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Elsevier

Essential Zebrafish Methods: Genetics and Genomics

  • 1st Edition - September 5, 2009
  • Latest edition
  • Editors: H. William Detrich III, Leonard I. Zon, Monte Westerfield
  • Language: English

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research… Read more

Description

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.

Key features

  • Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors
  • Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error

Readership

Researchers in cell, developmental, and molecular biology, genetics

Table of contents

1. Haploid Screens and Gamma-Ray Mutagenesis
Charline Walker

2. Positional Cloning of Mutated Zebrafish Genes
Will Talbot

3. The Zebrafish Genome
John Postlethwait

4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish
Adam Amsterdam

5. Genetic Screens for Maternal-Effect Mutations
Francisco Pelegri

6. Behavior Screening Assays in Zebrafish
Herwig Baier

7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish
Cecelia B. Moens

8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element
Koichi Kawakami

9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish
Charles Lee

10. The Zon Laboratory Guide to Positional Cloning in Zebrafish
Leonard I. Zon

11. Sleeping Beauty Transposon for Efficient Gene Delivery
Stephen C. Ecker

12. Transgene Manipulation in Zebrafish by Using Recombinases
Gary W. Stuart

13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI
Joachim Wittbrodt

14. Cloning Zebrafish by Nuclear Transfer
Shuo Lin

15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening
Christine Thisse

16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish
Bill Trevarrow

17. Common Diseases of Laboratory Zebrafish
Jennifer L. Matthews

18. Zebrafish Sperm Cryopreservation
Thomas Look

Product details

  • Edition: 1
  • Latest edition
  • Published: September 5, 2009
  • Language: English

About the editors

HD

H. William Detrich III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
Affiliations and expertise
Professor of Biochemistry and Marine Biology at Northeastern University

LZ

Leonard I. Zon

Affiliations and expertise
Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA

MW

Monte Westerfield

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA
Affiliations and expertise
Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA