Fluorescence Fluctuation Spectroscopy (FFS) Part B

Contributors

Preface

Methods in Enzymology

Chapter One. FCS in STED Microscopy

1 Introduction

2 STED-FCS of Lipid Membrane Dynamics

3 Lipid Membrane Dynamics

4 Conclusions

References

Further Reading

Chapter Two. Analyzing Förster Resonance Energy Transfer with Fluctuation Algorithms

1 Introduction

2 FRET and FCS

3 Filtered FCS

4 Applications

5 Discussion

References

Chapter Three. Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells

1 Introduction

2 Selected FFS Studies

3 Choice of Fluorophores: General Considerations

4 Cells: General Considerations

5 Summary

References

Chapter Four. Studying the Protein Corona on Nanoparticles by FCS

1 Introduction

2 Sample Preparation

3 Experimental Procedures

4 Data Analysis

5 Protein Corona Formation Measured by FCS

6 Conclusions

References

Chapter Five. Studying Antibody–Antigen Interactions with Fluorescence Fluctuation Spectroscopy

1 Introduction

2 Binding Model and Experimental Considerations

3 Studying Antibodies with FFS

4 Instrumentation

References

Chapter Six. Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling

1 Introduction

2 A Fluorescence Fluctuation Toolbox

3 Experimental Implementation

4 Applications

5 Conclusion

References

Chapter Seven. Interactions in Gene Expression Networks Studied by Two-Photon Fluorescence Fluctuation Spectroscopy

1 Introduction

2 In Vitro Interactions Between Proteins and Nucleic Acids Using Fluctuation Approaches

3 FFM in Live Bacterial Cells

4 Conclusions and Perspectives

References

Chapter Eight. Studying Ion Exchange in Solution and at Biological Membranes by FCS

1 Introduction

2 Ion Exchange Monitoring by FCS—Basic Approach

3 Monitoring of Local Ion Concentrations and Exchange in Solution

4 Monitoring of Proton Exchange at Biological Membranes by FCS

5 Approach for Ion Exchange Monitoring Incorporating Dual Color Fluorescence Cross Correlation Spectroscopy (FCCS)

6 Conclusions

References

Chapter Nine. Fluctuation Analysis of Activity Biosensor Images for the Study of Information Flow in Signaling Pathways

1 Introduction

2 Activity Biosensors

3 Extracting Activity Fluctuations in a Cell Shape Invariant Space

4 Correlation Analysis of Activity Fluctuations for Pathway Reconstruction

5 Outlook

References

Chapter Ten. Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy

Abbreviations

1 Introduction

2 Optical Setups for Sizing the Excitation Volume

3 General Considerations for svFCS Acquisition

4 Measurements on Living Cells

5 svFCS Data Analysis and Curve Fitting

6 The Nature of the Molecular Constraints on Lateral Diffusion

7 Summary and Future Outlook

References

Author Index

Subject Index