Integrated Methods in Protein Biochemistry: Part C
- 1st Edition, Volume 682 - March 20, 2023
- Latest edition
- Editor: Arun K. Shukla
- Language: English
Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting inter… Read more
Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology serials
- Updated release includes the latest information on Integrated Methods in Protein Biochemistry
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
1. NanoBiT-based methods to monitor the activation and modulation of RTKs
Andy Chevigne
2. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling
Ajith Karunarathne
3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems
Florian Schubot
4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase
Renwick Dobson
5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase
Thomas C. Brunold and Joshua Miller
6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms
John Robert Yates III
7. Covalent protein painting: MS-based protein footprinting
John Robert Yates III
8. Characterization of GPCR signaling complexes using negative-staining electron microscopy
Arun K. Shukla
9. Probing protein misfolding and dissociation with free electron laser
Kazuhiro Nakamura and Hisashi Okumura
10. Optimized protocol for the characterization of Cas12a activities
Rakhi Rajan, Lindsie Martin and Saadi Rostami
11. Proximity proteomics for the identification and characterization of extracellular vesicles
Norihiro Kotani
12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs)
Vincent Eijsink
13. Characterization of RRE domain in RiPP biosynthesis
Douglas A. Mitchell
14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations
Aaron T. Smith
15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging
Daniel Zicha
16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry
Nobuaki Takemori
17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins
Shawn Chen and Heng Wang
18. Quantitative cysteinome profiling with DIA-ABPP
Chu Wang
19. High-throughput screening and evolution of carbohydrate-active enzymes
Shishir PS Chundawat
20. Regulation of enzymes with light using unnatural amino acids
Andrea Christa Kneuttinger
21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies
Sven Eyckerman
22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics
Emily E. Weinert
23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors
Nicholas Alexander Graham
24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules
Joanna F. McGouran
25. Reconstitution of the MLL1 complex and probing methyltransferase activity
Yali Dou
26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy
Geert van den Bogaart
27. Structural and functional analysis of mono-copper enzymes from fungal species
28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins.
Daniel Dempsey
29. Electrophysiology of ion-transporting rhodopsins
Hideki Kandori
30. Measuring protein post-translational modifications using the protein semi-synthesis
Nam Chu
31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2)
Brian Patrick Weiser
32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP)
Tzu-Ching Meng
33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry
Nobuaki Takemori
34. Global substrate profiling of post-translational modifying enzymes
Charles S. Craik, Anthony O’Donoghue and Conner Bardine
35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide
Michael Knapp
Andy Chevigne
2. The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling
Ajith Karunarathne
3. Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems
Florian Schubot
4. Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase
Renwick Dobson
5. Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase
Thomas C. Brunold and Joshua Miller
6. DeGlyPHER: MS-based analysis of viral spike N-glycoforms
John Robert Yates III
7. Covalent protein painting: MS-based protein footprinting
John Robert Yates III
8. Characterization of GPCR signaling complexes using negative-staining electron microscopy
Arun K. Shukla
9. Probing protein misfolding and dissociation with free electron laser
Kazuhiro Nakamura and Hisashi Okumura
10. Optimized protocol for the characterization of Cas12a activities
Rakhi Rajan, Lindsie Martin and Saadi Rostami
11. Proximity proteomics for the identification and characterization of extracellular vesicles
Norihiro Kotani
12. Functional characterization of lytic polysaccharide monooxygenases (LPMOs)
Vincent Eijsink
13. Characterization of RRE domain in RiPP biosynthesis
Douglas A. Mitchell
14. The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations
Aaron T. Smith
15. Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging
Daniel Zicha
16. Gel-based sample preparation methods for top-down native/cross-linking mass spectrometry
Nobuaki Takemori
17. Identification and characterization of natural products and their interacts with anti-mycobacterial drug target proteins
Shawn Chen and Heng Wang
18. Quantitative cysteinome profiling with DIA-ABPP
Chu Wang
19. High-throughput screening and evolution of carbohydrate-active enzymes
Shishir PS Chundawat
20. Regulation of enzymes with light using unnatural amino acids
Andrea Christa Kneuttinger
21. An optimized protocol for modular cloning to rapidly assemble expression vectors for MS-based orthogonal interactomics studies
Sven Eyckerman
22. Nucleotide-linked resins to identify new nucleotide-binding proteins using proteomics
Emily E. Weinert
23. Phospho-proteomic analysis of immune cells expressing chimeric antigen receptors
Nicholas Alexander Graham
24. Novel strategies for targeting ubiquitin conjugation machinery using synthetic molecules
Joanna F. McGouran
25. Reconstitution of the MLL1 complex and probing methyltransferase activity
Yali Dou
26. Probing cellular aspects of endocytosis and phagocytosis with super-resolution microscopy
Geert van den Bogaart
27. Structural and functional analysis of mono-copper enzymes from fungal species
28. Structure, function and regulation of deubiquitinases and RNA-modifying proteins.
Daniel Dempsey
29. Electrophysiology of ion-transporting rhodopsins
Hideki Kandori
30. Measuring protein post-translational modifications using the protein semi-synthesis
Nam Chu
31. Assay design and analysis of human Uracil DNA Glycosylase (UNG2)
Brian Patrick Weiser
32. Structure and function of T-Cell Protein Tyrosine Phosphatase (TCPTP)
Tzu-Ching Meng
33. Gel-based sample preparation methods for top-down/native/cross-linking mass spectrometry
Nobuaki Takemori
34. Global substrate profiling of post-translational modifying enzymes
Charles S. Craik, Anthony O’Donoghue and Conner Bardine
35. Methods for measuring kinetic isotope effects on the hydroxylation of a peptide
Michael Knapp
- Edition: 1
- Latest edition
- Volume: 682
- Published: March 20, 2023
- Language: English
AS
Arun K. Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, IndiaRead Integrated Methods in Protein Biochemistry: Part C on ScienceDirect