Immobilized Enzyme Principles

List of Contributors

Introduction to the Series

Preface

Immobilized Enzymes—A Survey

Text

References

The Chemistry of Enzyme Immobilization

I. Introduction

II. Immobilization of Enzymes by Adsorption

III. Immobilization of Enzymes by Entrapment

IV. Immobilization of Enzymes by Covalent Cross-Linking with Bi- or Multifunctional Reagents

V. Immobilization of Enzymes by Covalent Coupling to Polymeric Supports

VI. Concluding Remarks

References

Diffusion and Kinetics with Immobilized Enzymes

I. Introduction

II. Factors Affecting the Kinetics of Bound Enzymes

III. Heterogeneous Enzyme Kinetics

IV. Effect of External Diffusional Limitations

V. Effect of Internal Diffusional Limitations

VI. Analysis of Diffusional Effects and Determination of Kinetic Parameters from Experimental Data

VII. Dynamic Effect of Weak Acids and Bases on Heterogeneous Enzyme Kinetics

VIII. Electrostatic Effects on Bound-Enzyme Kinetics

IX. Coimmobilized Multienzyme Systems

X. Perspectives

List of Symbols

References

Design and Analysis of Immobilized-Enzyme Flow Reactors

I. Introduction

II. Enzyme Reactor Types and Their Choice

III. Idealized Enzyme Reactor Systems

IV. Steady-State Analysis of Mass Transfer and Biochemical Reaction in Enzyme Reactors

V. Mass Transfer-Kinetic Models for Specialized Systems: Tubular Reactors with Catalytically Active Wall/Hollow-Fiber Reactors

VI. Nonideal Fluid-Flow Patterns in Enzyme Reactors

VII. Transient Analysis of Immobilized-Enzyme Reactors

VIII. Modeling of Multienzyme Reactor Systems

IX. Combined Reaction and Separation Processes

X. Process Optimization of Enzyme Reactors

XI. Comparison of Relative Efficiencies of Several Reactor Designs

XII. Epilog

List of Symbols

References

Industrial Applications of Immobilized Enzymes and Immobilized Microbial Cells

I. Introduction

II. Industrial Applications of Immobilized Enzymes

III. Applications of Immobilized Microbial Cells

IV. Conclusions

References

Subject Index