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G Protein-Coupled Receptors, Part B

  • 2nd Edition, Volume 149 - January 5, 2019
  • Latest edition
  • Editor: Arun K. Shukla
  • Language: English

G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality cha… Read more

Description

G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more.

Key features

  • Covers the increasingly appreciated cell biology field of G-protein-coupled receptors
  • Includes both established and new technologies
  • Contributed by experts in the field

Readership

Researchers and students in cell, molecular and developmental biology

Table of contents

1. Optical approaches for visualization of arrestin binding to muscarinic receptor
Seung Ryoung Jung

2. Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs
Hamiyet Unal

3. Assays to measure GPCR dependent cellular migration
Carole A. Parent

4. Characterization of the Frizzled GPCRs
Vladimir L. Katanaev

5. Binding assays for bradykinin and angiotensin receptors
Renan Martin

6. Detection of misfolded rhodopsin aggregates in cells
Paul Park

7. Measuring GPCR ubiquitination and trafficking
Samarjit Bhattacharyya

8. Culture of primary neurons and it use in studying GPCR trafficking
Prem N. Yadav

9. ELISA based assay for measuring agonist-induced GPCR endocytosis
Arun K. Shukla

10. Western blot assay for measuring agonist-induced ERK1/2 phosphorylation
Arun K. Shukla

11. Understanding GPCR dimerization
Agata Faron-Górecka

12. Investigating the Frizzled family of receptors
Vladimir L. Katanaev

13. Measuring GPCR signaling through xCELLigence system based assay
Laura Heitman

14. Novel mechanisms of GPCR signaling and bias
Gabriel Carmona Rosas

15. co-IP assays to measure GPCR-arrestin interactions
Anastasios Lymperopoulos

16. Investigating arrestin dependent GPCR signaling
Sadashiva Karnik

17. Measuring CRH1 receptor signaling
Susana Silberstein

18. Site-specific side chain labeling for NMR studies of G protein-coupled receptors
Brian F. Volkman

19. Modeling chemokine receptors and their orthosteric site interactions
Brian F. Volkman

20. Receptor-channel cross-talk
Uri Kahanovitch

21. Wnt receptor signaling
Nibaldo C. Inestrosa

Product details

  • Edition: 2
  • Latest edition
  • Volume: 149
  • Published: January 8, 2019
  • Language: English

About the editor

AS

Arun K. Shukla

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, India

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