Fluorescence Fluctuation Spectroscopy (FFS), Part A
- 1st Edition, Volume 518 - December 28, 2012
- Imprint: Academic Press
- Editor: Sergey Tetin
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 3 8 8 4 2 2 - 0
- eBook ISBN:9 7 8 - 0 - 1 2 - 3 8 8 4 2 3 - 7
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers… Read more
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Request a sales quoteThis new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy
Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.
Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers fluorescence fluctuation spectroscopy
- Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Series page
Contributors
Preface
Methods in Enzymology
Chapter One. 40 Years of FCS: How It All Began
References
Chapter Two. Brief Introduction to Fluorescence Correlation Spectroscopy
1 Introduction
2 Conceptual Basis and Theory of FCS
3 Measurements Based on Fluctuation Amplitudes
4 Experimental Measurements
5 Close Relationship Between FCS and Fluorescence Photobleaching
6 Scanning and Imaging Approaches to FCS
7 A Brief and Partial Survey of Applications
8 Summary
References
Chapter Three. Dual-Color Fluorescence Cross-Correlation Spectroscopy with Continuous Laser Excitation in a Confocal Setup
1 Introduction
2 FCS in a Nutshell
3 The Dual-Color FCCS Extension
4 Data Analysis
5 Ligand Binding at the Cell Surface
6 Conclusion
References
Chapter Four. Brightness Analysis
1 Introduction
2 Background
3 Overview of Analysis Techniques
4 Brightness Analysis
5 Experimental Considerations
6 Brightness and Geometry
7 Summary
References
Chapter Five. Time-Integrated Fluorescence Cumulant Analysis and Its Application in Living Cells
1 Introduction
2 Theory and Implementation of TIFCA
3 Application of TIFCA
4 Conclusion
References
Chapter Six. Raster Image Correlation Spectroscopy and Number and Brightness Analysis
1 Introduction
2 A Conceptual Overview of Fluctuation Methods
3 What Is Different in the RICS Method?
4 RICS and Cross-RICS
5 PCH and Amplitude Fluctuation Analysis
6 N&B and Cross-NB
7 Simulations for Cross-RICS and Cross-N&B
8 Applications of RICS and N&B to Detect Molecular Complexes in Cells
9 Calibration Measurements using EGFP and mCherry and Effective Bleedthrough
10 Difference of Distribution in the Nucleus Versus Cytoplasm of Dynamin-2a
11 Conclusions and Future Prospects
12 Materials and Methods
References
Chapter Seven. Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy
1 Introduction
2 Background
3 Theory
4 Methods
5 Results
6 Conclusions
References
Chapter Eight. Dual-Focus Fluorescence Correlation Spectroscopy
1 Introduction
2 Dual-Focus Fluorescence Correlation Spectroscopy and Translational Diffusion
3 Flow Measurements with 2fFCS
4 2fFCS Measurements in Lipid Membranes
5 Summary
References
Chapter Nine. Pulsed Interleaved Excitation
1 Introduction: The Need for PIE
2 How Does PIE Work?
3 Juggling Photons with PIE
4 Simple and Quantitative Correlation Analysis with PIE
5 Quantifying FRET with PIE–FRET–FCCS
6 Accurate Single-Pair FRET with MFD–PIE
7 Scanning FCCS with PIE
8 Glancing at the Future for Multicolor Spectroscopy
References
Chapter Ten. Image Correlation Spectroscopy: Mapping Correlations in Space, Time, and Reciprocal Space
1 Introduction
2 Theory of ICS
3 Procedures for STICS and kICS
4 Conclusions
References
Author Index
Subject Index
- Edition: 1
- Volume: 518
- Published: December 28, 2012
- Imprint: Academic Press
- No. of pages: 336
- Language: English
- Hardback ISBN: 9780123884220
- eBook ISBN: 9780123884237
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