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Histochemical Techniques
- 2nd Edition - October 6, 1975
- Author: J. D. Bancroft
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 0 7 - 0 0 0 3 3 - 9
- Paperback ISBN:9 7 8 - 1 - 4 8 3 1 - 3 2 2 9 - 7
- eBook ISBN:9 7 8 - 1 - 4 8 3 1 - 6 4 7 1 - 7
Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze… Read more
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Request a sales quoteHistochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.
ForewordPreface1 Introduction2 Fixation3 Paraffin Sections4 Frozen Sections5 Cryostats6 Freeze Drying and Freeze Substitution7 Carbohydrates8 Proteins and Amino Acids9 Amyloid10 Lipids11 Nucleic Acids12 Pigments13 Minerals14 Principles of Enzyme Histochemistry15 Phosphatases16 Esterases17 Oxidoreductases I. Oxidases18 Oxidoreductases II. Dehydrogenases19 Miscellaneous Techniques20 Ultra-histochemistryAppendix I List of Methods II Preparation of Dyes and Mounting Media III Diazonium Salts IV Buffer Tables and Preparation of Molar and Normal Solutions V Some Sources of Supply for the Chemicals and Reagents Used in Methods Given in this BookIndex
- No. of pages: 358
- Language: English
- Edition: 2
- Published: October 6, 1975
- Imprint: Butterworth-Heinemann
- Hardback ISBN: 9780407000339
- Paperback ISBN: 9781483132297
- eBook ISBN: 9781483164717
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