Evolving Landscape of Molecular Diagnostics

Applications and Techniques

1st Edition - June 27, 2024
Editors: Mrutyunjay Suar, Prashanth Singh, Namrata Misra
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 9 3 1 6 - 6
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 9 3 1 7 - 3

Evolving Landscape of Molecular Diagnostics: Applications and Techniques presents cutting-edge reviews on current and emerging technologies in the diagnosis of microbial infect… Read more

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Evolving Landscape of Molecular Diagnostics: Applications and Techniques presents cutting-edge reviews on current and emerging technologies in the diagnosis of microbial infections. The book discusses the fundamentals of Molecular Diagnostics for bacteria, fungi, viruses and a variety of state-of-the-art diagnostics tools and techniques for obtaining qualitative and quantitative results. It includes topics such as Next generation sequencing and application of "OMICS" for early disease diagnosis. Sections cover the entire spectrum of emerging diagnostic tools and techniques, as well as the principles, pros and cons of each method and applications for diagnosis of infectious disease in plants, humans and veterinary. This is a resource for both researchers and students working in clinical microbiology, infectious biology, applied life sciences and scientists working in the clinical diagnostic industry.

Cover image
Title page
Table of Contents
Copyright
Dedication
List of contributors
About the editors
Acknowledgment
Introduction
1 Scope and scientific emphasis of each chapter
2 Utility and distinctiveness of the book
A: Fundamentals of molecular diagnostics: from past to present to future
Chapter 1. Molecular diagnostics: past, present, and future
Abstract
1.1 Introduction
1.2 The past, the beginning, and the early milestones
1.3 Conclusion
References
Chapter 2. Molecular diagnostics for bacteria, virus, and fungi
Abstract
2.1 Introduction
2.2 Molecular methods for microbial diagnosis
2.3 Clinical virology and molecular diagnostics
2.4 Conclusion
References
B: Emerging diagnostics techniques: which one to choose?
Chapter 3. MALDI TOF-MS for microbial identification and diagnosis
Abstract
3.1 Introduction
3.2 Principle and methodology of MALDI TOF-MS
3.3 Advantages of MALDI-TOF-MS in comparison to alternative methods for microbial identification
3.4 Application of MALDI TOF-MS
3.5 Clinical application for the identification, taxonomy, and strain typing of bacteria and fungi
3.6 Other examples of MALDI-TOF-MS applications in the clinical diagnosis
3.7 Conclusion
References
Chapter 4. Microfluidics in diagnostic research: lab-on-a-chip technologies
Abstract
4.1 Introduction
4.2 Manipulating the fluid at microscale: microfluidic design
4.3 Fabrication of microfluidic devices
4.4 Applications of microfluidic devices
4.5 Lab-on-a-Chip devices in laboratory
4.6 Commercially available microfluidic systems
4.7 Way forward
References
Further reading
Chapter 5. TaqMan low-density arrays for simultaneous detection of multiple pathogens
Abstract
5.1 Introduction
5.2 TaqMan low-density array
5.3 Design of TaqMan low-density array card
5.4 TaqMan low-density array workflow
5.5 Advantages and disadvantages of TaqMan low-density array
5.6 Expanding RT-PCR assay to TaqMan low-density array
5.7 Importance of TaqMan low-density array in biomedical science
5.8 TaqMan array card in multipathogen assays
5.9 Comparison of TaqMan array card with RT-PCR assay and commercial multipathogen assays
5.10 Conclusion
References
Chapter 6. Molecular imaging: from the cellular players to bedside
Abstract
6.1 Introduction
6.2 Molecular imaging techniques, radiotracers, and their applications
6.3 Role of molecular imaging and diagnostics in pathological findings, therapy, and patient management
6.4 Future perspective: in vivo molecular imaging versus in vitro molecular diagnostics
References
Chapter 7. Semiconductor quantum dots for in vitro diagnostics and cellular imaging
Abstract
7.1 Introduction
7.2 Types and characteristics of quantum dots
7.3 Optical properties of quantum dots
7.4 Synthesis and characterization of quantum dots
7.5 Functionalization and solubilization
7.6 In vitro diagnostic applications
7.7 Other biological applications of quantum dots
7.8 Conclusion
Funding
References
Chapter 8. Loop-mediated isothermal amplification as a point of care diagnostic tool
Abstract
8.1 Revolutionizing diagnostics: the loop-mediated isothermal amplification technology
8.2 The mechanism of loop-mediated isothermal amplification in diagnostics
8.3 Empowering diagnostics: features of loop-mediated isothermal amplification technology
8.4 The versatility of loop-mediated isothermal amplification and its dynamic variants
8.5 Enhancing precision: approaches to monitor loop-mediated isothermal amplification results
8.6 Loop-mediated isothermal amplification: illuminating disease diagnosis with speed and precision
8.7 Loop-mediated isothermal amplification: revolutionizing diagnostics with cutting-edge advances
8.8 Shaping the future of diagnostics: advantages of loop-mediated isothermal amplification technology
8.9 Overcoming hurdles: challenges in harnessing the full potential of loop-mediated isothermal amplification
8.10 Loop-mediated isothermal amplification technology has the potential to lead the way in diagnostics
References
Chapter 9. Novel diagnostics techniques for detection of Coronavirus disease 2019
Abstract
9.1 Background
9.2 Targets for coronavirus disease 2019 detection (viral antigen, antibodies, and RNA)
9.3 Molecular-based testing methods
9.4 Conclusions
References
C: An overview of applications: human, plant, veterinary
Chapter 10. Next-generation sequencing and application of “omics” for early disease diagnosis
Abstract
10.1 Introduction
10.2 Genomics in disease diagnostics
10.3 Transcriptomics in disease diagnostics
10.4 Proteomics in disease diagnostics
10.5 Metabolomics in disease diagnostics
10.6 Microbiomics in disease diagnostics
10.7 Computational tools and data integration
10.8 Future selection methods
10.9 Clustering methods
10.10 Fusion methods
10.11 Biomarker identification and diagnosis
10.12 Conclusion
References
Chapter 11. Diagnostic techniques: clinical infectious diseases
Abstract
11.1 Introduction
11.2 Infectious diseases
11.3 Diagnostic techniques of infections
11.4 Future outlook
11.5 Conclusion
References
Chapter 12. Veterinary diagnostics: growth, trends, and impact
Abstract
12.1 Introduction
12.2 Types of veterinary diagnostics
12.3 Conventional methods and current trends for the detection of veterinary diseases
12.4 Challenges and ethical considerations
12.5 Conclusion
References
Index

