General methods, glycosaminoglycans, and glycoproteins

Contributors to Volume VIIPrefaceOutline of Volumes I-VIErrata and Additions—Volumes I-VISection I. Mono- and Disaccharides Preparation of Mono- and Disaccharides 1. D-Allose Glycosides and Nucleosides 2. Methyl α- and β-D-Glucoseptanosides 3. Methyl α-Maltoside 4. Preparation of 2′-Deoxyribonucleosides via Nucleoside Deoxyribosyl Transferase Amino Sugars 5. 2-Acetamido-2-Deoxy-D-Allose and 2-Acetamido-2-Deoxy-D-altrose 6. 2-Acetamido-2-Deoxy-D-Aulose and 2-Acetamido-2-Deoxy-D-Idose 7. 2-Acetamido-2-Deoxy-D-Galactose and 2-Acetamido-2-Deoxy-D-Talose Thio Sugars 8. Thio Sugars from Oxidatively Coupled Xanthates 9. Thio Sugars from Cyclic Thionocarbonates Oxidation Products 10. 3-Deoxy-D-manno-Octulosonic Acid Deoxyhalo Sugars 11. Direct Replacement of Primary Hydroxyl Groups by Halogen Esters 12. 1-O-Acetyl-2,3,5-Tri-O-Benzoyl-D-Arabino-Furanose and its Use in Glycosylation by Fusion Ethers and Anhydrosugars 13. Triphenylmethylation with Tritylpyridinium Flouroborate 14. 2,5-Anhydro-D-Mannitol Analysis 15. Radiochromatographic Analysis of Reducing Carbohydrates by Radiochromatography 16. Determination of Mono- and Disaccharides in Plant Materials 17. Determination of Anomeric Configuration by nmrSection II. Oligo- and Polysaccharides Isolation and Purification of Glycosaminoglycans 18. Heparitin (Heparan) Sulfate Isolation from Beef Lung Physical Methods of Analysis 19. Analysis and Characterization of Glycosaminoglycans by Nuclear Magnetic Resonance Spectroscopy 20. Microanalysis and Characterization of Glycosaminoglycans from Human Tissue via Zone Electrophoresis 21. Determination of the Molecular Weight of Connective Tissue Glycosaminoglycans (Acid Mucopolysaccharides) by Gel Electrophoresis 22. Circular Dichroism and Optical Rotatory Dispersion in Polysaccharide Structural Analysis: Intrinsic and Extrinsic Cotton Effects Chemical Methods of Analysis 23. Nitrous Acid Depolymerization of Glycosaminoglycans 24. Specific Degradation of Polysaccharides Containing Uronic Acid Residues 25. Stoichiometric Reduction of Uronic Acid Carboxyl Groups in Polysaccharides 26. Distribution of D-Galactopyranosyl Units in Galactomannans 27. Acyl Localization via Methyl ReplacementSection III. Glycoproteins Isolation and Purification 28. Isolation of Fetuin 29. Ribonuclease B 30. Ovalbumin of Chicken Egg White 31. Stem Bromelain Analysis of Glycoproteins 32. Isolation of Glycopeptides from Glycoproteins by Proteolytic Digestion 33. Isolation of Glycopeptides from Cell Membrane Glycoproteins 34. Acid-Catalyzed Hydrolysis and Methanolysis of Glycoproteins 35. Determination of O-Glycosidic Linkages to L-Serine and L-Threonine Residues of Glycoproteins 36. Determination of the 5-O-β-D-Galactopyranosylhydroxy-L-Lysine Linkage in Glycoproteins 37. Determination of the 4-N-2-Acetamido-2-Deoxy-β-D-Glucopyranosyl-L-Asparagine Linkage in Glycoproteins 38. Enzymic Methods for the Structure Analysis of the Carbohydrate Chain of Glycoproteins 39. Removal of Sialic Acids from Glycoproteins by Chemical Methods and Determination of Sialic Acids 40. Qualitative and Quantitative Determination of Sialic Acids 41. Determination of O-Acetyl Groups by the Hestrin Methods 42. Determination of Hexosamines and Hexosaminitols Using the Amino Acid Analyzer 43. Immunofluorescent Identification of Complex CarbohydratesSection IV. Selected Methods Found in Other CollectionsTextGlossaryAuthor IndexSubject Index