HLA from Benchtop to Bedside
- 1st Edition - January 5, 2021
- Imprint: Academic Press
- Author: A. Bradley Eisenbrey
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 3 9 7 6 - 6
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 4 2 3 7 - 7
HLA from Benchtop to Bedside provides the reader with a comprehensive, concise and thoroughly up-to-date book on all aspects of the HLA system, including new techniques and metho… Read more
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Request a sales quoteHLA from Benchtop to Bedside provides the reader with a comprehensive, concise and thoroughly up-to-date book on all aspects of the HLA system, including new techniques and methodologies. Each chapter begins with bullet point lists of principle learning points, including comprehensive references and validated links to international resources. Written by a diverse range of international academics for professionals, researchers, undergraduate and graduate students, this book is ideal for organ and stem cell transplant professionals, histocompatibility laboratory professionals and staff, medical residents and fellows on transplant services, medical students, and students in clinical laboratory science.
The book's author, Dr. Arthur Bradley Eisenbrey, is an experienced transplant pathologist who has held significant academic and leadership positions in the field.
- Reviews current knowledge surrounding the HLA system
- Covers current methodologies and utilization of histocompatibility testing
- Authored by a leader in the field of histocompatibility and transfusion medicine
Organ and stem cell transplant professionals, histocompatibility laboratory professionals and staff, medical residents and fellows on transplant services, medical students and students in clinical laboratory science
- Cover image
- Title page
- Table of Contents
- Copyright
- Preface
- Acknowledgments
- Chapter 1. Introduction and some history
- Fundamentals: innate and adaptive immunity
- Chapter 2. The structure of the major histocompatibility complex and major HLA components
- HLA class I
- Function of HLA Class Ia molecules
- HLA Ib molecules
- Other HLA class I histocompatibility genes: MICA and MICB
- HLA class II
- Chapter 3. Nomenclature and naming conventions for HLA
- HLA allele naming
- Chapter 4. The crossmatch
- Microcytotoxicity and the “NIH standard” technique
- Antihuman globulin
- Crossmatching by microcytotoxicity
- ABO and solid organ transplantation
- T and B cell isolation
- Immunofluorescence
- Flow cytometry
- HLA antibody detection in tissues
- Chapter 5. HLA antibody detection (screening) and identification
- Antibody identification by microlymphocytotoxicity
- Interpretation of antibody specificity in cell-based assays
- Panel reactive antibody
- Solid-phase antibody screening and identification
- Commercial solid-phase HLA antibody assays are qualitative, not quantitative
- Clinical indications for HLA antibody testing
- Single antigen bead result interpretation in clinical practice
- MFI as a quantitative result
- Virtual crossmatch
- Management of antibody-mediated transplant rejection
- HLA in Transfusion Medicine and Blood Banking
- Chapter 6. HLA typing by serologic methods
- Chapter 7. HLA typing by molecular methods
- Molecular HLA typing “before PCR”
- Oligonucleotide probing and endonuclease cutting
- Polymerase chain reaction and the molecular revolution in clinical medicine
- Polymerase chain reaction sequence specific primers
- PCR-RFLP
- PCR-rSSO
- High resolution typing ambiguities in molecular HLA typing
- The challenge of null alleles
- rtPCR (aka qPCR, real-time PCR, and quantitative PCR)
- Chapter 8. Massively parallel (next generation) sequencing
- NGS sequencing by Hybridization and Ligation
- Selection of molecular HLA typing methods
- Chapter 9. HLA alleles, disease associations, and HLA pharmacogenetics
- The mathematics of disease associations
- How do HLA alleles cause disease?
- Chapter 10. Engraftment monitoring
- A little history
- Microsatellite DNA, VNTRs, STRs
- Engraftment monitoring
- Engraftment monitoring by capillary electrophoresis
- Alternative platforms for engraftment monitoring
- Index
- Edition: 1
- Published: January 5, 2021
- Imprint: Academic Press
- No. of pages: 156
- Language: English
- Paperback ISBN: 9780128239766
- eBook ISBN: 9780128242377
AE
A. Bradley Eisenbrey
Arthur Bradley Eisenbrey, MD, PhD is a Clinical Associate Professor of Pathology, Wayne State University School of Medicine and the University of Toledo College of Medicine and Health Sciences. Semi-retired Transplant Immunologist and Specialist in Transfusion Medicine. He formerly directed Immunogenetics (HLA) laboratories at the Detroit Medical Center/Wayne State University, William Beaumont Hospital, Royal Oak, MI, Gift of Life Michigan and Henry Ford Hospital, Detroit, MI. Retired as a Colonel in the reserves of the United States Air Force. Board Certified in Anatomic and Clinical Pathology with subspecialty Board Certification in Blood Banking/Transfusion Medicine. Has served as chairperson of national committees of the AABB and College of American Pathologists (CAP) and continues to serve as a laboratory inspector for the CAP.
Affiliations and expertise
Clinical Associate Professor of Pathology, Wayne State University School of Medicine and the University of Toledo College of Medicine and Health SciencesRead HLA from Benchtop to Bedside on ScienceDirect