Mrutyunjay Suar

Dr. Suar is a PhD in Molecular Microbiology from University of Delhi and Postdoc at ETH Zurich from 2004-2007. In June 2007 he joined KIIT University and established the School of Biotechnology. During this short span of time, the School of Biotechnology became a centre of excellence, supported by the Department of Biotechnology, Govt of India. Dr. Suar is involved in research related to interdisciplinary science with specialized focus on infection biology. His research group includes multidimensional researchers working on bacterial infection and development of vaccines. He is also involved in research related to nanobiotechnology focusing on development of nanomaterials for health application and understanding their toxicological aspects with different animal models. He established KIIT Technology Business Incubator (KIIT-TBI) in 2009 as the CEO, nurturing 200+companies in the area of engineering, energy, biotechnology and allied areas. He has received several recognitions including the American Society of Microbiology (ASM) Professorship award in 2012 during a visit to UCLA, USA; GP Chatterjee memorial award during 102nd Indian Science Congress and the Future Young Technical Leaders by STARS Switzerland in 2010. He also attended the Global Entrepreneurship leadership Symposium at HAAS Business School, USA and visited UC Berkley.

Affiliations and expertise

Director General R&D and KIIT University CEO, KIIT-Technology Business Incubator, Bhubaneswar, India

Prashanth Singh

Dr. Prashant PhD, Research Scientist. He has published articles in international journals and co-authored a book chapter with Springer. He has more than 10 years of techno-managerial experience in diverse areas of translational sciences including the fields of molecular diagnostics and next generation sequencing. Prior to joining BCKIC, he worked at FIND, where he was instrumental in capacity building projects on COVID-19 diagnostics including driving creation of appropriate SOPs, online and onsite training programs, workshops along with networking with academia and industries for disseminating knowledge on the rapid developments on COVID-19 diagnosis. He was a Product Manager for the South Asia region where he accelerated adaptation of multiple molecular assays including for HIV, HBV, HCV, HPV and blood screening. In addition to molecular diagnostics, Prashant managed whole genome sequencing and clinical sequencing assays for academic research and clinical findings for oncology, drug resistance, inherited diseases and non-invasive prenatal testing.

Affiliations and expertise

CEO, Bhubaneswar City Knowledge Innovation Cluster KIIT University, Bhubaneswar, India

Namrata Misra

Namrata Misra PhD received a M.Sc. degree in Bioinformatics from IIT (BHU) Varanasi, India, and a Ph.D. degree in Biological Science from AcSIR - Academy of Scientific & Innovative Research, CSIR-IMMT. She also received National Post-Doctoral Fellowship from Science and Engineering Research Board, Department of Science & Technology, Government of India. She is currently an Assistant Professor in the Department of Biotechnology, KIIT University. She is also the Head Bio innovations at KIIT Technology Business Incubator, among other key positions. Her research focus is on study of vital metabolic pathways and in-depth sequence-structural analysis of underpinning enzymes and also vaccine development using computational approach. She has also developed several cutting-edge computations tools, databases and webservers. She has authored or coauthored more than 30 international journal publications, and book chapters. She is the principal Investigator of a grant examining the metabolic pathways of algae using system biology approach for biofuel production. She has received the prestigious Odisha Young Scientist Award-2019 by Science and Technology Department of Government of Odisha and also the Best Researcher Award for 2012 at CSIR-IMMT, Bhubaneswar. Dr. Misra has led a number of workshops and training programs on innovation and entrepreneurship across several Institutes. She regularly mentors to students, startups and many young academicians to facilitate the translation of research discovery from lab to market and product development in healthcare sector. She has also visited to University of Edinburgh -UK under the Training-SinnoLABs Managers program in 2018 under the EU Erasmus Mundus Program. Also, she was selected to attend the ENPRENDIA Training of trainers and management meeting in Santiago de Compostela, Spain in January 2020.

Affiliations and expertise

Assistant Professor, KIIT School of Biotechnology; Head, Bio-innovations, KIIT Technology Business Incubator, KIIT Deemed to Be University, Bhubaneswar, India

Life Sciences

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Evolving Landscape of Molecular Diagnostics

Applications and Techniques

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Mrutyunjay Suar

Prashanth Singh

Namrata Misra