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Elsevier

G Protein-Coupled Receptors Part A

  • 2nd Edition, Volume 142 - September 29, 2017
  • Latest edition
  • Editor: Arun K. Shukla
  • Language: English

G Protein Coupled Receptors, Second Edition, Volume 143, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters… Read more

Description

G Protein Coupled Receptors, Second Edition, Volume 143, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. It contains a wide array of topics about the G protein coupled receptors, as well as updates of chapters from the first edition.

Key features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on G protein coupled receptors
  • Includes analysis based topics

Readership

Researchers and students in cell, molecular and developmental biology

Table of contents

1. Generating recombinant peptide ligands for GPCRs and their single step purification

2. Ca2+ release assay to study downstream pathways of GPCR signal transduction

3. Expression and purification of GPCR interacting protein, arrestin

4. Isolation and characterization of chemosensory GPCRs from exosomes

5. Characterization of chemosensory bitter taste receptor signalling

6. Reporter gene assays for investigating GPCR signaling

7. Measuring binding affinity of regulatory motifs (peptides) to purified G proteins

8. Measuring nucleotide exchange on purified G proteins

9. Qualitative and quantitative measurement of GPCR-G protein activity in living cells

10. Computational approaches to study GPCR activation

11. Visualization and quantification of GPCR trafficking in mammalian cells by confocal microscopy

12. Computational studies of βarrestin activation

13. Measuring G protein coupling using Glosensor assay

14. Computational investigations into GPCR dimerization

15. cAMP Assays in GPCR Drug Discovery

16. Assays of adrenal GPCR signaling and regulation: Measuring adrenal beta-arrestin activity in vivo through plasma membrane recruitment

Product details

  • Edition: 2
  • Latest edition
  • Volume: 142
  • Published: October 2, 2017
  • Language: English

About the editor

AS

Arun K. Shukla

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, India

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