Frontiers in High Pressure Biochemistry and Biophysics

Preface. I. Introduction. High pressure effects on biological macromolecules: From structural changes to alteration of cellular processes (C. Balny et al.). II. Revisiting some basic concepts. Pressure-temperature phase diagrams of biomolecules (L. Smeller). The effects of osmotic and hydrostatic pressures on macromolecular systems (J.A. Kornblatt, M.J. Kornblatt). Compressibility of protein transitions (N. Taulier, T.V. Chalikian). Effects of high pressure on enzymatic activity (D.B. Northrop). III. Major developments in experimental methodology. UV-Visible derivative spectroscopy under high pressure (R. Lange, C. Balny). High pressure static fluorescence to study macromolecular structure-function (K. Ruan, C. Balny). Investigations of the effect of high hydrostatic pressure on proteins and lipidic membranes by dynamic fluorescence spectroscopy (P. Tauc et al.). Tryptophan phosphorescence and pressure effects on protein structure (P. Cioni, G.B. Strambini). Fourier transform infrared spectroscopy in high pressure studies on proteins (W. Dzwolak et al.). High resolution NMR studies of proteins (J. Jonas). Synchrotron X-ray and neutron small-angle scattering of lyotropic lipid mesophases, model biomembranes and proteins in solution at high pressure (R. Winter). High pressure simulations of biomolecules (E. Paci).IV. Folding, unfolding and molecular interactions : the high pressure approach. Revisiting volume changes in pressure-induced protein unfolding (C.A. Royer). Expanding the pressure technique: Insights into protein folding from combined use of pressure and chemical denaturants (S. Perrett, J.-M. Zhou). High hydrostatic pressure as a tool to study protein aggregation and amyloidosis (T.W. Randolph et al.). Pressure effects on intra- and intermolecular interactions within proteins (B.B. Boonyaratanakornkit et al.). Pressure induces folding intermediates that are crucial for protein-DNA recognition and virus assembly (J.L. Silva et al.). The interactions of nucleic acids at elevated hydrostatic pressure (R.B. Macgregor). Experimental and theoretical high pressure strategies for investigating protein-nucleic acid assemblies (T.W. Lynch, S.G. Sligar). V. Functionality of biosystems under high pressure. Effect of pressure on electron transfer reactions in inorganic and bioinorganic chemistry (J. Macyk, R. van Eldik). High pressure, a tool for exploring heme protein active sites (G. Hui Bon Hoa et al.). Cytochrome P450-CO and substrates. Lessons from ligand binding under high pressure (C. Jung). VI. The use of high pressure for applied biochemistry. Cold denaturation of proteins under high pressure (S. Kunugi, N. Tanaka). Protein crystallization under high pressure (Y. Suzuki et al.). The use of high pressure for separation and production of bioactive molecules (P. Lemay). Pressure effects on in vivo microbial processes (D.H. Bartlett). VII. Some snapshots for future prospects in basic and applied sciences. Compressibility gives new insight into protein dynamics and enzyme function (K. Gekko). Experiments on ion channels at high pressure (A.G. Macdonald). Cell biology and high pressure : applications and risks (H. Ludwig). Exploring hyperthermophilic proteins under pressure: theoretical aspects and experimental findings (E. Mombelli et al.). High pressure in bioscience and biotechnology : pure science encompassed in pursuit of value (R. Hayashi). Author index. Subject index.