Guide to Yeast Genetics: Functional Genomics, Proteomics and Other Systems Analysis

2nd Edition, Volume 470 - March 16, 2010

Editors: Christine Guthrie, Jonathan Weissman, Gerald R. Fink

Paperback ISBN:

9 7 8 - 0 - 1 2 - 3 7 5 1 7 1 - 3

This fully updated edition of the best-selling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the… Read more

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This fully updated edition of the best-selling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field.

PART I - Functional Genomics

1.Analysis of Gene Function using DNA Microarrays
Andrew P. Capaldi

2.An introduction to microarray data analysis and visualization
Gregg B. Whitworth

3. Genome-wide approaches to monitor pre-mRNA splicing
Maki Inada and Jeff Pleiss

4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites
Philippe Lefrançois, Wei Zheng and Michael Snyder

5. Genome-wide mapping of nucleosomes in yeast
Oliver J. Rando

6. Genome-wide translational profiling by ribosome footprinting
Nicholas T. Ingolia

PART II - Systematic Genetic Analysis

7. Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe
Anastasia Baryshnikova, Michael Costanzo, Scott Dixon, Franco J. Vizeacoumar, Chad L. Myers, Brenda Andrews and Charles Boone

8. Making temperature-sensitive mutants
Shay Ben-Aroya, Xuewen Pan, Jef D. Boeke and Philip Hieter

9. Quantitative Genetic Interaction Mapping using the E-MAP Approach
Sean R. Collins, Assen Roguev and Nevan J. Krogan

10. Exploring gene function and drug action using chemogenomic dosage assays
Elke Ericson, Shawn Hoon, Robert P. St. Onge, Guri Giaever and Corey Nislow

PART III - Proteomics

11. Yeast Expression Proteomics by High-Resolution Mass Spectrometry
Tobias C. Walther, Jesper V. Olsen and Matthias Mann

12. High-quality binary interactome mapping
Matija Dreze, Dario Monachello, Claire Lurin, Michael E. Cusick, David E. Hill, March Vidal, and Pascal Braun

13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics
Bernd Bodenmiller and Rudi Aebersold

14. A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells
Stephen W. Michnick, Po-Hien Ear, Christian Landry, Mohan K. Malleshaiah and Vincent Messier

15. Yeast Lipid Analysis and Quantification by Mass Spectrometry
Xue Li Guan, Isabelle Riezman, Markus R. Wenk and Howard Riezman

16. Mass Spectrometry-Based Metabolomics of Yeast
Christopher A. Crutchfield, Wenyun Lu, Eugene Melamud, and Joshua D. Rabinowitz

PART IV - Systems Analysis

17. Imaging single mRNA molecules in yeast
Hyun Youk, Arjun Raj and Alexander van Oudenaarden

18. Reconstructing gene histories in Ascomycota fungi
IlanWapinski and Aviv Regev

19. Experimental evolution in yeast: a practical guide
Maitreya J. Dunham

PART V - Synthetic Biology

20. Enhancing stress resistance and production phenotypes through transcriptome engineering
Felix H. Lam, Franz S. Hartner, Gerald R. Fink, and Gregory Stephanopoulos

PART VI - Advances in Cytology/Biochemistry

21. Visualizing yeast chromosomes and nuclear architecture
Peter Meister, Lutz R. Gehlen, Elisa Varela, Veronique Kalck and Susan M. Gasser

22. Quantitative localization of chromosomal loci by immunofluorescence
Donna Garvey Brickner, William Light and Jason H. Brickner

23. Spinning Disk Confocal Microscopy of Yeast
Kurt Thorn

24. Correlative GFP-immunoelectron microscopy in yeast
Christopher Buser and Kent McDonald

25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae
J Ross Buchan, Tracy Nissan and Roy Parker

26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization
Daniel Zenklusen and Robert H Singer

27. The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD)
Jeffrey L. Brodsky

28. A protein transformation protocol for introducing yeast prion particles into yeast
Motomasa Tanaka

29. Overexpression and Purification of Integral Membrane Proteins in Yeast
Franklin A. Hays, Zygy Roe-Zurz, and Robert M. Stroud

30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast
Simon Alberti, Randal Halfmann and Susan Lindquist

PART VII - Other Fungi

31. Genetics and Molecular Biology in Candida albicans
Hernday, A.D., Noble, S. M., Mitrovich, Q.M., and Johnson, A.D.

32. Molecular Genetics of Schizosaccharomyces pombe
Sarah A. Sabatinos and Susan L. Forsburg

33. Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans
Cheryl D. Chun and Hiten D. Madhani

34. The Fungal Genome Initiative and lessons learned from genome sequencing
Christina A. Cuomo and Bruce W. Birren

35. Ultradian metabolic cycles in yeast
Benjamin Tu