters and PNNL
- Includes biological applications and exploration of analytical tools and databases for bioinformatics
- Features definitions, case studies, and recent developments in protein mass spectrometry
- Includes sections new to this edition on SLIM (Structures for Lossless Ion Manipulation) and mass spectrometry applications in neuroscience, including synaptic biology and Alzheimer’s disease
All scientists using Protein Mass Spectrometry, particularly analytical chemists, biochemists, drug discovery/medicinal chemists, biologists.
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Preface
- Preface to the Second Edition
- Chapter 1: Introduction
- Abstract
- Chapter 2: Sample Preparation and Ionization for Mass Spectrometry
- Abstract
- 2.1. Separation of protein mixtures
- 2.2. Multidimensional separations
- 2.3. Proteolysis
- 2.4. Ionization of proteins and peptides
- Chapter 3: Instruments
- Abstract
- 3.1. Mass analysis
- 3.2. Some examples of instruments
- Chapter 4: Mass Spectrometry of Peptides and Proteins
- Abstract
- 4.1. Proteins
- 4.2. Alternative analysis methods
- 4.3. Quantitation
- 4.4. Peptide fragmentation: Experiments
- 4.5. Data mining scheme for identifying peptide structural motifs
- 4.6. Peptide fragmentation: mechanism
- 4.7. Possible pathways in the competitionmodel
- 4.8. Studying post translational modifications
- 4.9. Chemical cross-linking/massspectrometry
- 4.10. Manual de novo sequencing of peptides
- Chapter 5: Examples from Biological Applications
- Abstract
- 5.1. Mapping intact protein isoforms using top-down proteomics
- 5.2. Quantitative analysis of intact apolipoproteins in human HDL
- 5.3. Rapid sequence analysis of some conotoxins—combination of de novo, bottom-up methods
- 5.4. Mass spectrometry of ribosomes
- 5.5. Proteins in Purkinje cell post-synaptic densities
- 5.6. Neurexin-LRRTM2 interaction effect in synapse formation
- 5.7. Rapid analysis of human plasma proteome—an IMS-IMS-MS application
- 5.8. Topology of two transient virus capsid assembly intermediates
- 5.9. Mass spectrometry of intact V-type ATPases reveals bound lipids and the effects of nucleotide binding
- 5.10. Mass spectrometric imaging of biological material
- 5.11. Immunopeptidomics
- 5.12. Metaproteomics
- 5.13. Proteogenomics
- 5.14. Single-cell proteomics and single-cell metabolomics
- 5.15. Applications in neurobiology
- Chapter 6: Mass Spectrometry-Based Bioinformatics
- Abstract
- 6.1. Peptides to proteins
- 6.2. MS/MS fragments to peptides to proteins
- 6.3. Data-dependent acquisition
- 6.4. Targeted acquisition covering SRM and PRM
- 6.5. Data-independent acquisition
- 6.6. Integrative mass spectrometry-based modeling
- Index
- Edition: 2
- Published: April 22, 2024
- Imprint: Academic Press
- No. of pages: 386
- Language: English
- Paperback ISBN: 9780443140150
- eBook ISBN: 9780443139208
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Pradip K. Ghosh
Pradip K. Ghosh received his PhD from Indiana University, USA, and did his postdoctoral research at Rice University. For most of his career he was on the faculty at the Indian Institute of Technology Kanpur, India. He also taught as a Visiting Professor at Pomona College and the University of California Berkeley. His research specialization has been in plasma chemical physics and in mass spectrometry. He is the author of several books, including Introduction to Photoelectron Spectroscopy (Wiley-Interscience), Ion Traps (Clarendon Press, Oxford), and the first edition of Introduction to Protein Mass Spectrometry (Academic Press).
Affiliations and expertise
Former Professor of Chemistry, Indian Institute of Technology, Kanpur, IndiaRead Introduction to Protein Mass Spectrometry on ScienceDirect