Integrated Methods in Protein Biochemistry: Part C
- 1st Edition, Volume 682 - March 20, 2023
- Imprint: Academic Press
- Editor: Arun K. Shukla
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 1 8 5 9 2 - 2
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 1 8 5 9 3 - 9
Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting inter… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteIntegrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology serials
- Updated release includes the latest information on Integrated Methods in Protein Biochemistry
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title Page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter One: Nanoluciferase-based complementation assays to monitor activation, modulation and signaling of receptor tyrosine kinases (RTKs)
- Abstract
- 1: Introduction
- 2: Before you begin
- 3: Materials and equipment
- 4: Step-by-step method details
- 5: Expected outcome and quantification
- 6: Advantages
- 7: Limitations
- 8: Alternative methods/procedures
- Acknowledgments
- References
- Chapter Two: Molecular regulation of PLCβ signaling
- Abstract
- 1: Introduction
- 2: Structure of PLC
- 3: Isoforms and splice variants of PLC
- 4: Physiological and pathological role of PLC-mediated signaling
- 5: PLCβ activation and regulation
- 6: Synergistic activation of PLCβ by Gαq and Gβγ
- 7: Modulation of Gαq and Gβγ-governed PLCβ signaling
- 8: Cell-tissue-organ specific expression profiles of Gβγ and PLCβ
- 9: Conclusions
- Conflict of interests
- References
- Chapter Three: Laminar flow-based microfluidic systems for molecular interaction analysis—Part 1: Chip development, system operation and measurement setup
- Abstract
- 1: Introduction
- 2: Microfluidic device development
- 3: Fluidic actuation
- 4: Fluorescence detection
- 5: Summary and conclusions
- References
- Chapter Four: Spectroscopic analysis of the mammalian enzyme cysteine dioxygenase
- Abstract
- 1: Introduction
- 2: Spectroscopic analysis of CDO
- Acknowledgments
- References
- Chapter Five: DeGlyPHER: Highly sensitive site-specific analysis of N-linked glycans on proteins
- Abstract
- 1: Introduction
- 2: Before you begin
- 3: Key resources table
- 4: Materials and equipment
- 5: Step-by-step method details
- 6: Quantification and statistical analysis
- 7: Expected outcomes
- 8: Advantages
- 9: Limitations
- 10: Optimization and troubleshooting
- 11: Safety considerations and standards
- 12: Alternative methods/procedures
- Acknowledgments
- References
- Chapter Six: Sample preparation for structural mass spectrometry via polyacrylamide gel electrophoresis
- Abstract
- 1: Introduction
- 2: Sample preparation for top-down mass spectrometry by PEPPI-MS
- 3: Sample preparation for native mass spectrometry by PEPPI-MS
- 4: Sample preparation for cross-linking mass spectrometry by PEPPI-AnExSP
- 5: Summary and conclusions
- Acknowledgments
- References
- Chapter Seven: High-throughput screening of glycosynthases using azido sugars for oligosaccharides synthesis
- Abstract
- 1: Introduction
- 2: Screening using cyn regulon based azide detection method
- 3: Screening using click chemistry based azide detection method
- 4: Summary and conclusions
- Acknowledgments
- References
- Chapter Eight: Rational design, production and in vitro analysis of photoxenoproteins
- Abstract
- 1: Introduction
- 2: Rational design of photoxenoproteins
- 3: Synthesis of phenylalanine-4′-azobenzene AzoF
- 4: Heterologous gene expression and purification of photoxenoproteins
- 5: Characterization of photoxenoproteins
- 6: Evaluation of photocontrol
- 7: Notes
- Acknowledgments
- References
- Chapter Nine: Chemical and structural approaches to investigate PTEN function and regulation
- Abstract
- 1: Introduction
- 2: Biochemical assays to determine the conformational state of PTEN
- 3: Structural basis for N-terminal function and regulation
- 4: Structural basis for regulation of PTEN by C-terminal phosphorylation
- 5: Summary and future directions
- Acknowledgments
- References
- Chapter Ten: Analyzing protein posttranslational modifications using enzyme-catalyzed expressed protein ligation
- Abstract
- 1: Introduction
- 2: General methods and statistical analysis
- 3: Purification of subtiligase
- 4: Synthesis of peptide using solid phase peptide synthesis (SPPS)
- 5: Generation of POI C-terminal thioester
- 6: Enzyme-catalyzed EPL, and affinity purification of protein ligation product using biotin tag
- 7: Semisynthesis and biochemical analysis of phospholipid phosphatase PTEN with site-specific C-terminal phosphorylations
- 8: Summary and conclusions
- References
- Chapter Eleven: Application of hybrid biophysical-biochemical methods to unravel the molecular basis for auto-inhibition and activation of protein tyrosine phosphatase TCPTP/PTPN2
- Abstract
- 1: Introduction
- 2: Phosphatase assay
- 3: Chemical cross-linking coupled with mass spectrometry (CX-MS)
- 4: Hydrogen-deuterium exchange coupled with mass spectrometry (HDX-MS)
- 5: Validation of HDX-MS data by phosphatase assay
- 6: Conclusion
- Acknowledgments
- References
- Chapter Twelve: Multiplex substrate profiling by mass spectrometry for proteases
- Abstract
- 1: Introduction
- 2: General method and statistical analysis
- 3: Examples of MSP-MS in the literature
- 4: Conclusions and perspectives
- Acknowledgments
- References
- Bibliography of MSP-MS literature
- Chapter Thirteen: Protein labeling and crosslinking by covalent aptamers
- Abstract
- 1: Introduction
- 2: Equipment and materials
- 3: Protocol
- 4: Summary
- 5: General safety statement
- Acknowledgments
- References
- Chapter Fourteen: Laminar flow-based microfluidic systems for molecular interaction analysis—Part 2: Data extraction, processing and analysis
- Abstract
- 1: Introduction
- 2: Data extraction
- 3: Data processing
- 4: Data analysis
- 5: Summary and conclusions
- References
- Chapter Fifteen: A streamlined protocol for expression and purification of wild-type β-arrestins
- Abstract
- 1: Introduction
- 2: Materials, methods, and results
- 3: Potential problems and troubleshooting
- 4: Concluding remarks
- Acknowledgments
- References
- Edition: 1
- Volume: 682
- Published: March 20, 2023
- No. of pages (Hardback): 494
- No. of pages (eBook): 494
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780443185922
- eBook ISBN: 9780443185939
AS
Arun K. Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, IndiaRead Integrated Methods in Protein Biochemistry: Part C on ScienceDirect