Gene Expression in Neural Tissues
- 1st Edition, Volume 9 - January 1, 1992
- Latest edition
- Editor: P. Michael Conn
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 3 7 6 5 - 7
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 5 9 6 5 - 9
Methods in Neurosciences, Volume 9: Gene Expression in Neural Tissues describes methods for the study of gene expression in neural tissues. Cloning, gene transfer, and Northern… Read more
Methods in Neurosciences, Volume 9: Gene Expression in Neural Tissues describes methods for the study of gene expression in neural tissues. Cloning, gene transfer, and Northern blotting for quantifying rare messenger RNAs are considered. The discussions are organized around five themes: expression of specific genes; in situ hybridization; gene transfer; regulation of expression; and general approaches. Comprised of 30 chapters, this volume first examines calbindin D28K gene expression in neurodegenerative diseases such as Parkinson's disease, Huntington's disease, and Alzheimer's disease, and the role of calbindin D28K in the etiology and pathogenesis of neurodegeneration in the brain. The reader is then introduced to gene expression of corticotropin-releasing factor, dystrophin, cytokines, and neurotrophic factors. Subsequent chapters focus on the gene expression of rat brain/Hep G2 glucose transporter, insulin-like growth factor II, and neuropeptide Y as well as proopiomelanocortin and neurotrophin receptors. The book also discusses gene regulation analysis by lipopolyamine-mediated DNA transfer in primary neurons; characterization of neuron-specific transcription factors in Drosophila melanogaster; and cloning of dopamine receptors using a homology approach. This monograph will be of interest to students and practitioners in the fields of molecular genetics, microbiology, neuroendocrinology, neurology, and other branches of the neurosciences.
Contributors to Volume 9
Preface
Volumes in Series
Section I Expression of Specific Genes
1. Calbindin D28K Gene Expression in Neurodegenerative Diseases
2. Corticotropin-Releasing Factor Gene Expression
3. Gene Expression of Cytokines and Neurotrophic Factors in Human Brain Tumors
4. Dystrophin Gene Expression in Neuronal Tissues
5. Neuropeptide FMRFamide Gene Expression
6. Rat Brain/Hep G2 Glucose Transporter Gene Expression in Brain
7. Insulin-Like Growth Factor II Gene Expression
8. Expression of Mouse Interphotoreceptor Retinoid-Binding Protein Gene during Development
9. Analysis of Muscarinic Acetylcholine Receptor Expression and Function
10. Expression of Human Neuropeptide Y Gene in Neuronal Cell Lines
11. Neuropeptide Y Gene Expression
12. Neurotrophin Receptor Gene Expression
13. Detection of Nerve Growth Factor and One of Its Receptors
14. Assessment of Proopiomelanocortin Gene Expression in Brain
15. Weaver Gene Expression in Central Nervous System
Section II In Situ Hybridization
16. In Situ Hybridization and Immunohistochemical Methods in Study of Regulatory Molecules
17. Using mRNA in Situ Hybridization to Localize Wnt-3 and Wnt-3A Expression in Developing Neural Tube
18. In Situ Hybridization for Neurofilament and GAP-43 mRNA in Primary Sensory Neurons in Conjunction with Nerve Growth Factor Receptor Radioautography
Section III Gene Transfer
19. Gene Regulation Analysis by Lipopolyamine-Mediated DNA Transfer in Primary Neurons
20. Retro virus-Mediated Gene Transfer into Mouse Cerebellar Cultures
21. Analysis of Cis-Acting Elements of Oxytocin Gene by DNA-Mediated Gene Transfer
Section IV Regulation of Expression
22. Identification of Cis-Regulatory Sequences Required for Induction of Neurotensin/Neuromedin N Gene Expression in PC12 Cells
23. Characterization of Neuron-Specific Transcription Factors in Drosophila melanogaster
24. DNA-Protein Interactions in Phenotypically Identified Neurons in Discrete Regions of Intact Brain
Section V General Approaches
25. Enhancer Trap Method in Drosophila: Its Application to Neurobiology
26. Use of Northern Blotting for Quantifying Rare mRNAs in Neurons of Developing Peripheral Nervous System
27. Dopamine D2 Receptors: Isolation of Cell Lines Using Ligand Binding
28. Cloning of Dopamine Receptors: Homology Approach
29. Cloning of Human Thyrotropin-Releasing Hormone cDNA Using Degenerate Oligonucleotide Probes and Tetramethylammonium Chloride
30. In Vivo Analysis of Gene Expression in Central Catecholamine Cells
Index
- Edition: 1
- Latest edition
- Volume: 9
- Published: January 1, 1992
- Language: English
PC
P. Michael Conn
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
Affiliations and expertise
Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center, TX, USARead Gene Expression in Neural Tissues on ScienceDirect