High Pressure Liquid Chromatography

Preface

Abbreviations

Chapter 1. Introduction

I. Description

II. History

III. Comparison of Chromatographie Methods

IV. Advantages of High Pressure Liquid Chromatography

Chapter 2. Instrumentation

I. Hardware

II. Pumps

III. Detectors

IV. Columns

V. Recorders, Fraction Collectors, and Integrators

Chapter 3. Experimental Methods

I. Standardization of Conditions

II. Operating Parameters

III. Preparation of Sample

IV. Use of Fraction Collector

V. Automation

VI. Column Maintenance

VII. Instrument Maintenance

Chapter 4. Identification of Peaks

I. Use of Retention Data

II. Use of Internal Standards

III. Isotopic Labeling

IV. Collection and Characterization of Eluent Peaks

V. Enzymic Peak-Shift

VI. Derivatization

Chapter 5. Quantitation

I. Chromatography

II. Integration

III. Calculation

IV. Statistical Treatment of Data

Chapter 6. Applications

I. General Applications

II. Specific Applications

Bibliography

Author Index

Subject Index